Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,513,468 T→C S218P (TCC→CCC)  ydcT → putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,513,4680TC100.0% 153.6 / NA 43S218P (TCC→CCC) ydcTputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/19);  total (24/19)

TGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACA  >  NC_000913/1513334‑1513601
                                                                                                                                      |                                                                                                                                     
tGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCcccc                                                                                                                                   >  2:1134084/1‑139 (MQ=255)
                               tctcGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAtct                                                                                                                          >  2:56216/1‑117 (MQ=255)
                               tctcGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAtct                                                                                                                          <  1:56216/117‑1 (MQ=255)
                                       tCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGc                                                                                            >  1:1233805/1‑139 (MQ=255)
                                             ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                      <  1:1134084/139‑1 (MQ=255)
                                             ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                      <  2:312402/139‑1 (MQ=255)
                                                    cGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACg                                                                                                       >  1:843359/1‑115 (MQ=255)
                                                    cGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACg                                                                                                       <  2:843359/115‑1 (MQ=255)
                                                            aCGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGaa                                                                       <  1:470729/139‑1 (MQ=255)
                                                              gATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATg                                                                     >  1:178902/1‑139 (MQ=255)
                                                                 cAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCa                                                                                                         >  2:220185/1‑100 (MQ=255)
                                                                 cAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCa                                                                                                         <  1:220185/100‑1 (MQ=255)
                                                                   gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                                >  2:1089729/1‑75 (MQ=255)
                                                                   gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                                <  1:1089729/75‑1 (MQ=255)
                                                                                     gTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCagag                                              >  1:796640/1‑139 (MQ=255)
                                                                                          aTCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                              <  1:625825/54‑1 (MQ=255)
                                                                                          aTCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                              >  2:625825/1‑54 (MQ=255)
                                                                                              tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgc                                                                                                                               >  1:230614/1‑49 (MQ=255)
                                                                                              tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgc                                                                                                                               <  2:230614/49‑1 (MQ=255)
                                                                                              tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATgcg                                                                                                                 >  2:969721/1‑63 (MQ=255)
                                                                                              tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATgcg                                                                                                                 <  1:969721/63‑1 (MQ=255)
                                                                                              tgtgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTtgt                                     >  2:698307/1‑139 (MQ=255)
                                                                                                tgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                                >  1:1049513/1‑46 (MQ=255)
                                                                                                tgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                                <  2:1049513/46‑1 (MQ=255)
                                                                                                tgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCcgcg                                                                                                           >  2:605441/1‑67 (MQ=255)
                                                                                                tgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCcgcg                                                                                                           <  1:605441/67‑1 (MQ=255)
                                                                                                tgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGc                                                                                            >  2:1041250/1‑82 (MQ=255)
                                                                                                tgGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGc                                                                                            <  1:1041250/82‑1 (MQ=255)
                                                                                                        ttCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACg                                                                                                       <  2:461146/63‑1 (MQ=255)
                                                                                                        ttCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACg                                                                                                       >  1:461146/1‑63 (MQ=255)
                                                                                                                  gACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                      >  1:638086/1‑70 (MQ=255)
                                                                                                                  gACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                      <  2:638086/70‑1 (MQ=255)
                                                                                                                    cGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCTGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGccc               >  1:1184637/1‑139 (MQ=255)
                                                                                                                       aTTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGa                                                                        <  1:696842/79‑1 (MQ=255)
                                                                                                                       aTTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGa                                                                        >  2:696842/1‑79 (MQ=255)
                                                                                                                        ttGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCggg                                                                                        >  1:649332/1‑62 (MQ=255)
                                                                                                                        ttGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCggg                                                                                        <  2:649332/62‑1 (MQ=255)
                                                                                                                           aGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGATGGTACATCGAATGTTTTTGATGGACTGATGACAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGAcc        >  2:930344/1‑139 (MQ=255)
                                                                                                                             cAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCgg      <  2:178902/139‑1 (MQ=255)
                                                                                                                               ggTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGtt                                                                                  <  1:88912/61‑1 (MQ=255)
                                                                                                                               ggTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGtt                                                                                  >  2:88912/1‑61 (MQ=255)
                                                                                                                                 tCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACa  >  1:115529/1‑139 (MQ=255)
                                                                                                                                 tCGATCCCCCGCGCGAGCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACa  >  2:1045781/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAACA  >  NC_000913/1513334‑1513601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑