Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,072,980 T→C L481P (CTG→CCG)  flu → CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,072,9800TC100.0% 404.8 / NA 110L481P (CTG→CCG) fluCP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/56);  total (54/56)

Only 100 of 110 total aligned reads displayed.

TGGCGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAG  >  NC_000913/2072849‑2073109
                                                                                                                                   |                                                                                                                                 
tGGCGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGa                                                                                                                            <  2:1214283/139‑1 (MQ=255)
   cGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGc                                                                                                                         <  1:1173088/139‑1 (MQ=255)
     gACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGctc                                                                                                                       >  1:241966/1‑139 (MQ=255)
       cTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGctctc                                                                                                                     <  2:241966/139‑1 (MQ=255)
          ttCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCa                                                                                                                  <  2:443266/139‑1 (MQ=255)
          ttCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCa                                                                                                                  <  2:1224823/139‑1 (MQ=255)
          ttCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCa                                                                                                                  >  1:491332/1‑139 (MQ=255)
          ttCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGCCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGc                                                                                                                         <  2:868093/132‑1 (MQ=255)
          ttCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGCCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGc                                                                                                                         >  1:868093/1‑132 (MQ=255)
            cACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCAcc                                                                                                                >  1:998506/1‑139 (MQ=255)
                    ggggCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAgg                                                                                                                          >  2:71830/1‑121 (MQ=255)
                    ggggCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAgg                                                                                                                          <  1:71830/121‑1 (MQ=255)
                    ggggCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACgg                                                                                                        >  1:1146513/1‑139 (MQ=255)
                    ggggCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACgg                                                                                                        >  2:1186670/1‑139 (MQ=255)
                    ggggCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGGGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACgg                                                                                                        >  1:308194/1‑139 (MQ=255)
                              acTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGaa                                                                                              <  2:1425596/139‑1 (MQ=255)
                                tGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACACTGACCATACGTGAAGGCGATGCACTCCCGCAGGGAGGct                                                                                                                        >  2:873413/1‑111 (MQ=255)
                                 ggCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGaaaaa                                                                                           >  1:750502/1‑139 (MQ=255)
                                 ggCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGaaaaa                                                                                           >  2:607610/1‑139 (MQ=255)
                                     ggCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGa                                                                                                                            <  2:404284/102‑1 (MQ=255)
                                     ggCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGa                                                                                                                            >  1:404284/1‑102 (MQ=255)
                                        accaccacGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGaa                                                                                    <  1:1186670/139‑1 (MQ=255)
                                          caccacGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGctct                                                                                                                      <  2:362487/103‑1 (MQ=255)
                                          caccacGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGctct                                                                                                                      >  1:362487/1‑103 (MQ=255)
                                          caccacGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACg                                                                                                         >  1:1010427/1‑116 (MQ=255)
                                          caccacGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGt                                                                                  >  1:789200/1‑139 (MQ=255)
                                          caccacGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCATGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACg                                                                                                         <  2:1010427/116‑1 (MQ=255)
                                                gCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCAGCCGTGAAGGCGATGCACTCCCGCAGGCAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATGAGGAAGTGGcacc                                                                            <  2:644792/139‑2 (MQ=255)
                                                    aaTAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCAcactca                                                                        <  2:491332/139‑1 (MQ=255)
                                                         cGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTc                                                                   <  2:965429/139‑1 (MQ=255)
                                                           gcgcCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAg                                                                 >  2:939239/1‑139 (MQ=255)
                                                            cgcCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGctct                                                                                                                      >  2:86425/1‑85 (MQ=255)
                                                            cgcCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGctct                                                                                                                      <  1:86425/85‑1 (MQ=255)
                                                            cgcCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCa                                                                                                                  >  1:904122/1‑89 (MQ=255)
                                                            cgcCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCa                                                                                                                  <  2:904122/89‑1 (MQ=255)
                                                            cgcCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCACTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGc                                                                >  2:46124/1‑139 (MQ=255)
                                                             gcCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCa                                                               <  2:1146513/139‑1 (MQ=255)
                                                              ccATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCaa                                                              >  1:564320/1‑139 (MQ=255)
                                                              ccATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCaa                                                              >  1:624162/1‑139 (MQ=255)
                                                                        ccTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTc                                                    <  2:382923/139‑1 (MQ=255)
                                                                           ttCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAggg                                                                                                                             <  1:926810/63‑1 (MQ=255)
                                                                           ttCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCAcc                                                 >  2:234098/1‑139 (MQ=255)
                                                                           ttCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCAcc                                                 >  1:510818/1‑139 (MQ=255)
                                                                           ttCCGGGAAGACGGTGAACAACGATACCATGACCATCCGTGAAGGCGATACACTCCCGCAggg                                                                                                                             >  2:926810/1‑63 (MQ=255)
                                                                           ttCCGGGAAGACGGAGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCAcc                                                 >  2:807158/1‑139 (MQ=255)
                                                                            tCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGaa                                                                                    >  1:397811/1‑103 (MQ=255)
                                                                            tCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGt                                                                                  <  2:1325284/105‑1 (MQ=255)
                                                                            tCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGt                                                                                  >  1:1325284/1‑105 (MQ=255)
                                                                            tCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCAccc                                                >  2:1358967/1‑139 (MQ=255)
                                                                            tCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCAccc                                                >  2:398576/1‑139 (MQ=255)
                                                                             ccGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCAcactcact                                                                      <  2:549365/116‑1 (MQ=255)
                                                                             ccGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCAcactcact                                                                      >  1:549365/1‑116 (MQ=255)
                                                                                ggAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGaa                                            <  1:1300945/139‑1 (MQ=255)
                                                                                ggAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGaa                                            >  2:1300945/1‑139 (MQ=255)
                                                                                     aCGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCg                                       <  2:177266/139‑1 (MQ=255)
                                                                                      cGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAgg                                                                                      >  1:1413337/1‑91 (MQ=255)
                                                                                      cGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGt                                      <  2:750502/139‑1 (MQ=255)
                                                                                      cGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGt                                      <  1:939239/139‑1 (MQ=255)
                                                                                      cGGTGAACAACGATACCCTGACCATCAGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAATGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGt                                      >  2:604521/1‑139 (MQ=255)
                                                                                       ggagAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTc                                     <  1:807158/136‑1 (MQ=255)
                                                                                           aacaacGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCa                                                                                                                  <  2:1352302/58‑1 (MQ=255)
                                                                                           aacaacGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCa                                                                                                                  >  1:1352302/1‑58 (MQ=255)
                                                                                            acaacGATACCCTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCt                                <  2:510818/139‑1 (MQ=255)
                                                                                              aacGATACCCTGACCATCGGTGAAGGCGATGCACTCCCGCAGGGAg                                                                                                                           >  2:728826/1‑46 (MQ=38)
                                                                                              aacGATACCCTGACCATCGGTGAAGGCGATGCACTCCCGCAGGGAg                                                                                                                           <  1:728826/46‑1 (MQ=38)
                                                                                                     cccTGACCATCCGTGAATGCGATGCATTCCCGCAGGTAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAgg                       <  2:308194/139‑1 (MQ=255)
                                                                                                     cccTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACgg                                                                                                        >  1:457192/1‑58 (MQ=255)
                                                                                                     cccTGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACgg                                                                                                        <  2:457192/58‑1 (MQ=255)
                                                                                                        tGACCATCCGTGAAGGCGATGCACTCCCGCAGGGCGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAacac                                                           <  2:976562/100‑1 (MQ=255)
                                                                                                        tGACCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAacac                                                           >  1:976562/1‑100 (MQ=255)
                                                                                                          aCCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGct                                                                                                                        <  1:624377/37‑1 (MQ=255)
                                                                                                          aCCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGct                                                                                                                        >  2:624377/1‑37 (MQ=255)
                                                                                                          aCCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGaa                                                                                    <  2:398790/73‑1 (MQ=255)
                                                                                                          aCCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGaa                                                                                    >  1:398790/1‑73 (MQ=255)
                                                                                                          aCCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgc                  >  1:1227689/1‑139 (MQ=255)
                                                                                                          aCCATCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgc                  >  1:87771/1‑139 (MQ=255)
                                                                                                             aTCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgctga               <  2:1397608/139‑1 (MQ=255)
                                                                                                              tCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCa                                                                                        <  2:374461/65‑1 (MQ=255)
                                                                                                              tCCGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCa                                                                                        >  1:374461/1‑65 (MQ=255)
                                                                                                               ccGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGt                                      >  2:29518/1‑114 (MQ=255)
                                                                                                               ccGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGt                                      <  1:29518/114‑1 (MQ=255)
                                                                                                               ccGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgctgacg             >  1:29456/1‑139 (MQ=255)
                                                                                                               ccGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgctgacg             <  1:46124/139‑1 (MQ=255)
                                                                                                                cGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTc                                     <  1:1196390/114‑1 (MQ=255)
                                                                                                                cGTGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTc                                     >  2:1196390/1‑114 (MQ=255)
                                                                                                                  tGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgctgacgctg          <  2:671440/139‑1 (MQ=255)
                                                                                                                  tGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgctgacgctg          <  2:900479/139‑1 (MQ=255)
                                                                                                                  tGAAGGCGATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgctgacgctg          <  1:234098/139‑1 (MQ=255)
                                                                                                                         gATGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGaa                                                                                    <  2:572656/58‑1 (MQ=255)
                                                                                                                          aTGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGa                                                                                               >  1:1222721/1‑46 (MQ=255)
                                                                                                                          aTGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGa                                                                                               <  2:1222721/46‑1 (MQ=255)
                                                                                                                          aTGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACATTCACTGTCAGCAAcacca                                                         <  1:943814/84‑1 (MQ=255)
                                                                                                                          aTGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTc                                                    >  1:552956/1‑89 (MQ=255)
                                                                                                                          aTGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgctga               <  1:218125/126‑1 (MQ=255)
                                                                                                                          aTGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgctga               >  2:218125/1‑126 (MQ=255)
                                                                                                                          aTGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAg  >  2:1070327/1‑139 (MQ=255)
                                                                                                                          aTGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGCAAGTGGCACACTCACTGTCAGCAAcacca                                                         >  2:943814/1‑84 (MQ=255)
                                                                                                                           tGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTgg                                                                                <  1:480731/60‑1 (MQ=255)
                                                                                                                           tGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTgg                                                                                >  2:480731/1‑60 (MQ=255)
                                                                                                                           tGCACTCCCGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACcaca                                                       <  1:827622/85‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
TGGCGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAG  >  NC_000913/2072849‑2073109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑