Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,665,120	G→A	*205* (TAG→TAA)	dmsD →	twin‑argninine leader‑binding protein for DmsA and TorA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,665,120	0	G	A	100.0%	180.2 / NA	56	*205* (TAG→TAA)	dmsD	twin‑argninine leader‑binding protein for DmsA and TorA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/29);  total (27/29)

ACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGT  >  NC_000913/1664985‑1665249                                                                                                                                        |                                                                                                                                  acGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGAt                                                                                                                                <  2:1176273/139‑1 (MQ=255)   gTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCa                                                                                                                              <  2:71970/139‑1 (MQ=255)                 tttATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCg                                                                                                                >  2:153062/1‑139 (MQ=255)                  ttATCGAAATAGCAGAACACCCTTTCTACCGTGCTCTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCgg                                                                                                               <  1:917123/139‑1 (MQ=255)                   tATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGa                                                                                                              <  1:153062/139‑1 (MQ=255)                         aaaaGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGa                                                                                                        <  2:1402708/139‑1 (MQ=255)                                 aaCACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcg                                                                                                <  2:301469/139‑1 (MQ=255)                                      ccTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCaa                                                                                           >  2:1353144/1‑139 (MQ=255)                                        tttCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATAGCAATCAAAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATc                                                                                         >  2:1395845/1‑139 (MQ=255)                                         ttCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATcc                                                                                        <  1:951217/139‑1 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTTCCGGATGGTGACGATTACGCGCAATCCgg                                                                                      <  2:542864/134‑1 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCgg                                                                                      >  1:542864/1‑134 (MQ=255)                                                gTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                 >  2:951938/1‑139 (MQ=255)                                                  gCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaataat                                                                               <  2:1342446/139‑1 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATcc                                                                                        >  1:335523/1‑124 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATcc                                                                                        >  1:1249643/1‑124 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATcc                                                                                        <  2:1249643/124‑1 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATcc                                                                                        <  2:335523/124‑1 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa                                                                         >  2:258548/1‑139 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa                                                                         >  1:293087/1‑139 (MQ=255)                                                        ggTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTa                                                                         >  1:1408584/1‑139 (MQ=255)                                                          tGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACa                                                                       <  2:1408584/139‑1 (MQ=255)                                                             aCTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAgtg                                                                    >  1:1163309/1‑139 (MQ=255)                                                                  cTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGc                                                                                     >  1:1155713/1‑117 (MQ=255)                                                                  cTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGc                                                                                     <  2:1155713/117‑1 (MQ=255)                                                                         aaCGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                        <  1:951938/139‑1 (MQ=255)                                                                                   cAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTaaa                                              <  2:184703/139‑1 (MQ=255)                                                                                     aTGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAgc                                            >  2:451484/1‑139 (MQ=255)                                                                                     aTGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAgc                                            >  1:210632/1‑139 (MQ=255)                                                                                      tGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCtt                                                 <  1:1388496/133‑1 (MQ=255)                                                                                      tGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCtt                                                 >  2:1388496/1‑133 (MQ=255)                                                                                          aaTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcg                                                                                                >  2:281298/1‑82 (MQ=255)                                                                                          aaTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcg                                                                                                <  1:281298/82‑1 (MQ=255)                                                                                           aTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAgcacgcac                                      <  2:87363/139‑1 (MQ=255)                                                                                             cacaACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTg                                    >  2:1170639/1‑139 (MQ=255)                                                                                                     ttAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTAt                                                      >  2:122289/1‑113 (MQ=255)                                                                                                     ttAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTAt                                                      <  1:122289/113‑1 (MQ=255)                                                                                                      tAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg                           <  1:451484/139‑1 (MQ=255)                                                                                                       aaTTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                        <  1:754261/109‑1 (MQ=255)                                                                                                       aaTTCCTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttt                                                        >  2:754261/1‑109 (MQ=255)                                                                                                            cTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                 >  2:1371155/1‑79 (MQ=255)                                                                                                            cTGTCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                 <  1:1371155/79‑1 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcgcg                                                                                              >  2:656850/1‑63 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTAcgcg                                                                                              <  1:656850/63‑1 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                 <  2:1038265/76‑1 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCaata                                                                                 >  1:1038265/1‑76 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAgca                                           <  2:174791/114‑1 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAgca                                           >  1:174791/1‑114 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTg                              <  2:619474/127‑1 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTg                              >  1:619474/1‑127 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg                           >  1:766269/1‑130 (MQ=255)                                                                                                               tCGCGGTTAAACCGCTGTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg                           <  2:766269/130‑1 (MQ=255)                                                                                                                               gTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCg                                                                                       >  2:501661/1‑54 (MQ=255)                                                                                                                               gTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCg                                                                                       <  1:501661/54‑1 (MQ=255)                                                                                                                               gTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGt  >  2:1257081/1‑139 (MQ=255)                                                                                                                               gTTTCGATAAGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGt  <  1:1170639/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  ACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGT  >  NC_000913/1664985‑1665249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑