Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,046,607	T→C	I557T (ATT→ACT)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,046,607	0	T	C	100.0%	179.4 / NA	50	I557T (ATT→ACT)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

GGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAG  >  NC_000913/2046474‑2046738                                                                                                                                      |                                                                                                                                    ggCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTgcgc                                                                                                                                >  1:302551/1‑139 (MQ=255)  gCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCa                                                                                                                               <  2:531588/139‑1 (MQ=255)  gCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCa                                                                                                                               >  2:358945/1‑139 (MQ=255)      tttGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCatgat                                                                                                                           >  1:386736/1‑139 (MQ=255)              aTCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAgg                                                                                                                   >  2:877423/1‑139 (MQ=255)                           cATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTc                                                                                                      >  2:143000/1‑139 (MQ=255)                                           gCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGa                                                                                      <  1:358945/139‑1 (MQ=255)                                               cTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGAcgcg                                                                                  >  1:1079363/1‑139 (MQ=255)                                                       aGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAGCGCGGATGCCCATTCAACCGCCACATTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCAGGCTGGTTCTCTCGACGCGTCACGAAg                                                                          <  2:63876/139‑1 (MQ=255)                                                       aGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAg                                                                          <  1:1027363/139‑1 (MQ=255)                                                          cAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGgtg                                                                       <  1:877423/139‑1 (MQ=255)                                                            gAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCatg                                                                                                                             >  2:289790/1‑83 (MQ=255)                                                            gAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCatg                                                                                                                             <  1:289790/83‑1 (MQ=255)                                                            gAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATgca                                                                                                                        <  1:296913/88‑1 (MQ=255)                                                            gAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATgca                                                                                                                        >  2:296913/1‑88 (MQ=255)                                                            gAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGtt                                                                     >  1:573747/1‑139 (MQ=255)                                                              aaGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCa                                                                   <  1:46602/139‑1 (MQ=255)                                                                            tCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCttt                                                     <  1:143000/139‑1 (MQ=255)                                                                                  aGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCGTGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACt                                               >  1:139833/1‑139 (MQ=255)                                                                                    tACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTc                                                    >  1:1289461/1‑132 (MQ=255)                                                                                    tACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTc                                                    <  2:1289461/132‑1 (MQ=255)                                                                                    tACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTgg                                             >  1:1315743/1‑139 (MQ=255)                                                                                      cccAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGAcg                                                                                    <  1:1293129/98‑1 (MQ=255)                                                                                      cccAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGAcg                                                                                    >  2:1293129/1‑98 (MQ=255)                                                                                      cccAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGa                                                                            <  1:15652/106‑1 (MQ=255)                                                                                      cccAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGa                                                                            >  2:15652/1‑106 (MQ=255)                                                                                      cccAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGaa                                           >  1:117770/1‑139 (MQ=255)                                                                                        cAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAg                                         <  2:1079363/139‑1 (MQ=255)                                                                                              ttGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAg                                                                          >  1:1199715/1‑100 (MQ=255)                                                                                              ttGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAg                                                                          <  2:1199715/100‑1 (MQ=255)                                                                                                 aaCGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAg                                                                          >  2:1202020/1‑97 (MQ=255)                                                                                                 aaCGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAg                                                                          <  1:1202020/97‑1 (MQ=255)                                                                                                 aaCGCGGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTg                                >  2:713140/1‑139 (MQ=255)                                                                                                     cgGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCGTGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACgg                            <  2:139833/139‑1 (MQ=255)                                                                                                     cgGATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACgg                            <  2:536499/139‑1 (MQ=255)                                                                                                       gATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTaa                                                                                                                <  2:1351998/53‑1 (MQ=255)                                                                                                       gATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTaa                                                                                                                >  1:1351998/1‑53 (MQ=255)                                                                                                       gATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAgg                                                                         >  2:1109290/1‑92 (MQ=255)                                                                                                       gATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAgg                                                                         <  1:1109290/92‑1 (MQ=255)                                                                                                       gATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCt                                                   >  1:1345446/1‑114 (MQ=255)                                                                                                       gATTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCt                                                   <  2:1345446/114‑1 (MQ=255)                                                                                                        aTTCCCATTCAACCGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAg                         <  2:386736/139‑1 (MQ=255)                                                                                                                    ccGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGa                                                                            <  2:525003/76‑1 (MQ=255)                                                                                                                    ccGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGa                                                                            >  1:525003/1‑76 (MQ=255)                                                                                                                    ccGCCACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGAt             >  2:434451/1‑139 (MQ=255)                                                                                                                       ccACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGtt                                                                     >  2:528942/1‑80 (MQ=255)                                                                                                                       ccACACTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGtt                                                                     <  1:528942/80‑1 (MQ=255)                                                                                                                            cTGGCTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGATGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACca     <  2:302551/139‑1 (MQ=255)                                                                                                                            cTGACTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACca     <  1:713140/139‑1 (MQ=255)                                                                                                                               aCTTTTACTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAg  >  1:922001/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                    GGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAG  >  NC_000913/2046474‑2046738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑