Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,618,075	C→T	*234* (TAG→TAA)	hda ←	ATPase regulatory factor involved in DnaA inactivation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,618,075	0	C	T	100.0%	204.6 / NA	63	*234* (TAG→TAA)	hda	ATPase regulatory factor involved in DnaA inactivation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/31);  total (32/31)

TCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAA  >  NC_000913/2617949‑2618204                                                                                                                               |                                                                                                                                  tCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAACCACCTTATTTACAACTTCAGa                                                                                                                       >  1:843339/1‑139 (MQ=255) tCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGa                                                                                                                       >  2:569094/1‑139 (MQ=255)                 aTTGTCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACg                                                                                                       >  2:1073513/1‑139 (MQ=255)                       ggATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                 <  2:509391/139‑1 (MQ=255)                       ggATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                 <  2:356632/139‑1 (MQ=255)                         aTGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTc                                                                                               <  1:150210/139‑1 (MQ=255)                           gCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAg                                                                                             >  1:781503/1‑139 (MQ=255)                                      cACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTgcg                                                                                  <  2:781503/139‑1 (MQ=255)                                             tATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAt                                                                           <  1:1073513/139‑1 (MQ=255)                                               tCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTa                                                                                                                                 >  2:1194180/1‑83 (MQ=255)                                                    ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTGAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                    >  2:940530/1‑139 (MQ=255)                                                    ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTGAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                    <  2:880056/139‑1 (MQ=255)                                                    ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                    >  1:1130628/1‑139 (MQ=255)                                                    ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                    >  1:454584/1‑139 (MQ=255)                                                    acTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTGGCGCGGTAATCGACGCa                                                                    >  1:880056/2‑139 (MQ=255)                                                      tACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAt                                                                           >  2:705195/1‑130 (MQ=255)                                                      tACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAt                                                                           <  1:705195/130‑1 (MQ=255)                                                        cGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTa                                                                                                                                 <  2:213667/74‑1 (MQ=255)                                                                 tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACTGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAAc                                                           <  1:1308226/135‑1 (MQ=255)                                                                 tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACTGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAAc                                                           >  2:1308226/1‑135 (MQ=255)                                                                 tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                            <  2:1213075/134‑1 (MQ=255)                                                                 tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                            >  1:1213075/1‑134 (MQ=255)                                                                 tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGAt                                                       >  2:1088596/1‑139 (MQ=255)                                                                    aTTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                    <  1:569094/139‑1 (MQ=255)                                                                    aTTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                    <  2:454584/139‑1 (MQ=255)                                                                        aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                    <  2:707904/135‑1 (MQ=255)                                                                        aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                    >  1:707904/1‑135 (MQ=255)                                                                         cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGcgcg                                                                                <  1:738113/106‑1 (MQ=255)                                                                         cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGcgcg                                                                                >  2:738113/1‑106 (MQ=255)                                                                           tCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCAt                                             >  1:376729/1‑139 (MQ=255)                                                                           tCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCAt                                             >  1:183567/1‑139 (MQ=255)                                                                              cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACTATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCAACg                                                                      <  2:921127/111‑1 (MQ=255)                                                                              cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                      >  1:921127/1‑111 (MQ=255)                                                                                        aTAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                 >  1:1203299/1‑74 (MQ=255)                                                                                        aTAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                 <  2:1203299/74‑1 (MQ=255)                                                                                        aTAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcgc                                >  2:654382/1‑139 (MQ=255)                                                                                            ggCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACgg                                                                                                      <  2:1371689/65‑1 (MQ=255)                                                                                            ggCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACgg                                                                                                      >  1:1371689/1‑65 (MQ=255)                                                                                            ggCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                    <  1:309674/115‑1 (MQ=255)                                                                                            ggCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                    >  2:309674/1‑115 (MQ=255)                                                                                             gCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGt                                                                              >  2:848402/1‑88 (MQ=255)                                                                                             gCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGt                                                                              <  1:848402/88‑1 (MQ=255)                                                                                             gCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                      <  1:671679/96‑1 (MQ=255)                                                                                             gCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                      >  2:671679/1‑96 (MQ=255)                                                                                             gCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTtc                           >  2:71767/1‑139 (MQ=255)                                                                                                  cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                      >  1:901806/1‑91 (MQ=255)                                                                                                  cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                      <  2:901806/91‑1 (MQ=255)                                                                                                  cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                  >  2:856877/1‑111 (MQ=255)                                                                                                  cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                  <  1:856877/111‑1 (MQ=255)                                                                                                  cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGt                      >  1:58486/1‑139 (MQ=255)                                                                                                      cggcATCCGTCAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAg                  <  2:843339/137‑1 (MQ=255)                                                                                                           tCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                    >  1:169926/1‑116 (MQ=255)                                                                                                           tCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                    <  2:169926/116‑1 (MQ=255)                                                                                                                 aaTAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                         >  1:1237396/1‑73 (MQ=255)                                                                                                                 aaTAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                         <  2:1237396/73‑1 (MQ=255)                                                                                                                   tAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAg     <  2:376729/139‑1 (MQ=255)                                                                                                                   tAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAg     <  2:183567/139‑1 (MQ=255)                                                                                                                     aaCACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaa   <  1:71767/139‑1 (MQ=255)                                                                                                                      acacCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa  <  1:940530/139‑1 (MQ=255)                                                                                                                          cTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTgcg                                                                                  >  2:72603/1‑55 (MQ=255)                                                                                                                          cTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTgcg                                                                                  <  1:72603/55‑1 (MQ=255)                                                                                                                           ttATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa  >  2:784206/1‑134 (MQ=255)                                                                                                                           ttATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa  <  1:784206/134‑1 (MQ=255)                                                                                                                               |                                                                                                                                  TCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAA  >  NC_000913/2617949‑2618204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑