Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,733,703	G→A	*1539* (TAG→TAA)	lhr →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,733,703	0	G	A	100.0%	152.3 / NA	48	*1539* (TAG→TAA)	lhr	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/25);  total (23/25)

TGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGA  >  NC_000913/1733573‑1733829                                                                                                                                   |                                                                                                                               tGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGa                                                                                                                        >  2:680321/1‑139 (MQ=255)   cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACt                                                                                                                      >  1:109333/1‑139 (MQ=255)                                aaCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATTCCCGTTCGCATCCGTCAGTAt                                                                                         >  1:842547/1‑139 (MQ=255)                                aaCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTAt                                                                                         >  2:645778/1‑139 (MQ=255)                                      aGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                   <  2:109333/139‑1 (MQ=255)                                       gTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGa                                                                                  <  1:645778/139‑1 (MQ=255)                                                 tACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTc                                                                        <  2:709598/139‑1 (MQ=255)                                                      gTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCGCAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGt                                                                   >  1:975126/1‑139 (MQ=255)                                                       tCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTc                                                                  >  2:146763/1‑139 (MQ=255)                                                        ccGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGat                                                                             <  1:1215013/127‑1 (MQ=255)                                                        ccGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGat                                                                             >  2:1215013/1‑127 (MQ=255)                                                        ccGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTcc                                                                 >  1:774272/1‑139 (MQ=255)                                                         cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTc                                                                                              >  1:1058024/1‑109 (MQ=255)                                                         cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTc                                                                                              <  2:1058024/109‑1 (MQ=255)                                                         cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                >  2:291788/1‑139 (MQ=255)                                                         cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                >  2:485867/1‑139 (MQ=255)                                                         cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                >  2:69502/1‑139 (MQ=255)                                                             aaaCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGctc                                                            >  1:1133831/1‑139 (MQ=255)                                                               aCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGTTTATTCCGCGTCCGGCTCtt                                                          <  2:842547/139‑1 (MQ=255)                                                               aCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCtt                                                          <  1:69502/139‑1 (MQ=255)                                                                 gCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                        <  1:146763/139‑1 (MQ=255)                                                                    gATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGAc                                                     <  1:291788/139‑1 (MQ=255)                                                                    gATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGAc                                                     <  1:1314465/139‑1 (MQ=255)                                                                             gctgctGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGAc                                                     >  1:1074827/1‑130 (MQ=255)                                                                             gctgctGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGAc                                                     <  2:1074827/130‑1 (MQ=255)                                                                                gctgcGTGAGGTGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGctc                                                            <  1:725975/120‑1 (MQ=255)                                                                                gctgcGTGAGGTGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGctc                                                            >  2:725975/1‑120 (MQ=255)                                                                                gctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCg                                         <  1:680321/139‑1 (MQ=255)                                                                                  tgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                                       >  2:45829/1‑139 (MQ=255)                                                                                    cGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAg                                     <  1:485867/139‑1 (MQ=255)                                                                                      tGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACg                                                                                                                  >  2:45831/1‑60 (MQ=255)                                                                                      tGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACg                                                                                                                  <  1:45831/60‑1 (MQ=255)                                                                                                 tttCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAg                        <  1:45829/139‑1 (MQ=255)                                                                                                         tCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCa                <  2:1133831/139‑1 (MQ=255)                                                                                                          cGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAg               >  2:1386239/1‑139 (MQ=255)                                                                                                           gCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                   <  2:197119/70‑1 (MQ=255)                                                                                                           gCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                   >  1:197119/1‑70 (MQ=255)                                                                                                            ccACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGtt             >  2:369553/1‑139 (MQ=255)                                                                                                               caAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc          <  2:1369246/139‑1 (MQ=255)                                                                                                               caAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc          <  1:1386239/139‑1 (MQ=255)                                                                                                               caAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc          <  1:1025504/139‑1 (MQ=255)                                                                                                               caAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc          <  1:369553/139‑1 (MQ=255)                                                                                                                       ggATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGa  >  2:653883/1‑139 (MQ=255)                                                                                                                       ggATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGa  <  1:653883/139‑1 (MQ=255)                                                                                                                              ggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                               >  1:1159920/1‑71 (MQ=255)                                                                                                                              ggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                               <  2:1159920/71‑1 (MQ=255)                                                                                                                              ggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGAc                                                     >  2:1284528/1‑81 (MQ=255)                                                                                                                              ggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGAc                                                     <  1:1284528/81‑1 (MQ=255)                                                                                                                                   |                                                                                                                               TGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGA  >  NC_000913/1733573‑1733829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑