Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,799,883 T→C K39K (AAA→AAG)  rpmI ← 50S ribosomal subunit protein L35

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,799,8830TC100.0% 124.3 / NA 35K39K (AAA→AAG) rpmI50S ribosomal subunit protein L35
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/16);  total (19/16)

CTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACT  >  NC_000913/1799755‑1800015
                                                                                                                                |                                                                                                                                    
ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCtt                                                                                                                            >  2:1334230/1‑139 (MQ=255)
       gTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGt                                                                                                                     <  2:290126/139‑1 (MQ=255)
             atatTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAAt                                                                                                               >  2:1273756/1‑139 (MQ=255)
                     aTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGc                                                                                                        >  1:623016/1‑138 (MQ=255)
                     aTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGc                                                                                                        <  2:623016/138‑1 (MQ=255)
                      ttAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAg                                                                                                      >  2:883960/1‑139 (MQ=255)
                                  aaaCGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgctt                                                                                          <  1:838734/139‑1 (MQ=255)
                                      gTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgcttgtgc                                                                                      >  1:1139375/1‑139 (MQ=255)
                                        tAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATATGACGCAGGTTAGCgtgcttgtgctt                                                                                    >  2:709725/1‑139 (MQ=255)
                                             gCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAac                                                                               <  1:465989/139‑1 (MQ=255)
                                                      tACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCg                                                                                                                               >  1:470968/1‑82 (MQ=255)
                                                      tACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCg                                                                                                                               <  2:470968/82‑1 (MQ=255)
                                                       aCGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTAcc                                                                     >  1:42738/1‑139 (MQ=255)
                                                        cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGt                                                                                                    <  2:154511/107‑1 (MQ=255)
                                                        cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGt                                                                                                    >  1:154511/1‑107 (MQ=255)
                                                        cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCg                                                                    >  1:495033/1‑139 (MQ=255)
                                                        cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCg                                                                    >  1:866675/1‑139 (MQ=255)
                                                        cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCg                                                                    >  1:1028031/1‑139 (MQ=255)
                                                              gcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCtttt                                                                                                                          <  1:1424992/79‑1 (MQ=255)
                                                              gcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCtttt                                                                                                                          >  2:1424992/1‑79 (MQ=255)
                                                              gcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgcttg                                                                                         >  1:87983/1‑112 (MQ=255)
                                                              gcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgcttg                                                                                         <  2:87983/112‑1 (MQ=255)
                                                              gcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGttttt                                                              >  1:738753/1‑139 (MQ=255)
                                                                      ttACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                      <  1:883960/139‑1 (MQ=255)
                                                                      ttACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                      <  1:1273756/139‑1 (MQ=255)
                                                                           aGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTag                                                 <  2:866675/139‑1 (MQ=255)
                                                                                       ccTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCa                                                                                                       <  2:286257/73‑1 (MQ=255)
                                                                                       ccTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCa                                                                                                       >  1:286257/1‑73 (MQ=255)
                                                                                       ccTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTa                                     <  2:1028031/139‑1 (MQ=255)
                                                                                       ccTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTa                                     <  2:42738/139‑1 (MQ=255)
                                                                                       ccTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTa                                     <  2:495033/139‑1 (MQ=255)
                                                                                        cTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTAc                                    <  2:1139375/139‑1 (MQ=255)
                                                                                                           cGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCAtt                 >  2:702184/1‑139 (MQ=255)
                                                                                                                gCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTtaat            >  1:504292/1‑139 (MQ=255)
                                                                                                                  aGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTtaataa          >  2:1091571/1‑139 (MQ=255)
                                                                                                                       acgtttacgcttGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTcc     <  1:702184/139‑1 (MQ=255)
                                                                                                                          tttacgcttGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACt  <  1:1091571/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                    
CTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACT  >  NC_000913/1799755‑1800015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑