Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,494	C→T	V6I (GTC→ATC)  *197* (TAG→TAA)	wbbK ← wbbJ ←	lipopolysaccharide biosynthesis protein putative lipopolysaccharide biosynthesis O‑acetyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,494	0	C	T	100.0%	175.3 / NA	55	V6I (GTC→ATC)  *197* (TAG→TAA)	wbbK wbbJ	lipopolysaccharide biosynthesis protein putative lipopolysaccharide biosynthesis O‑acetyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/26);  total (29/26)

TTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCAT  >  NC_000913/2104375‑2104621                                                                                                                        |                                                                                                                                tttAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAtt                                                                                                              >  2:546136/1‑139 (MQ=255)     gCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGt                                                                                                          <  1:102479/139‑1 (MQ=255)         aaTGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCa                                                                                                      <  1:546136/139‑1 (MQ=255)          aTGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                                     <  1:933510/139‑1 (MQ=255)           tGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATg                                                                                                    >  1:585354/1‑139 (MQ=255)                   tGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCa                                                                                                      <  1:1006240/129‑1 (MQ=255)                   tGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCa                                                                                                      >  2:1006240/1‑129 (MQ=255)                   tGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTAtt                                                                                            >  1:520875/1‑139 (MQ=255)                       aTAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCa                                                                                                                   <  1:929031/112‑1 (MQ=255)                       aTAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCa                                                                                                                   >  2:929031/1‑112 (MQ=255)                                     ttCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTg                                                                          >  1:264234/1‑139 (MQ=255)                                      tCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTgg                                                                         <  2:919427/139‑1 (MQ=255)                                                  gTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTa                                                                                              >  1:141560/1‑106 (MQ=255)                                                  gTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTa                                                                                              <  2:141560/106‑1 (MQ=255)                                                  gTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCAATACTTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAAt                                                             >  2:1048060/1‑139 (MQ=255)                                                     gctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCttt                                                                                                                         >  1:316937/1‑76 (MQ=255)                                                     gctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCttt                                                                                                                         <  2:316937/76‑1 (MQ=255)                                                      ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCt                                                                                                                           >  1:84905/1‑73 (MQ=255)                                                      ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCt                                                                                                                           <  2:84905/73‑1 (MQ=255)                                                      ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtct                                                                                                        <  2:1325588/92‑1 (MQ=255)                                                      ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtct                                                                                                        >  1:1325588/1‑92 (MQ=255)                                                      ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACa                                                         >  1:483621/1‑139 (MQ=255)                                                      ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACa                                                         >  2:1049088/1‑139 (MQ=255)                                                      ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTGCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACa                                                         >  2:1024309/1‑139 (MQ=255)                                                       tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTgg                                                                         <  2:142617/122‑1 (MQ=255)                                                       tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTgg                                                                         >  1:142617/1‑122 (MQ=255)                                                       tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAg                                                        >  1:315283/1‑139 (MQ=255)                                                        gcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTccc                                                                                                                    <  1:922992/78‑1 (MQ=255)                                                        gcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTccc                                                                                                                    >  2:922992/1‑78 (MQ=255)                                                         ccAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtc                                                                                                         <  1:1200606/88‑1 (MQ=255)                                                         ccAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtc                                                                                                         >  2:1200606/1‑88 (MQ=255)                                                         ccAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTgg                                                                         <  1:1388373/120‑1 (MQ=255)                                                         ccAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTgg                                                                         >  2:1388373/1‑120 (MQ=255)                                                                   tttCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGa                                                           <  1:157900/124‑1 (MQ=255)                                                                   tttCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGa                                                           >  2:157900/1‑124 (MQ=255)                                                                                aaTGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTaac                               <  2:585354/139‑1 (MQ=255)                                                                                     aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCa                                                                                                      >  2:947399/1‑63 (MQ=255)                                                                                     aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCa                                                                                                      <  1:947399/63‑1 (MQ=255)                                                                                      ccGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTa                                                                                              <  1:685724/70‑1 (MQ=255)                                                                                      ccGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTa                                                                                              >  2:685724/1‑70 (MQ=255)                                                                                       cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACa                                                         >  2:263790/1‑106 (MQ=255)                                                                                       cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACa                                                         <  1:263790/106‑1 (MQ=255)                                                                                          cAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTcc                                                                                                                     <  2:1245757/43‑1 (MQ=255)                                                                                          cAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTcc                                                                                                                     >  1:1245757/1‑43 (MQ=255)                                                                                            gTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                     >  2:370317/1‑89 (MQ=255)                                                                                            gTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                     <  1:370317/89‑1 (MQ=255)                                                                                             tGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGgcg                  <  2:264234/139‑1 (MQ=255)                                                                                                 aaaaTTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTTTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGa              <  2:1017587/139‑1 (MQ=255)                                                                                                     ttGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTAc          <  1:1049088/139‑1 (MQ=255)                                                                                                        aCCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGAc       >  2:561713/1‑139 (MQ=255)                                                                                                         ccGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGgcg                  <  2:1236032/127‑1 (MQ=255)                                                                                                         ccGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGgcg                  >  1:1236032/1‑127 (MQ=255)                                                                                                         ccGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACt      >  1:198608/1‑139 (MQ=255)                                                                                                          cGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTc     >  1:501165/1‑139 (MQ=255)                                                                                                             aGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCAt  <  1:1024309/139‑1 (MQ=255)                                                                                                                        |                                                                                                                                TTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCAT  >  NC_000913/2104375‑2104621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑