Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,575	G→A	intergenic (+14/+26)	cfa → / ← ribC	cyclopropane fatty acyl phospholipid synthase, SAM‑dependent/riboflavin synthase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,575	0	G	A	100.0%	192.1 / NA	61	intergenic (+14/+26)	cfa/ribC	cyclopropane fatty acyl phospholipid synthase, SAM‑dependent/riboflavin synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/31);  total (30/31)

TGTTTACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCC‑GGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCA  >  NC_000913/1742449‑1742697                                                                                                                               |                                                                                                                            tGTTTACCTATTATCTGAATGCCTGTGCAGGTTCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCC‑gg                                                                                                                >  2:849082/1‑139 (MQ=255)           ttatCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGAttt                                                                                                       >  2:1317025/1‑139 (MQ=255)             atCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCa                                                                                                     >  2:27769/1‑139 (MQ=255)                tGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGAt                                                                                                  <  2:1262835/139‑1 (MQ=255)                     gCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGc                                                                                             >  1:1135772/1‑139 (MQ=255)                     gCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGc                                                                                             >  1:839805/1‑139 (MQ=255)                      ccTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATc                                                                                                 <  1:804972/134‑1 (MQ=255)                      ccTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATc                                                                                                 >  2:804972/1‑134 (MQ=255)                       cTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCtt                                                                                           <  2:677633/139‑1 (MQ=255)                        tgtgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTc                                                                                          >  1:735760/1‑139 (MQ=255)                         gtgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCt                                                                                         <  2:735760/139‑1 (MQ=255)                          tgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCa                                                                                                <  1:212348/131‑1 (MQ=255)                          tgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCa                                                                                                >  2:212348/1‑131 (MQ=255)                          tgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCtg                                                                                        >  2:967373/1‑139 (MQ=255)                          tgCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCtg                                                                                        >  2:603742/1‑139 (MQ=255)                            cAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTTTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCtgtg                                                                                      >  2:110759/1‑139 (MQ=255)                             aGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGc                                                                                     <  2:1135772/139‑1 (MQ=255)                               gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCt                                                                                   >  1:1315111/1‑139 (MQ=255)                                  cTTTCCGCTCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGTTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt                                                                                <  1:849082/139‑1 (MQ=255)                                  cTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt                                                                                >  2:534435/1‑139 (MQ=255)                                  cTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt                                                                                <  2:548564/139‑1 (MQ=255)                                  cTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGt                                                                                >  1:1344801/1‑139 (MQ=255)                                         cgcCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCa                                                                         <  2:1228573/139‑1 (MQ=255)                                           cccGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATg                                                                       <  2:1095951/139‑1 (MQ=255)                                                         ctctGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgc                                                         >  1:693604/1‑139 (MQ=255)                                                         ctctGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgc                                                         <  1:1067799/139‑1 (MQ=255)                                                         ctctGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgc                                                         >  1:263489/1‑139 (MQ=255)                                                            tGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgccgc                                                      >  1:831396/1‑139 (MQ=255)                                                            tGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgccgc                                                      >  2:442518/1‑139 (MQ=255)                                                            tGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgccgc                                                      >  2:85026/1‑139 (MQ=255)                                                            tGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgccgc                                                      <  1:442518/139‑1 (MQ=255)                                                             ggCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAccc                                                                                                                          >  1:281394/1‑70 (MQ=255)                                                             ggCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCAccc                                                                                                                          <  2:281394/70‑1 (MQ=255)                                                                     gtgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACg                                             <  2:1315111/139‑1 (MQ=255)                                                                     gtgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACg                                             <  2:1344801/139‑1 (MQ=255)                                                                      tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGAGc                                                                                     >  2:813320/1‑98 (MQ=255)                                                                      tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGAGc                                                                                     <  1:813320/98‑1 (MQ=255)                                                                        ttCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCC‑GGGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTg                     <  1:683114/139‑1 (MQ=255)                                                                          ctcACGCGGTGTTGAAAACGGCCTTCGAGCGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa                                        <  1:967373/139‑1 (MQ=255)                                                                            cACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                      <  1:1317025/139‑1 (MQ=255)                                                                            cACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                      <  2:839805/139‑1 (MQ=255)                                                                            cACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                      <  1:110759/139‑1 (MQ=255)                                                                                  gtgtTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCt                                >  2:100605/1‑139 (MQ=255)                                                                                      tGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCa                            <  1:1327223/139‑1 (MQ=255)                                                                                          aaCGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGc                        <  1:534435/139‑1 (MQ=255)                                                                                             ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCAt                                                                        >  2:1404593/1‑88 (MQ=255)                                                                                             ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCAt                                                                        <  1:1404593/88‑1 (MQ=255)                                                                                             ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTg                     <  2:648329/139‑1 (MQ=255)                                                                                             ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTg                     >  1:648329/1‑139 (MQ=255)                                                                                             ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTg                     >  2:145147/1‑139 (MQ=255)                                                                                             ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCC‑gggggg                                                                                                            >  2:365583/1‑50 (MQ=255)                                                                                             ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCC‑gggggg                                                                                                            <  1:365583/51‑2 (MQ=255)                                                                                               ccTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACg                                             >  2:1308185/1‑113 (MQ=255)                                                                                               ccTTCGAGTGGCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACg                                             <  1:1308185/113‑1 (MQ=255)                                                                                                         gCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCa                                                                                                >  2:1311231/1‑52 (MQ=255)                                                                                                         gCTCGCTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCa                                                                                                <  1:1311231/52‑1 (MQ=255)                                                                                                              cTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgccg                                                       <  2:765392/88‑1 (MQ=255)                                                                                                              cTAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTgccg                                                       >  1:765392/1‑88 (MQ=255)                                                                                                               tAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCt                      <  2:117726/120‑1 (MQ=255)                                                                                                               tAAAGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCt                      >  1:117726/1‑120 (MQ=255)                                                                                                                aaaGGCTATTCTATCACCCCCTCTCCGGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCa  <  1:27769/139‑1 (MQ=255)                                                                                                                               |                                                                                                                            TGTTTACCTATTATCTGAATGCCTGTGCAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCC‑GGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCA  >  NC_000913/1742449‑1742697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑