Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,019	T→C	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA → arnD →	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,019	0	T	C	100.0%	182.4 / NA	50	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA arnD	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

TAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGC  >  NC_000913/2369885‑2370140                                                                                                                                       |                                                                                                                          tAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGCAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                        <  2:975307/138‑1 (MQ=255) tAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                        >  1:975307/1‑138 (MQ=255)  agagCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCa                                                                                                                      >  2:1283234/1‑139 (MQ=255)     gCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAg                                                                                                                   <  2:332350/139‑1 (MQ=255)      cATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGt                                                                                                                  <  2:402089/139‑1 (MQ=255)                  aGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                        >  2:43378/1‑121 (MQ=255)                  aGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                        <  1:43378/121‑1 (MQ=255)                                        gCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                <  1:1301538/139‑1 (MQ=255)                                         ccTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                              <  2:235947/108‑1 (MQ=255)                                         ccTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                              >  1:235947/1‑108 (MQ=255)                                                         aaaaTTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTg                                                                                                    <  2:651119/102‑1 (MQ=255)                                                         aaaaTTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTg                                                                                                    >  1:651119/1‑102 (MQ=255)                                                         aaaaTTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGcc                                                               >  2:102449/1‑139 (MQ=255)                                                         aaaaTTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGcc                                                               >  1:589434/1‑139 (MQ=255)                                                             ttGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGt                                                           <  1:102449/139‑1 (MQ=255)                                                             ttGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGt                                                           <  1:1283234/139‑1 (MQ=255)                                                                atatGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTctg                                                        >  2:295708/1‑139 (MQ=255)                                                                     cAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGa                                                   <  1:295708/139‑1 (MQ=255)                                                                        gAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                        >  1:43439/1‑67 (MQ=255)                                                                        gAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                        <  2:43439/67‑1 (MQ=255)                                                                        gAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                <  1:1431787/107‑1 (MQ=255)                                                                        gAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                >  2:1431787/1‑107 (MQ=255)                                                                        gAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAAt                                                >  2:393507/1‑139 (MQ=255)                                                                              aTCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAg                                          >  2:952816/1‑139 (MQ=255)                                                                               tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                        <  1:254147/60‑1 (MQ=255)                                                                               tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                        >  2:254147/1‑60 (MQ=255)                                                                               tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                         >  2:1405345/1‑139 (MQ=255)                                                                                           tGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAgg                                                                     >  2:21452/1‑99 (MQ=255)                                                                                           tGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAgg                                                                     <  1:21452/99‑1 (MQ=255)                                                                                            ggATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTc                            >  1:245417/1‑139 (MQ=255)                                                                                             gATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                     >  2:601864/1‑65 (MQ=255)                                                                                             gATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                     <  1:601864/65‑1 (MQ=255)                                                                                                        gcgcACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                       >  1:34991/1‑116 (MQ=255)                                                                                                        gcgcACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                       <  2:34991/116‑1 (MQ=255)                                                                                                             ccGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                <  1:101656/70‑1 (MQ=255)                                                                                                             ccGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                >  2:101656/1‑70 (MQ=255)                                                                                                             ccGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                       <  1:1382436/111‑1 (MQ=255)                                                                                                             ccGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                       >  2:1382436/1‑111 (MQ=255)                                                                                                              cGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCa          >  1:986883/1‑139 (MQ=255)                                                                                                                ttGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGt                                                                                   <  1:891733/64‑1 (MQ=255)                                                                                                                ttGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGt                                                                                   >  2:891733/1‑64 (MQ=255)                                                                                                                 tGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCg       <  1:1247838/139‑1 (MQ=255)                                                                                                                      ttACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGc  <  2:245417/139‑1 (MQ=255)                                                                                                                      ttACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGc  <  1:459108/139‑1 (MQ=255)                                                                                                                        aCGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCcgc                                                             <  1:849113/78‑1 (MQ=255)                                                                                                                        aCGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCcgc                                                             >  2:849113/1‑78 (MQ=255)                                                                                                                             tAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGc  >  2:1127467/1‑132 (MQ=255)                                                                                                                             tAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGc  <  1:1127467/132‑1 (MQ=255)                                                                                                                              aaaCCATCACGACCAAAGTAGGCTTACGCATTGATGt                                                                                                <  2:270714/37‑1 (MQ=255)                                                                                                                              aaaCCATCACGACCAAAGTAGGCTTACGCATTGATGt                                                                                                >  1:270714/1‑37 (MQ=255)                                                                                                                                       |                                                                                                                          TAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGC  >  NC_000913/2369885‑2370140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑