Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,789,804	G→A	*64* (TAG→TAA)	ydiE →	hemin uptake protein HemP homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,789,804	0	G	A	100.0%	172.7 / NA	55	*64* (TAG→TAA)	ydiE	hemin uptake protein HemP homolog
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/26);  total (29/26)

TTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCA  >  NC_000913/1789686‑1789931                                                                                                                       |                                                                                                                                ttCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGa                                                                                                             >  2:1347811/1‑139 (MQ=255)      cGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTcc                                                                                                        <  1:266643/139‑1 (MQ=255)        aaTTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAg                                                                                                      <  2:1343117/139‑1 (MQ=255)                           ttAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGt                                                                                   >  1:619576/1‑139 (MQ=255)                              ggTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGga                                                                                >  1:331988/1‑139 (MQ=255)                               gTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgac                                                                               >  2:891105/1‑139 (MQ=255)                                   ggATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAATCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTc                                                                                       <  1:811530/127‑1 (MQ=255)                                   ggATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTc                                                                                       >  2:811530/1‑127 (MQ=255)                                     aTGGCAAGCTGATTATCGATCATGAGGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCt                                                                                                               <  2:920883/101‑1 (MQ=255)                                     aTGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCt                                                                                                               >  1:920883/1‑101 (MQ=255)                                     aTGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTcc                                                                                                        >  2:853417/1‑108 (MQ=255)                                     aTGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTcc                                                                                                        <  1:853417/108‑1 (MQ=255)                                       ggCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                       <  1:823275/139‑1 (MQ=255)                                         cAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATa                                                                     <  2:619576/139‑1 (MQ=255)                                                          aTGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGc                                                    >  1:184574/1‑139 (MQ=255)                                                                  cAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTc                                                                                       <  2:507180/96‑1 (MQ=255)                                                                  cAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTc                                                                                       >  1:507180/1‑96 (MQ=255)                                                                  cAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGa                                            >  1:520754/1‑139 (MQ=255)                                                                  cAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGACCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                       <  2:648721/112‑1 (MQ=255)                                                                  cAAGAATATCTGCTACGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                       >  1:648721/1‑112 (MQ=255)                                                                         atCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCa                                     >  1:536955/1‑139 (MQ=255)                                                                          tCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCa                                                                                          >  2:935278/1‑85 (MQ=255)                                                                          tCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCa                                                                                          <  1:935278/85‑1 (MQ=255)                                                                          tCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCaa                                    <  1:891105/139‑1 (MQ=255)                                                                             gCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTA‑CCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGcc                                >  2:1254100/1‑139 (MQ=255)                                                                               tCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTCTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCt                               >  1:271052/1‑139 (MQ=255)                                                                                ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCTCAGTCAGGTg                                                                                  >  2:1026297/1‑87 (MQ=255)                                                                                ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTg                                                                                  <  1:1026297/87‑1 (MQ=255)                                                                                ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgac                                                                               <  1:537851/90‑1 (MQ=255)                                                                                ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgac                                                                               >  2:537851/1‑90 (MQ=255)                                                                                ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                       <  2:304970/98‑1 (MQ=255)                                                                                ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                       >  1:304970/1‑98 (MQ=255)                                                                                 cGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgac                                                                               >  1:137188/1‑89 (MQ=255)                                                                                 cGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgac                                                                               <  2:137188/89‑1 (MQ=255)                                                                                   tAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATg                           >  1:354757/1‑139 (MQ=255)                                                                                           aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGa                                                 >  2:739343/1‑109 (MQ=255)                                                                                           aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGa                                                 <  1:864549/109‑1 (MQ=255)                                                                                           aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGa                                                 >  2:864549/1‑109 (MQ=255)                                                                                           aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGa                                                 <  1:739343/109‑1 (MQ=255)                                                                                            ggCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTg                  <  2:184574/139‑1 (MQ=255)                                                                                            ggCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTg                  <  2:670239/139‑1 (MQ=255)                                                                                             gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTg                                                        >  2:1195168/1‑100 (MQ=255)                                                                                             gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTg                                                        <  1:1195168/100‑1 (MQ=255)                                                                                             gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAgg                       <  2:440387/133‑1 (MQ=255)                                                                                             gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGCACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAgg                       >  1:440387/1‑133 (MQ=255)                                                                                                ggCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAAt              >  2:1208322/1‑139 (MQ=255)                                                                                                 gCATGCTGCTGTTGACCACGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTAc                                                                                              <  2:943485/58‑1 (MQ=255)                                                                                                 gCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTAc                                                                                              >  1:943485/1‑58 (MQ=255)                                                                                                       tgctgTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCa       >  1:259333/1‑139 (MQ=255)                                                                                                          tgTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTCTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATACAGGCGGTGCAATTCCGTCatat    <  2:271052/139‑1 (MQ=255)                                                                                                            ttGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCa  <  2:520754/139‑1 (MQ=255)                                                                                                                ccAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGt                                                                                                  <  2:69712/39‑1 (MQ=255)                                                                                                                ccAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGt                                                                                                  >  1:69712/1‑39 (MQ=255)                                                                                                                   aGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTAcc                                                                                             <  2:70171/41‑1 (MQ=255)                                                                                                                   aGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTAcc                                                                                             >  1:70171/1‑41 (MQ=255)                                                                                                                       |                                                                                                                                TTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCA  >  NC_000913/1789686‑1789931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑