Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,618,218	G→A	*397* (TAG→TAA)	ydeA →	arabinose efflux transporter, arabinose‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,618,218	0	G	A	100.0%	180.5 / NA	57	*397* (TAG→TAA)	ydeA	arabinose efflux transporter, arabinose‑inducible
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/29);  total (28/29)

GGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATC  >  NC_000913/1618084‑1618321                                                                                                                                       |                                                                                                        ggtaATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGa                                                                                                     <  1:187313/139‑1 (MQ=255)        aGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGccc                                                                                              >  2:183142/1‑139 (MQ=255)        aGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGccc                                                                                              >  2:247257/1‑139 (MQ=255)               tttGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCggg                                                                                       <  1:247257/139‑1 (MQ=255)                 tGCACTGGTTAATGTCGGTGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCTCGTTAATTTGGTCAATAATTTTATTTCCCCGCTGGCCAGTGACACTCGAAGAACATATGCAATTATTGAAGGGCCCATTCgggtc                                                                                     >  2:1164915/1‑137 (MQ=255)                 tGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                     <  1:183142/139‑1 (MQ=255)                           aaTGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATg                                                                           <  1:208041/139‑1 (MQ=255)                                  atgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                         <  2:769665/134‑1 (MQ=255)                                  atgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                         >  1:769665/1‑134 (MQ=255)                                  atgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGtt                                                                    >  2:482890/1‑139 (MQ=255)                                         gTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAAt                                                               >  2:332282/1‑137 (MQ=255)                                         gTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAAt                                                               <  1:332282/137‑1 (MQ=255)                                                   cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                     <  2:53043/105‑1 (MQ=255)                                                   cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                     >  2:187192/1‑105 (MQ=255)                                                   cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                     >  1:53043/1‑105 (MQ=255)                                                   cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGcc                                                                                     <  1:187192/105‑1 (MQ=255)                                                   cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGAt                                                   >  1:289243/1‑139 (MQ=255)                                                        tGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                              <  1:482890/139‑1 (MQ=255)                                                        tGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                              <  1:207793/139‑1 (MQ=255)                                                                  tGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataat                                       <  2:174589/136‑1 (MQ=255)                                                                  tGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataat                                       >  1:174589/1‑136 (MQ=255)                                                                       cgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                  <  2:744285/120‑1 (MQ=255)                                                                       cgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                  >  1:744285/1‑120 (MQ=255)                                                                                ggTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCcatac                      <  2:289243/139‑1 (MQ=255)                                                                                     aTCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCttt                                                                                  <  1:1035756/74‑1 (MQ=255)                                                                                     aTCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCttt                                                                                  >  2:1035756/1‑74 (MQ=255)                                                                                      tCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                         <  2:270270/82‑1 (MQ=255)                                                                                      tCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                         >  1:270270/1‑82 (MQ=255)                                                                                               ttCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTa                                                                        >  1:843756/1‑74 (MQ=255)                                                                                               ttCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTa                                                                        <  2:843756/74‑1 (MQ=255)                                                                                                tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                         >  1:190053/1‑72 (MQ=255)                                                                                                tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGt                                                                         <  2:190053/72‑1 (MQ=255)                                                                                                tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGAt                                                            <  2:269927/85‑1 (MQ=255)                                                                                                tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGAt                                                            >  1:269927/1‑85 (MQ=255)                                                                                                tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                               >  2:1068157/1‑114 (MQ=255)                                                                                                tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                               <  1:1068157/114‑1 (MQ=255)                                                                                                tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCacac                            <  1:890860/117‑1 (MQ=255)                                                                                                tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCacac                            >  2:890860/1‑117 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGc                                                                                      <  1:578828/58‑1 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGc                                                                                      >  2:578828/1‑58 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAgg                                                     <  2:1192313/91‑1 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAgg                                                     >  1:1192313/1‑91 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                              <  1:616336/98‑1 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                              >  2:616336/1‑98 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTta                                            >  1:611685/1‑100 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTta                                            <  2:611685/100‑1 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCacac                            >  2:257293/1‑116 (MQ=255)                                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCacac                            <  1:257293/116‑1 (MQ=255)                                                                                                    cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCAtt                                                                                           >  2:527152/1‑50 (MQ=255)                                                                                                    cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCAtt                                                                                           <  1:527152/50‑1 (MQ=255)                                                                                                    cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATc  >  2:505271/1‑139 (MQ=255)                                                                                                                   cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAgg                                                     <  2:650814/73‑1 (MQ=255)                                                                                                                   cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAgg                                                     >  1:650814/1‑73 (MQ=255)                                                                                                                   cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATAcc                 <  1:1065079/109‑1 (MQ=255)                                                                                                                   cTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACACATACATAcc                 >  2:1065079/1‑109 (MQ=255)                                                                                                                                cGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcaca                             >  2:1374682/1‑84 (MQ=255)                                                                                                                                cGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcaca                             <  1:1374682/84‑1 (MQ=255)                                                                                                                                       |                                                                                                        GGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATC  >  NC_000913/1618084‑1618321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑