Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,814,212	C→T	H116Y (CAC→TAC)	katE →	catalase HPII, heme d‑containing

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,814,212	0	C	T	100.0%	127.3 / NA	40	H116Y (CAC→TAC)	katE	catalase HPII, heme d‑containing
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/20);  total (20/20)

AAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCCACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAAT  >  NC_000913/1814093‑1814342                                                                                                                        |                                                                                                                                   aaaaTTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCAt                                                                                                                 >  2:1386683/1‑139 (MQ=255)               aCCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTGTTCTGCGCGAGAAAATCACCTACTTTGACCATGa                                                                                                                       <  2:700762/119‑1 (MQ=255)               aCCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTGTTCTGCGCGAGAAAATCACCTACTTTGACCATGa                                                                                                                       >  1:700762/1‑119 (MQ=255)               aCCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTg                                                                                                   >  2:457588/1‑139 (MQ=255)                       tCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCa                                                                                           <  2:816390/139‑1 (MQ=255)                           ggCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTc                                                                                                >  2:663551/1‑130 (MQ=255)                           ggCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTc                                                                                                <  1:663551/130‑1 (MQ=255)                              gTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAgcg                                                                                                                    >  2:707440/1‑107 (MQ=255)                              gTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAgcg                                                                                                                    <  1:707440/107‑1 (MQ=255)                                gcgcATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCa                                                                                  >  1:1398629/1‑139 (MQ=255)                                gcgcATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCa                                                                                  >  1:1271488/1‑139 (MQ=255)                                      cGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCt                                                                            <  2:1271488/139‑1 (MQ=255)                                            cgaTCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGt                                                                      <  2:799692/139‑1 (MQ=255)                                                     cTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAg                                                             >  1:1240979/1‑139 (MQ=255)                                                          tGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGa                                                                                                           >  1:636835/1‑88 (MQ=255)                                                          tGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGa                                                                                                           <  2:636835/88‑1 (MQ=255)                                                             gTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATaa                                                     <  1:457588/139‑1 (MQ=255)                                                                   gTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCtt                                               >  1:700317/1‑139 (MQ=255)                                                                    tAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTa                                              <  1:1386683/139‑1 (MQ=255)                                                                    tAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTa                                              >  2:249173/1‑139 (MQ=255)                                                                      gCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAg                                            <  1:292407/139‑1 (MQ=255)                                                                      gCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAg                                            >  2:292407/1‑139 (MQ=255)                                                                            gTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGatat                                      >  2:72856/1‑139 (MQ=255)                                                                                aaCGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGc                                                                                            >  2:389638/1‑81 (MQ=255)                                                                                aaCGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGc                                                                                            <  1:389638/81‑1 (MQ=255)                                                                                       ctgGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAg                                                             >  1:71931/1‑105 (MQ=255)                                                                                       ctgGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATaa                                                     <  2:1269495/113‑1 (MQ=255)                                                                                       ctgGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATaa                                                     >  1:1269495/1‑113 (MQ=255)                                                                                       ctgGAAGATTTTATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGAATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAg                                                             <  2:71931/105‑1 (MQ=255)                                                                                           aaGATTTTATTCTGCGCGCGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGAttt                       <  2:687608/139‑1 (MQ=255)                                                                                           aaGATTTTATTCTGCGCAAGAAAATCACCTACTTTGACCCTGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGAttt                       <  2:1038282/139‑1 (MQ=255)                                                                                               ttttATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGc                                                                             >  2:178952/1‑81 (MQ=255)                                                                                               ttttATTCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGc                                                                             <  1:178952/81‑1 (MQ=255)                                                                                                     tCTGCGCGAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGAt             >  1:104962/1‑139 (MQ=255)                                                                                                         cgcgGGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTC‑TGCACGCGGATCAGCCGCTCACGGTTATTTc                                                                >  2:1214535/1‑83 (MQ=255)                                                                                                         cgcgGGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTC‑TGCACGCGGATCAGCCGCTCACGGTTATTTc                                                                <  1:1214535/83‑1 (MQ=255)                                                                                                         cgcgAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGa         >  2:413361/1‑139 (MQ=255)                                                                                                          gcgAGAAAATCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGaa        <  1:413361/139‑1 (MQ=255)                                                                                                               aaaaTCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACaaaa   <  2:104962/139‑1 (MQ=255)                                                                                                                aaaTCACCTACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAAt  <  2:1240979/139‑1 (MQ=255)                                                                                                                        |                                                                                                                                   AAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCCACTTTGACCATGAGCGCATTCCGGAACGTATTGTTCATGCACGCGGATCAGCCGCTCACGGTTATTTCCAGCCATATAAAAGCTTAAGCGATATTACCAAAGCGGATTTCCTCTCAGATCCGAACAAAAT  >  NC_000913/1814093‑1814342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑