Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,834,111	G→A	*237* (TAG→TAA)	ydjX →	TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,834,111	0	G	A	100.0%	177.8 / NA	56	*237* (TAG→TAA)	ydjX	TVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/28);  total (28/28)

TACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTTTCGAA  >  NC_000913/1833981‑1834246                                                                                                                                   |                                                                                                                                        tACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGt                                                                                                                                 <  1:844602/139‑1 (MQ=255)        ctgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAAc                                                                                                                           <  1:406527/138‑1 (MQ=255)        ctgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAAc                                                                                                                           >  2:406527/1‑138 (MQ=255)        ctgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                          <  1:478487/139‑1 (MQ=255)           tctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTCCTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACatta                                                                                                                       >  1:1395304/1‑139 (MQ=255)               gCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCa                                                                                                                   <  2:1066096/139‑1 (MQ=255)                cGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAg                                                                                                                  >  2:1423139/1‑139 (MQ=255)                     cTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                          >  2:1398295/1‑126 (MQ=255)                     cTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                          <  1:1398295/126‑1 (MQ=255)                                      cGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGc                                                                                            >  1:423468/1‑139 (MQ=255)                                       gTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATTTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAACACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCt                                                                                           <  2:1395304/139‑1 (MQ=255)                                        tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAACAATGACGGATAAAACGCTCTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTg                                                                                          >  2:1297012/1‑139 (MQ=255)                                        tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTg                                                                                          >  1:1252081/1‑139 (MQ=255)                                          cAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               <  2:751123/100‑1 (MQ=255)                                          cAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               >  1:751123/1‑100 (MQ=255)                                           aGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc                                                                                       <  1:607841/139‑1 (MQ=255)                                            gCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTTCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAct                                                                                      <  2:929483/139‑1 (MQ=255)                                            gCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAct                                                                                      <  2:878372/139‑1 (MQ=255)                                            gCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAct                                                                                      <  2:1252081/139‑1 (MQ=255)                                             cTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCActc                                                                                     >  1:630386/1‑139 (MQ=255)                                             cTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCActc                                                                                     <  2:630386/139‑1 (MQ=255)                                                   aaaCTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTa                                                                               <  1:1102106/139‑1 (MQ=255)                                                          aCGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               >  1:289155/1‑84 (MQ=255)                                                          aCGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               <  2:289155/84‑1 (MQ=255)                                                                gACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTg                                                                  >  2:149919/1‑139 (MQ=255)                                                                gACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTg                                                                  >  2:715670/1‑139 (MQ=255)                                                                 acacAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAAc                                                                                                                                      <  1:291897/70‑1 (MQ=255)                                                                 acacAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAAc                                                                                                                                      >  2:291897/1‑70 (MQ=255)                                                                 acacAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTgg                                                                 >  1:629832/1‑139 (MQ=255)                                                                 acacAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTgg                                                                 >  1:835504/1‑139 (MQ=255)                                                                     aaaCATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                          >  1:1096837/1‑78 (MQ=255)                                                                     aaaCATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                          <  2:1096837/78‑1 (MQ=255)                                                                       aCATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAg                                                           <  2:835504/139‑1 (MQ=255)                                                                             ggATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGtt                                                                      >  2:914691/1‑122 (MQ=255)                                                                             ggATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGtt                                                                      <  1:914691/122‑1 (MQ=255)                                                                               aTCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCg                                                   <  2:629832/139‑1 (MQ=255)                                                                                 ctgtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGAc                                                                                                >  1:1005711/1‑92 (MQ=255)                                                                                 ctgtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGAc                                                                                                <  2:1005711/92‑1 (MQ=255)                                                                                   gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGa                                                                                                 <  2:1340588/89‑1 (MQ=255)                                                                                   gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGa                                                                                                 >  1:1340588/1‑89 (MQ=255)                                                                                   gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTg                                                     <  2:884713/133‑1 (MQ=255)                                                                                   gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTg                                                     >  1:884713/1‑133 (MQ=255)                                                                                   gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCg                                                   >  1:1412283/1‑135 (MQ=255)                                                                                   gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCg                                                   <  2:1412283/135‑1 (MQ=255)                                                                                            gcgcCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGaa                                      >  1:478330/1‑139 (MQ=255)                                                                                             cgccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGAc                                                                                                <  1:1289805/80‑1 (MQ=255)                                                                                             cgccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGAc                                                                                                >  2:1289805/1‑80 (MQ=255)                                                                                              gccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATg                                    >  2:704578/1‑139 (MQ=255)                                                                                                          ttACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                          <  1:1202033/41‑1 (MQ=255)                                                                                                          ttACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                          >  2:1202033/1‑41 (MQ=255)                                                                                                                            aaGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGa                                                                                                 >  2:1172851/1‑48 (MQ=255)                                                                                                                            aaGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGa                                                                                                 <  1:1172851/48‑1 (MQ=255)                                                                                                                            aaGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                         >  1:1174967/1‑56 (MQ=255)                                                                                                                            aaGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                         <  2:1174967/56‑1 (MQ=255)                                                                                                                            aaGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACttt      >  1:904679/1‑139 (MQ=255)                                                                                                                                aTAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACAGGCTGCACTCTTTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACCAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTTTCGaa  <  1:1297012/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        TACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTTTCGAA  >  NC_000913/1833981‑1834246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑