Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,926,096	G→A	*219* (TAG→TAA)	yobB →	C‑N hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,096	0	G	A	100.0%	206.0 / NA	66	*219* (TAG→TAA)	yobB	C‑N hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/33);  total (33/33)

GGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925961‑1926229                                                                                                                                        |                                                                                                                                      ggCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCt                                                                                                                                    >  2:869584/1‑139 (MQ=255)          ccGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:970515/139‑1 (MQ=255)              ggTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       <  1:1145089/139‑1 (MQ=255)                        cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             <  2:977590/139‑1 (MQ=255)                         aCTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGt                                                                                                            >  2:1114047/1‑139 (MQ=255)                              gggATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       <  1:869584/139‑1 (MQ=255)                                          gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGc                                                                                                                 <  2:149442/117‑1 (MQ=255)                                          gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGc                                                                                                                 >  1:149442/1‑117 (MQ=255)                                              tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       <  1:994087/107‑1 (MQ=255)                                              tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa                                                                                                                       >  2:994087/1‑107 (MQ=255)                                              tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCACTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGaaa                                                                                       >  1:874178/1‑139 (MQ=255)                                                              gATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCaggg                                                                       <  1:315718/139‑1 (MQ=255)                                                               aTCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCaggga                                                                      >  2:636774/1‑139 (MQ=255)                                                                ttgttgTTTATTAGTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTCCGCAAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                     <  2:254575/134‑1 (MQ=255)                                                                tCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCGAAGCTATTTCGGACTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCTGATTGCagggag                                                                     >  2:977654/1‑139 (MQ=255)                                                                     gTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGGTTGCAGGGAGGGATc                                                                >  1:334464/1‑139 (MQ=255)                                                                         attattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTg                                                            >  1:242608/1‑139 (MQ=255)                                                                          ttattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTg                                                                                                          >  2:775114/1‑92 (MQ=255)                                                                          ttattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTg                                                                                                          <  1:775114/92‑1 (MQ=255)                                                                              tGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGt                                                              >  2:537568/1‑132 (MQ=255)                                                                              tGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGt                                                              <  1:537568/132‑1 (MQ=255)                                                                                ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGTATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGc                                                     <  1:636774/139‑1 (MQ=255)                                                                                ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCg                                                                                 >  2:979559/1‑111 (MQ=255)                                                                                ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCg                                                                                 <  1:979559/111‑1 (MQ=255)                                                                                ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGc                                                     <  1:1114047/139‑1 (MQ=255)                                                                                ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGc                                                     <  2:242608/139‑1 (MQ=255)                                                                                ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGc                                                     >  1:860204/1‑139 (MQ=255)                                                                                  aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCgg                                                                                >  1:1025672/1‑110 (MQ=255)                                                                                  aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCgg                                                                                <  2:1025672/110‑1 (MQ=255)                                                                                  aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   >  1:838957/1‑139 (MQ=255)                                                                                  aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   >  1:456852/1‑139 (MQ=255)                                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt                                                                                                                                <  1:1401188/57‑1 (MQ=255)                                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt                                                                                                                                >  2:1401188/1‑57 (MQ=255)                                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  >  1:1394020/1‑71 (MQ=255)                                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  <  2:1394020/71‑1 (MQ=255)                                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             <  1:168268/76‑1 (MQ=255)                                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             >  2:168268/1‑76 (MQ=255)                                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   >  2:1416084/1‑134 (MQ=255)                                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   <  1:1416084/134‑1 (MQ=255)                                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGtt                                              >  2:627996/1‑139 (MQ=255)                                                                                        tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  <  2:97714/70‑1 (MQ=255)                                                                                        tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  >  1:97714/1‑70 (MQ=255)                                                                                                    acaGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGc                                                                                                                            >  1:733525/1‑48 (MQ=255)                                                                                                    acaGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGc                                                                                                                            <  2:733525/48‑1 (MQ=255)                                                                                                      aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                     <  2:1261122/101‑1 (MQ=255)                                                                                                      aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                     >  1:1261122/1‑101 (MQ=255)                                                                                                      aGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGa                               >  2:670364/1‑139 (MQ=255)                                                                                                        ggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGc                                                                                                                            >  2:149978/1‑44 (MQ=255)                                                                                                        ggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGc                                                                                                                            <  1:149978/44‑1 (MQ=255)                                                                                                        ggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa                                                                          >  2:404206/1‑94 (MQ=255)                                                                                                        ggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa                                                                          <  1:404206/94‑1 (MQ=255)                                                                                                           tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggaggg                                                                   <  1:299722/98‑1 (MQ=255)                                                                                                           tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggaggg                                                                   >  2:299722/1‑98 (MQ=255)                                                                                                            ggCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGc                                                                                                                     <  1:238191/47‑1 (MQ=255)                                                                                                            ggCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGc                                                                                                                     >  2:238191/1‑47 (MQ=255)                                                                                                             gaaaGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCAATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTgag                                                          <  2:487375/103‑1 (MQ=255)                                                                                                             gCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTgag                                                          >  1:487375/1‑105 (MQ=255)                                                                                                               aaGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTc                      <  1:670364/139‑1 (MQ=255)                                                                                                                     atatCACTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAcccc                <  2:874178/139‑1 (MQ=255)                                                                                                                          aTTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAg           <  2:860204/139‑1 (MQ=255)                                                                                                                               aTTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                     <  2:315550/76‑1 (MQ=255)                                                                                                                               aTTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                     >  1:315550/1‑76 (MQ=255)                                                                                                                                   cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCTCATTATCGATACAGACACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt  <  1:977654/139‑1 (MQ=255)                                                                                                                                   cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   <  2:361074/90‑1 (MQ=255)                                                                                                                                   cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   >  1:361074/1‑90 (MQ=255)                                                                                                                                   cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt  >  2:1362729/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      GGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925961‑1926229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑