Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,798	G→A	*149* (TAG→TAA)  T260I (ACT→ATT)	yeaL → yeaM ←	UPF0756 family putative inner membrane protein putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,798	0	G	A	100.0%	142.3 / NA	45	*149* (TAG→TAA)  T260I (ACT→ATT)	yeaL yeaM	UPF0756 family putative inner membrane protein putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/27);  total (18/27)

TTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAA  >  NC_000913/1874664‑1874930                                                                                                                                       |                                                                                                                                     ttAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTaa                                                                                                                                  <  1:793261/139‑1 (MQ=255)    aTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                                                               <  1:1152438/139‑1 (MQ=255)        gCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                                                           <  1:1240219/139‑1 (MQ=255)        gCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                                                           <  1:1427179/139‑1 (MQ=255)           gccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtc                                                                                                                                >  2:1324991/1‑131 (MQ=255)           gccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtc                                                                                                                                <  1:1324991/131‑1 (MQ=255)                 cGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCgg                                                                                                                  >  1:450146/1‑139 (MQ=255)                         gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                                                               >  2:805482/1‑118 (MQ=255)                         gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                                                               <  1:805482/118‑1 (MQ=255)                         gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                                                          <  1:181621/139‑1 (MQ=255)                          tCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                         <  1:1111548/139‑1 (MQ=255)                               gggCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGccc                                                                                                    <  2:342667/139‑1 (MQ=255)                                    gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                               <  2:1404582/139‑1 (MQ=255)                                               cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAAc                                                                                    >  1:673776/1‑139 (MQ=255)                                                tGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                                   >  1:464090/1‑139 (MQ=255)                                                tGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                                   <  2:229303/139‑1 (MQ=255)                                                tGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                                   >  2:1061978/1‑139 (MQ=255)                                                 gTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACAt                                                                                  <  2:464090/139‑1 (MQ=255)                                                                    ttCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttgtt                                                               <  1:1061978/139‑1 (MQ=255)                                                                    ttCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttgtt                                                               <  2:450146/139‑1 (MQ=255)                                                                             tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTg                                                                                                              <  2:1352585/83‑1 (MQ=255)                                                                             tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTg                                                                                                              >  1:1352585/1‑83 (MQ=255)                                                                             tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATAc                                                      >  1:37634/1‑139 (MQ=255)                                                                               ccGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                                                               <  1:1242029/64‑1 (MQ=255)                                                                               ccGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                                                               >  2:1242029/1‑64 (MQ=255)                                                                                     gggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGt                                              >  1:785110/1‑139 (MQ=255)                                                                                       gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                               <  2:456134/88‑1 (MQ=255)                                                                                       gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                               >  1:456134/1‑88 (MQ=255)                                                                                       gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTAt                                            >  2:134477/1‑139 (MQ=255)                                                                                          gCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAg                                         <  2:785110/139‑1 (MQ=255)                                                                                            ttATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa                                       <  2:673776/139‑1 (MQ=255)                                                                                             tATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttgtt                                                               <  1:743791/114‑1 (MQ=255)                                                                                             tATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttgtt                                                               >  2:743791/1‑114 (MQ=255)                                                                                                     cgGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGcc                                                                                                                >  1:801068/1‑57 (MQ=255)                                                                                                     cgGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGcc                                                                                                                <  2:801068/57‑1 (MQ=255)                                                                                                        gTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                               <  1:1378915/71‑1 (MQ=255)                                                                                                        gTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                               >  2:1378915/1‑71 (MQ=255)                                                                                                                  gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATAcccc                                                   <  2:572126/105‑1 (MQ=255)                                                                                                                  gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATAcccc                                                   >  1:572126/1‑105 (MQ=255)                                                                                                                       ttGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                         >  1:1194790/1‑46 (MQ=255)                                                                                                                       ttGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                         <  2:1194790/46‑1 (MQ=255)                                                                                                                                aaCAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                               >  1:975128/1‑47 (MQ=255)                                                                                                                                aaCAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                               <  2:975128/47‑1 (MQ=255)                                                                                                                                 aCAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCGGGCTTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGaa  <  1:134477/139‑1 (MQ=255)                                                                                                                                 aCAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGaa  <  2:37634/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     TTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAA  >  NC_000913/1874664‑1874930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑