Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,862,634	A→G	intergenic (‑205/‑137)	msrB ← / → gapA	methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,862,634	0	A	G	100.0%	177.4 / NA	49	intergenic (‑205/‑137)	msrB/gapA	methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/27);  total (22/27)

AATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGAGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTA  >  NC_000913/1862503‑1862743                                                                                                                                    |                                                                                                              aaTTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagt                                                                                                        <  2:114113/139‑1 (MQ=255)       gTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTcg                                                                                                  >  1:591984/1‑139 (MQ=255)         ccTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTcgcg                                                                                                <  2:1322696/139‑1 (MQ=255)                  gATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTa                                                                                       >  2:1127739/1‑139 (MQ=255)                      tcGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                   <  2:905388/139‑1 (MQ=255)                      tcGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                   <  2:591984/139‑1 (MQ=255)                          cgaAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAgg                                                                               >  2:423636/1‑139 (MQ=255)                               tGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                   >  1:1175945/1‑130 (MQ=255)                               tGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                   <  2:1175945/130‑1 (MQ=255)                                   tGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                   <  1:232131/126‑1 (MQ=255)                                   tGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                   >  2:232131/1‑126 (MQ=255)                                        cTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCa                                                                 <  2:511397/139‑1 (MQ=255)                                                tgatgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGa                                                         >  2:1258238/1‑139 (MQ=255)                                                 gatgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagt                                                                                                        >  1:490414/1‑91 (MQ=255)                                                 gatgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagt                                                                                                        <  2:490414/91‑1 (MQ=255)                                                  atgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGaca                                                       >  2:166423/1‑139 (MQ=255)                                                  atgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGaca                                                       >  2:1154714/1‑139 (MQ=255)                                                  atgaAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGaca                                                       <  1:1127739/139‑1 (MQ=255)                                                     aaaaTCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGa                                                    <  2:1342636/139‑1 (MQ=255)                                                                 ttATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                        <  1:423636/139‑1 (MQ=255)                                                                 ttATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACg                                        <  1:1258238/139‑1 (MQ=255)                                                                   aTCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                   >  1:629805/1‑94 (MQ=255)                                                                   aTCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca                                                                                   <  2:629805/94‑1 (MQ=255)                                                                         aTTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcagt                                                                                                    >  2:1125198/1‑71 (MQ=255)                                                                         aTTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGagtcagt                                                                                                    <  1:1125198/71‑1 (MQ=255)                                                                         aTTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCa                                                                 >  2:1422591/1‑106 (MQ=255)                                                                         aTTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCa                                                                 <  1:1422591/106‑1 (MQ=255)                                                                         aTTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTaa                                >  2:541361/1‑139 (MQ=255)                                                                               cTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGtttt                          <  2:992787/139‑1 (MQ=255)                                                                                  tAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTcgcg                                                                                                >  2:1298107/1‑66 (MQ=255)                                                                                  tAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTcgcg                                                                                                <  1:1298107/66‑1 (MQ=255)                                                                                  tAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCtt                                                                                        >  1:619335/1‑74 (MQ=255)                                                                                  tAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCtt                                                                                        <  2:619335/74‑1 (MQ=255)                                                                                               gcCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCa          <  1:166423/139‑1 (MQ=255)                                                                                                 cGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGAc                                         <  2:927147/106‑1 (MQ=255)                                                                                                 cGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGAc                                         >  1:927147/1‑106 (MQ=255)                                                                                                       cATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTa  <  1:541361/139‑1 (MQ=255)                                                                                                             gTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCt                                             <  1:11443/90‑1 (MQ=255)                                                                                                             gTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCt                                             >  2:11443/1‑90 (MQ=255)                                                                                                              tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGc                                                                  <  2:712860/68‑1 (MQ=255)                                                                                                              tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGc                                                                  >  1:712860/1‑68 (MQ=255)                                                                                                              tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTaa                                >  1:37763/1‑102 (MQ=255)                                                                                                              tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTaa                                <  2:37763/102‑1 (MQ=255)                                                                                                              tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAAc        >  2:43772/1‑126 (MQ=255)                                                                                                              tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAAc        <  1:43772/126‑1 (MQ=255)                                                                                                                        aaaGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGAc                                         <  1:1304110/83‑1 (MQ=255)                                                                                                                        aaaGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGAc                                         >  2:1304110/1‑83 (MQ=255)                                                                                                                          aGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAg             >  1:377852/1‑109 (MQ=255)                                                                                                                          aGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAg             <  2:377852/109‑1 (MQ=255)                                                                                                                                    |                                                                                                              AATTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGAGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTA  >  NC_000913/1862503‑1862743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑