Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,078	C→T	*35* (TAG→TAA)	yoaI ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,078	0	C	T	100.0%	149.8 / NA	48	*35* (TAG→TAA)	yoaI	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

CTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCAC  >  NC_000913/1873952‑1874212                                                                                                                               |                                                                                                                                       ctctTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGt                                                                                                                            <  1:818268/139‑1 (MQ=255)    ttCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCg                                                                                                                         >  2:77153/1‑139 (MQ=255)      cGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCA‑‑GCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATaa                                                                                                                     >  1:452876/1‑139 (MQ=255)        aaCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGAGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATaa                                                                                                                     >  2:931960/1‑139 (MQ=255)              ttGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAgaagaa                                                                                                               <  2:582803/139‑1 (MQ=255)                   gaAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                          <  1:77153/139‑1 (MQ=255)                       tCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGAt                                                                                                                       <  1:1381478/122‑1 (MQ=255)                       tCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGAt                                                                                                                       >  2:1381478/1‑122 (MQ=255)                       tCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaaa                                                                                                      >  2:310934/1‑139 (MQ=255)                           cTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAg                                                                                                  <  2:768354/139‑1 (MQ=255)                              cAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTac                                                                                               <  1:310934/139‑1 (MQ=255)                                   cAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCA‑‑GCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCa                                                                                        <  2:452876/139‑1 (MQ=255)                                           cTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCa                                                                                  >  1:1404582/1‑139 (MQ=255)                                                  aaTCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACg                                                                           >  1:1156738/1‑139 (MQ=255)                                                        ttATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCTGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                                        <  2:784984/136‑1 (MQ=255)                                                        ttATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCTGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                                        >  1:784984/1‑136 (MQ=255)                                                        ttATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAgaagaa                                                                                                               >  1:667954/1‑97 (MQ=255)                                                        ttATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAgaagaa                                                                                                               <  2:667954/97‑1 (MQ=255)                                                            tttGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGAt                                                                 <  2:21850/139‑1 (MQ=255)                                                                     cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTac                                                                                               <  1:268186/100‑1 (MQ=255)                                                                     cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTac                                                                                               >  2:268186/1‑100 (MQ=255)                                                                     cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAgtgt                                                        >  2:296136/1‑139 (MQ=255)                                                                       cgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGtct                                                      >  2:746019/1‑139 (MQ=255)                                                                                      ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGt                                                                                                                            <  1:1225952/54‑1 (MQ=255)                                                                                      ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGt                                                                                                                            >  2:1225952/1‑54 (MQ=255)                                                                                      ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt                                       <  2:510847/139‑1 (MQ=255)                                                                                      ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt                                       <  1:296136/139‑1 (MQ=255)                                                                                        gcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCa                                                            >  1:839052/1‑116 (MQ=255)                                                                                        gcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCa                                                            <  2:839052/116‑1 (MQ=255)                                                                                            aaCCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                                        <  2:653824/100‑1 (MQ=255)                                                                                            aaCCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                                        >  1:653824/1‑100 (MQ=255)                                                                                                aGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACATCAATGGGAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGc                             <  1:746019/139‑1 (MQ=255)                                                                                                 gAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCa                            <  1:931960/139‑1 (MQ=255)                                                                                                   aCTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATg                          >  2:694242/1‑139 (MQ=255)                                                                                                    cTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGc                         <  2:1156738/139‑1 (MQ=255)                                                                                                       gTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                  >  1:68790/1‑111 (MQ=255)                                                                                                       gTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                  <  2:68790/111‑1 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGa                                                                       >  2:947367/1‑89 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGa                                                                       <  1:947367/89‑1 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGAc                                                                      <  2:1081661/90‑1 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGAc                                                                      >  1:1081661/1‑90 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt                                       >  1:928529/1‑121 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt                                       <  2:928529/121‑1 (MQ=255)                                                                                                        tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGccccc                     >  1:1355630/1‑139 (MQ=255)                                                                                                         cAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                                        >  2:181084/1‑119 (MQ=255)                                                                                                         cAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                                        <  1:181084/119‑1 (MQ=255)                                                                                                                          cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCa   >  1:319507/1‑139 (MQ=255)                                                                                                                           gTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCAc  <  1:694242/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                       CTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCAC  >  NC_000913/1873952‑1874212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑