Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,525,930	C→T	*309* (TAG→TAA)	yfeR ←	transcriptional regulator of yefH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,525,930	0	C	T	100.0%	204.7 / NA	65	*309* (TAG→TAA)	yfeR	transcriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/31);  total (34/31)

ATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAC  >  NC_000913/2525808‑2526046                                                                                                                           |                                                                                                                     actacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACg                                                                                                      <  2:611140‑M2/21‑1 (MQ=255)     cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                  >  1:56461‑M2/115‑139 (MQ=255)          attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt                                                                                             >  1:915403‑M2/110‑139 (MQ=255)          attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt                                                                                             >  2:350902‑M2/110‑139 (MQ=255)               gtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                  <  1:651103‑M2/9‑1 (MQ=255)               gtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                  >  2:651103‑M2/105‑113 (MQ=255)                tatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                <  1:443501‑M2/11‑1 (MQ=255)                tatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                >  2:443501‑M2/104‑114 (MQ=255)                  tcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                     <  2:915403‑M2/38‑1 (MQ=255)                    gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaacgtaacttctgattgattactgccggtgcttccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGc                                                                                   >  1:1332360‑M2/100‑139 (MQ=255)                     atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGcc                                                                                  >  1:647829‑M2/99‑139 (MQ=255)                         gcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa                                                                              <  1:89569‑M2/45‑1 (MQ=255)                          cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtat                                                                                                                   <  2:1261191‑M2/8‑1 (MQ=255)                          cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtat                                                                                                                   >  1:1261191‑M2/94‑101 (MQ=255)                          cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg                                                                             >  2:890095‑M2/94‑139 (MQ=255)                              tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattgctgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                         <  1:890095‑M2/50‑1 (MQ=255)                               gacaaactggtcgatgatttttaccaggatgctataatctactccatcaaatttaacttctcattgattactgccgcggctcccgccaCACTTTATATCTGATACACCGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                        <  2:104810‑M2/51‑1 (MQ=255)                                    cctgggctgagatttctaccaggatgcgataatctactccaacaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCCGAAACAACGGAGCCCCTTCCCGCCCAGCCATTAGCGCATTcccc                                                                   <  2:1332360‑M2/56‑1 (MQ=255)                                    actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                   <  1:1085704‑M2/56‑1 (MQ=255)                                      tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                 >  1:104810‑M2/82‑139 (MQ=255)                                       gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTgg                                                                >  1:199218‑M2/81‑139 (MQ=255)                                         gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                >  1:575454‑M2/79‑89 (MQ=255)                                         gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc                                                                                                                <  2:575454‑M2/11‑1 (MQ=255)                                         gcgaagatttttaccaggatgcgataatctactccagcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtc                                                              >  2:431376‑M2/79‑139 (MQ=255)                                                 ttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                      <  1:350902‑M2/69‑1 (MQ=255)                                                         ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                      >  1:227168‑M2/63‑99 (MQ=255)                                                         ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                      <  2:227168‑M2/37‑1 (MQ=255)                                                          gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATa                                             >  2:1140493‑M2/62‑139 (MQ=255)                                                           atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                  <  1:849738‑M2/25‑1 (MQ=255)                                                           atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaa                                            >  2:1027636‑M2/61‑139 (MQ=255)                                                           atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaa                                            <  1:1027636‑M2/79‑1 (MQ=255)                                                           atgcgataatctactccatcaaatttaacttcagattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                  >  2:849738‑M2/61‑85 (MQ=255)                                                             gcgataatctactccatcgaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                     <  1:822847‑M2/38‑1 (MQ=255)                                                             gcgataatctactccatcgaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                     >  2:822847‑M2/59‑96 (MQ=255)                                                                           catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTTTATCTGATACCACGGGTCCCCTTCCCGCCCAGCCATTACCGCATTGCCCTGGTCCCGTCCCTCGTCCCCTTCAGCTTCaggggcggga                            >  2:1020594‑M2/45‑137 (MQ=255)                                                                           catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                            >  1:1242145‑M2/45‑139 (MQ=255)                                                                             tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                   >  2:522039‑M2/43‑98 (MQ=255)                                                                             tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                   <  1:522039‑M2/56‑1 (MQ=255)                                                                             tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                           >  2:1306077‑M2/43‑138 (MQ=255)                                                                             tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                           <  1:1306077‑M2/96‑1 (MQ=255)                                                                             tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCACCTTACCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATCAAGCTTCAGCGGCGGTGGa                          >  2:1000015‑M2/43‑139 (MQ=255)                                                                                  tttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtc                                                              >  1:389689‑M2/38‑98 (MQ=255)                                                                                  tttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtc                                                              <  2:389689‑M2/61‑1 (MQ=255)                                                                                  tttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                     <  2:1242145‑M2/102‑1 (MQ=255)                                                                                     aacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc                                                                                        <  2:404157‑M2/35‑1 (MQ=255)                                                                                     aacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc                                                                                        >  1:404157‑M2/35‑69 (MQ=255)                                                                                       cttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                >  2:482342‑M2/33‑139 (MQ=255)                                                                                          ctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                     >  1:1049275‑M2/30‑131 (MQ=255)                                                                                          ctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                     <  2:1049275‑M2/102‑1 (MQ=255)                                                                                           tgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                       <  1:1246945‑M2/36‑1 (MQ=255)                                                                                           tgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                       >  2:1246945‑M2/29‑64 (MQ=255)                                                                                           tgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttct            <  1:431376‑M2/111‑1 (MQ=255)                                                                                                    actgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                  <  1:244514‑M2/105‑1 (MQ=255)                                                                                                    actgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                  >  2:244514‑M2/20‑124 (MQ=255)                                                                                                     ctgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAc  <  2:647829‑M2/121‑1 (MQ=255)                                                                                                       gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc                                                                                            <  2:282433‑M2/31‑1 (MQ=255)                                                                                                       gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc                                                                                            >  1:1318012‑M2/17‑47 (MQ=255)                                                                                                       gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc                                                                                            <  2:1318012‑M2/31‑1 (MQ=255)                                                                                                       gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc                                                                                            >  1:282433‑M2/17‑47 (MQ=255)                                                                                                       gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGcc                                                                    <  1:28017‑M2/55‑1 (MQ=255)                                                                                                       gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGcc                                                                    >  2:28017‑M2/17‑71 (MQ=255)                                                                                                           gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                          >  2:727823‑M2/13‑109 (MQ=255)                                                                                                           gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                          <  1:727823‑M2/97‑1 (MQ=255)                                                                                                                cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                    >  1:1168142‑M2/8‑110 (MQ=255)                                                                                                                cccggcaCACTTTATATCGGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                    <  2:1168142‑M2/103‑1 (MQ=255)                                                                                                                           |                                                                                                                     ATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAC  >  NC_000913/2525808‑2526046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑