Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 155,935 T→C K89K (AAA→AAG)  yadV ← putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913155,9350TC98.5% 245.0 / NA 68K89K (AAA→AAG) yadVputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (35/32);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTC  >  NC_000913/155799‑156060
                                                                                                                                        |                                                                                                                             
gcagctGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAATCAGAACACGCCCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGctt                                                                                                                             >  2:288356/1‑139 (MQ=255)
   gcagGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGc                                                                                                                          <  2:1429808/139‑1 (MQ=255)
    cagGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCa                                                                                                                         >  1:1384779/1‑139 (MQ=255)
     agGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                        <  2:1384779/139‑1 (MQ=255)
            gATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGACTGTTTGCCCACGCTTGGCATCAATAcg                                                                                                                 <  1:288356/139‑1 (MQ=255)
                     ccTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTgg                                                                                                        <  2:1319612/139‑1 (MQ=255)
                                tCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAc                                                                                                                  <  1:1082286/118‑1 (MQ=255)
                                tCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAc                                                                                                                  >  2:1082286/1‑118 (MQ=255)
                                 cTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGTCCACGCTTGGCATCAATa                                                                                                                   >  2:12558/1‑116 (MQ=255)
                                 cTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGTCCACGCTTGGCATCAATa                                                                                                                   <  1:12558/116‑1 (MQ=255)
                                   ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaa                                                                                          >  1:323520/1‑139 (MQ=255)
                                   ggTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaa                                                                                          >  2:620656/1‑139 (MQ=255)
                                    gTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTaaa                                                                                         <  2:323520/139‑1 (MQ=255)
                                        tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGACAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                        >  2:705336/1‑104 (MQ=255)
                                        tggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGACAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCAt                                                                                                                        <  1:705336/104‑1 (MQ=255)
                                              aaCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTa                                                                               >  2:1405944/1‑139 (MQ=255)
                                               aCTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTaa                                                                              <  1:708308/139‑1 (MQ=255)
                                                               aCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTa                                                                                                 >  2:1154128/1‑104 (MQ=255)
                                                               aCCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTa                                                                                                 <  1:1154128/104‑1 (MQ=255)
                                                                   gAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTg                                                          >  2:128543/1‑139 (MQ=255)
                                                                         gCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGctt                                                                                                                             >  2:292507/1‑66 (MQ=255)
                                                                         gCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGctt                                                                                                                             <  1:292507/66‑1 (MQ=255)
                                                                         gCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcgc                                                                                                                <  1:1256416/79‑1 (MQ=255)
                                                                         gCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATAcgc                                                                                                                >  2:1256416/1‑79 (MQ=255)
                                                                              ctctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGt                                               >  2:148292/1‑139 (MQ=255)
                                                                                ctGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTAt                                             <  1:128543/139‑1 (MQ=255)
                                                                                 tGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATc                                            >  1:1291005/1‑139 (MQ=255)
                                                                                   tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGtt                                                           >  1:515950/1‑122 (MQ=255)
                                                                                   tCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGtt                                                           <  2:515950/122‑1 (MQ=255)
                                                                                     tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATa                                                                            >  1:903685/1‑103 (MQ=255)
                                                                                     tttAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATa                                                                            <  2:903685/103‑1 (MQ=255)
                                                                                       tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTc                                                                 <  2:175519/112‑1 (MQ=255)
                                                                                       tAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTc                                                                 >  1:175519/1‑112 (MQ=255)
                                                                                         ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                <  1:939805/79‑1 (MQ=255)
                                                                                         ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                >  2:939805/1‑79 (MQ=255)
                                                                                         ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAAc                                    >  1:213599/1‑139 (MQ=255)
                                                                                         ggCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAAc                                    >  2:1322085/1‑139 (MQ=255)
                                                                                          gCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAAct                                   <  2:1291005/139‑1 (MQ=255)
                                                                                           cAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAActc                                  <  2:213599/139‑1 (MQ=255)
                                                                                             gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGt                                                                                                  <  2:1257058/73‑1 (MQ=255)
                                                                                             gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGt                                                                                                  >  1:1257058/1‑73 (MQ=255)
                                                                                             gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTg                                >  1:998609/1‑139 (MQ=255)
                                                                                             gTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCACAGCGTTGTCATCGCCAGTATCTAACCAACTCTg                                >  1:917496/1‑139 (MQ=255)
                                                                                              tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                <  1:108199/74‑1 (MQ=255)
                                                                                              tGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAg                                                                                                >  2:108199/1‑74 (MQ=255)
                                                                                               gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                      >  2:1088067/1‑83 (MQ=255)
                                                                                               gAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                      <  1:1088067/83‑1 (MQ=255)
                                                                                                   tGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCaa                                                                                                                     <  2:1059071/48‑1 (MQ=255)
                                                                                                   tGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCaa                                                                                                                     >  1:1059071/1‑48 (MQ=255)
                                                                                                   tGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAg                          >  2:1015587/1‑139 (MQ=255)
                                                                                                       ggctgTGTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAAc                      <  1:1322085/139‑1 (MQ=255)
                                                                                                          tgtgTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAAct                                   <  2:949564/123‑1 (MQ=255)
                                                                                                          tgtgTACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAAct                                   >  1:949564/1‑123 (MQ=255)
                                                                                                            tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                      >  2:1188732/1‑70 (MQ=255)
                                                                                                            tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAgg                                                                                      <  1:1188732/70‑1 (MQ=255)
                                                                                                            tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCAt                                                      >  2:1424743/1‑102 (MQ=255)
                                                                                                            tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCAt                                                      <  1:1424743/102‑1 (MQ=255)
                                                                                                            tgtACATTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGtt                 >  2:115144/1‑139 (MQ=255)
                                                                                                                 aTTAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTcc            <  1:1405944/139‑1 (MQ=255)
                                                                                                                  ttAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCg                                                             <  2:1403590/89‑1 (MQ=255)
                                                                                                                  ttAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCg                                                             >  1:1403590/1‑89 (MQ=255)
                                                                                                                  ttAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                  <  1:362550/100‑1 (MQ=255)
                                                                                                                  ttAATTTGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGcc                                                  >  2:362550/1‑100 (MQ=255)
                                                                                                                      tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGa                              <  2:705726/116‑1 (MQ=255)
                                                                                                                      tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGa                              >  1:705726/1‑116 (MQ=255)
                                                                                                                      tttGATTGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTa       <  2:448894/139‑1 (MQ=255)
                                                                                                                           ttGTTTGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTc  >  1:568281/1‑139 (MQ=255)
                                                                                                                               ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTaa                                                                              >  2:1251625/1‑59 (MQ=255)
                                                                                                                               ttGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTaa                                                                              <  1:1251625/59‑1 (MQ=255)
                                                                                                                                tGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGa                                                                                    <  1:1225785/52‑1 (MQ=255)
                                                                                                                                tGCCCACGCTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGa                                                                                    >  2:1225785/1‑52 (MQ=255)
                                                                                                                                        |                                                                                                                             
GCAGCAGGCTTTGATTCGCGACCTTTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTC  >  NC_000913/155799‑156060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑