Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,626,825	C→T	V186I (GTT→ATT)	dcp ←	dipeptidyl carboxypeptidase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,626,825	0	C	T	100.0%	119.9 / NA	39	V186I (GTT→ATT)	dcp	dipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/22);  total (17/22)

CGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAACGTT  >  NC_000913/1626694‑1626959                                                                                                                                    |                                                                                                                                       cgcCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccg                                                                                                                                 <  1:1182921/139‑1 (MQ=255)                       agcagcGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTa                                                                                                           >  2:1372778/1‑139 (MQ=255)                           gcGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCg                                                                                                       <  2:199121/139‑1 (MQ=255)                            cGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCAGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGc                                                                                                      >  2:545456/1‑139 (MQ=255)                              gAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTg                                                                                                    <  1:1372778/139‑1 (MQ=255)                              gAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTg                                                                                                    >  2:1162116/1‑139 (MQ=255)                                        tttGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAActgg                                                                                          <  1:612030/139‑1 (MQ=255)                                                   gACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCg                                                                                                       >  1:1175797/1‑115 (MQ=255)                                                   gACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCg                                                                                                       <  2:1175797/115‑1 (MQ=255)                                                    aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAActggctg                                                                                       <  1:550253/130‑1 (MQ=255)                                                    aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAActggctg                                                                                       >  2:550253/1‑130 (MQ=255)                                                    aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTc                                                                              >  2:713735/1‑139 (MQ=255)                                                    aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTc                                                                              >  2:29807/1‑139 (MQ=255)                                                     ccTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATc                                                                                                        >  2:1422135/1‑112 (MQ=255)                                                     ccTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATc                                                                                                        <  1:1422135/112‑1 (MQ=255)                                                     ccTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCg                                                                             <  1:29807/139‑1 (MQ=255)                                                        ttCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                          <  1:545456/139‑1 (MQ=255)                                                        ttCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                          <  1:1162116/139‑1 (MQ=255)                                                        ttCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                          <  1:1183292/139‑1 (MQ=255)                                                         tctcGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                          <  2:610875/138‑1 (MQ=255)                                                         tctcGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                          >  1:610875/1‑138 (MQ=255)                                                               gAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTcagt                                                                   <  1:713735/139‑4 (MQ=255)                                                                     ccTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtg                                                                                                                    >  1:1279617/1‑84 (MQ=255)                                                                     ccTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtg                                                                                                                    <  2:1279617/84‑1 (MQ=255)                                                                                  cgcAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCt                                                                     >  2:252585/1‑118 (MQ=255)                                                                                  cgcAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCt                                                                     <  1:252585/118‑1 (MQ=255)                                                                                  cgcAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAAtttt                                                >  2:988360/1‑139 (MQ=255)                                                                                  cgcAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAAtttt                                                >  1:643593/1‑139 (MQ=255)                                                                                   gcAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTg                                               <  2:643593/139‑1 (MQ=255)                                                                                     aaTCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccgc                                                                                                                                <  1:678170/56‑1 (MQ=255)                                                                                     aaTCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccgc                                                                                                                                >  2:678170/1‑56 (MQ=255)                                                                                       tctcTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCttt                                           >  1:492214/1‑139 (MQ=255)                                                                                                 actcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAAt                                                   <  2:173656/121‑1 (MQ=255)                                                                                                 actcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAAt                                                   >  1:173656/1‑121 (MQ=255)                                                                                                       tCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAAct                                                                                            <  2:565187/74‑1 (MQ=255)                                                                                                       tCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAAct                                                                                            >  1:565187/1‑74 (MQ=255)                                                                                                                   cgcgATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCa               <  2:1038455/139‑1 (MQ=255)                                                                                                                             ttCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAAc     <  1:988360/139‑1 (MQ=255)                                                                                                                                acaATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAACGtt  <  2:492214/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       CGCCGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAACGTT  >  NC_000913/1626694‑1626959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑