Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,475,251 T→C Y90H (TAT→CAT)  yfdR → CPS‑53 (KpLE1) prophage, conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,475,2510TC100.0% 257.8 / NA 72Y90H (TAT→CAT) yfdRCPS‑53 (KpLE1) prophage, conserved protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/37);  total (35/37)

TCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCG  >  NC_000913/2475123‑2475374
                                                                                                                                |                                                                                                                           
tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGAt                                                                                                                   >  1:80416/1‑139 (MQ=255)
tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGAt                                                                                                                   >  1:1398641/1‑139 (MQ=255)
tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGAt                                                                                                                   >  1:293305/1‑139 (MQ=255)
tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGAt                                                                                                                   >  2:83440/1‑139 (MQ=255)
  gCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATgg                                                                                                                 >  2:154954/1‑139 (MQ=255)
   cccAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGga                                                                                                                >  2:1383517/1‑139 (MQ=255)
      aaCATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGgaaga                                                                                                             <  1:441992/139‑1 (MQ=255)
            cGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAAt                                                                                                       <  2:80416/139‑1 (MQ=255)
               tGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGa                                                                                                    <  1:1053555/139‑1 (MQ=255)
                     gCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGt                                                                                              <  1:83440/139‑1 (MQ=255)
                     gCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGt                                                                                              <  1:154954/139‑1 (MQ=255)
                             cTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTg                                                                                      <  1:196380/139‑1 (MQ=255)
                                          aaTTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt                                                                         <  2:293305/139‑1 (MQ=255)
                                          aaTTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt                                                                         <  1:698008/139‑1 (MQ=255)
                                            tttGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAc                                                                       <  1:1270759/139‑1 (MQ=255)
                                               gCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcctc                                                                    <  2:441774/139‑1 (MQ=255)
                                                   ttGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAc                                                                       <  1:1083337/132‑1 (MQ=255)
                                                   ttGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAc                                                                       >  2:1083337/1‑132 (MQ=255)
                                                                gCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGGAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCa                                                   <  2:1398641/139‑1 (MQ=255)
                                                                 cATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAg                                                  <  1:667545/139‑1 (MQ=255)
                                                                      tgCAACAGAAGCGTATTGCCAGGACATGCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAAt                                                                                                       >  2:853627/1‑81 (MQ=255)
                                                                      tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAAt                                                                                                       <  1:853627/81‑1 (MQ=255)
                                                                      tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa                                                        >  1:474132/1‑128 (MQ=255)
                                                                      tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa                                                        <  2:474132/128‑1 (MQ=255)
                                                                       gCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAat                                            <  1:1383517/139‑1 (MQ=255)
                                                                         aaCAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGaaaaa                                                                                                         <  1:549347/76‑1 (MQ=255)
                                                                         aaCAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGaaaaa                                                                                                         >  2:549347/1‑76 (MQ=255)
                                                                         aaCAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAg                                                                                                     <  1:196361/80‑1 (MQ=255)
                                                                         aaCAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAg                                                                                                     >  2:196361/1‑80 (MQ=255)
                                                                         aaCAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcct                                                                     <  1:859209/112‑1 (MQ=255)
                                                                         aaCAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACg                                                      >  1:700452/1‑127 (MQ=255)
                                                                         aaCAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACg                                                      <  2:700452/127‑1 (MQ=255)
                                                                         aaCAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAAACCGTGAGGAATACGGGTTAcct                                                                     >  2:859209/1‑112 (MQ=255)
                                                                           cAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGc                                        >  2:799764/1‑139 (MQ=255)
                                                                                gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATg                                         >  2:1137567/1‑133 (MQ=255)
                                                                                gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATg                                         <  1:1137567/133‑1 (MQ=255)
                                                                                gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGAtc                                   >  1:880137/1‑139 (MQ=255)
                                                                                   tATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATAc                                                                              <  2:35893/93‑1 (MQ=255)
                                                                                   tATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATAc                                                                              >  1:35893/1‑93 (MQ=255)
                                                                                           ggACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGa                                     <  2:436859/126‑1 (MQ=255)
                                                                                           ggACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGAAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGa                                     >  1:436859/1‑126 (MQ=255)
                                                                                            gACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCg                                      >  2:596407/1‑124 (MQ=255)
                                                                                            gACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCg                                      <  1:596407/124‑1 (MQ=255)
                                                                                                 ccccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAg                                                                                                     >  1:895195/1‑56 (MQ=255)
                                                                                                 ccccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAg                                                                                                     <  2:895195/56‑1 (MQ=255)
                                                                                                 ccccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATg                                         >  2:534361/1‑116 (MQ=255)
                                                                                                 ccccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATg                                         <  1:534361/116‑1 (MQ=255)
                                                                                                 ccccGCACCACTGAAACGCCGTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGATTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCaagc                  >  2:842080/1‑137 (MQ=255)
                                                                                                  cccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGtt                                                               <  2:967207/93‑1 (MQ=255)
                                                                                                  cccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGtt                                                               >  1:967207/1‑93 (MQ=255)
                                                                                                      caccacTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTgag                                                                                    >  2:983213/1‑68 (MQ=255)
                                                                                                      caccacTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTgag                                                                                    <  1:983213/68‑1 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt                                                                         >  2:28513/1‑74 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt                                                                         <  1:28513/74‑1 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcctc                                                                    <  2:182725/79‑1 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcctc                                                                    >  1:182725/1‑79 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACg                                                      >  1:1355944/1‑93 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACg                                                      <  2:1355944/93‑1 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGa                                     >  2:571097/1‑110 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGa                                     <  1:571097/110‑1 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCa                                <  1:1060056/115‑1 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCa                                >  2:1060056/1‑115 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTg        >  2:660369/1‑139 (MQ=255)
                                                                                                           cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTg        >  1:1417977/1‑139 (MQ=255)
                                                                                                               aaCGGCTTCTTCCCGACCAAAAACGGATTGAAAAAAAAATCGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGTAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGAtct    <  1:956777/139‑1 (MQ=255)
                                                                                                                 cGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCg  >  2:1131213/1‑139 (MQ=255)
                                                                                                                   ccTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGcc           >  1:284795/1‑128 (MQ=255)
                                                                                                                   ccTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGcc           <  2:284795/128‑1 (MQ=255)
                                                                                                                      tcttcCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa                                                        >  1:385049/1‑80 (MQ=255)
                                                                                                                      tcttcCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa                                                        <  2:385049/80‑1 (MQ=255)
                                                                                                                         tcCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGa                                     >  1:257042/1‑96 (MQ=255)
                                                                                                                         tcCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGa                                     <  2:257042/96‑1 (MQ=255)
                                                                                                                                |                                                                                                                           
TCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCG  >  NC_000913/2475123‑2475374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: