Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,046,399	C→T	P488S (CCG→TCG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,046,399	0	C	T	100.0%	124.1 / NA	39	P488S (CCG→TCG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/19);  total (20/19)

CGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAG  >  NC_000913/2046289‑2046533                                                                                                               |                                                                                                                                       cgtcgCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGa                                                                                                            >  2:486654/1‑139 (MQ=255)                aaTATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTg                                                                                             <  2:1243118/139‑1 (MQ=255)                  tatGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGc                                                                                           <  1:486654/139‑1 (MQ=255)                  tatGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGc                                                                                           <  1:633089/139‑1 (MQ=255)                   atGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTAc                                                                                                                                >  1:1140867/1‑101 (MQ=255)                   atGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTAc                                                                                                                                <  2:1140867/101‑1 (MQ=255)                          gAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGc                                                                                           >  1:1046775/1‑131 (MQ=255)                          gAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGc                                                                                           <  2:1046775/131‑1 (MQ=255)                           aaaGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTgg                                                                                            <  2:762792/129‑1 (MQ=255)                           aaaGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTgg                                                                                            >  1:762792/1‑129 (MQ=255)                                   taACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCAccgcc                                                                          >  2:715932/1‑139 (MQ=255)                                     aCGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGaa                                                                                                           >  2:925151/1‑104 (MQ=255)                                     aCGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGaa                                                                                                           <  1:925151/104‑1 (MQ=255)                                       gTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAg                                                                      <  1:1148549/139‑1 (MQ=255)                                          gAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGt                                                                                 >  1:1094771/1‑125 (MQ=255)                                          gAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGt                                                                                 <  2:1094771/125‑1 (MQ=255)                                                 ccGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGtt                                                                                                                           <  1:810330/76‑1 (MQ=255)                                                 ccGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGtt                                                                                                                           >  2:810330/1‑76 (MQ=255)                                                  cGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGc                                                           >  2:1319818/1‑139 (MQ=255)                                                         gAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGt                                                    >  2:464255/1‑139 (MQ=255)                                                               gCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATc                                              <  1:715932/139‑1 (MQ=255)                                                                   gTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGa                                          >  1:533418/1‑139 (MQ=255)                                                                          aGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGc                                   <  1:464255/139‑1 (MQ=255)                                                                           gTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCa                                  <  2:533418/139‑1 (MQ=255)                                                                                cacaACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCAt                                 >  1:291431/1‑135 (MQ=255)                                                                                cacaACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCAt                                 <  2:291431/135‑1 (MQ=255)                                                                                        gTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCa                     >  2:492242/1‑139 (MQ=255)                                                                                               tatTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGa                                                                                                      >  2:400651/1‑51 (MQ=255)                                                                                               tatTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGa                                                                                                      <  1:400651/51‑1 (MQ=255)                                                                                                 ttCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGaa                                                                                                           <  1:955505/44‑1 (MQ=255)                                                                                                 ttCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTAc            >  2:836900/1‑139 (MQ=255)                                                                                                 ttCTGGTTACCCTGTAGGCTTACCGGTTCACCAGTACGCCAGaa                                                                                                           >  2:955505/1‑44 (MQ=255)                                                                                                  tCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAg                                                                      >  2:261179/1‑80 (MQ=255)                                                                                                  tCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAg                                                                      <  1:261179/80‑1 (MQ=255)                                                                                                      gTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATggtggt                           >  2:695143/1‑119 (MQ=255)                                                                                                      gTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATggtggt                           <  1:695143/119‑1 (MQ=255)                                                                                                         aCCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCa                 <  2:134571/126‑1 (MQ=255)                                                                                                         aCCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCa                 >  1:134571/1‑126 (MQ=255)                                                                                                           ccTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCGAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAg  >  2:1027363/1‑139 (MQ=255)                                                                                                               |                                                                                                                                       CGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAG  >  NC_000913/2046289‑2046533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑