Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,685,561	T→C	intergenic (‑54/‑274)	glyA ← / → hmp	serine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,685,561	0	T	C	100.0%	201.4 / NA	55	intergenic (‑54/‑274)	glyA/hmp	serine hydroxymethyltransferase/fused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/28);  total (27/28)

CCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCTGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTCT  >  NC_000913/2685429‑2685696                                                                                                                                     |                                                                                                                                        ccTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGa                                                                                                                                   <  1:1199411/139‑1 (MQ=255)         aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACg                                                                                                                              >  2:220939/1‑136 (MQ=255)         aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACg                                                                                                                              <  1:220939/136‑1 (MQ=255)         aCTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCg                                                                                                                           >  2:1349902/1‑139 (MQ=255)           tttttCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                         <  1:1172674/139‑1 (MQ=255)           tttttCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                         <  1:551024/139‑1 (MQ=255)               tCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGTCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGt                                                                                                                     >  2:921678/1‑139 (MQ=255)                           gCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACACTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCa                                                                                                         <  2:927928/139‑1 (MQ=255)                                ccACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgtg                                                                                                    <  1:921678/139‑1 (MQ=255)                                 cacaGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGa                                                                                                                          <  1:1068305/116‑1 (MQ=255)                                 cacaGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGa                                                                                                                          >  2:1068305/1‑116 (MQ=255)                                      ttCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              >  1:625451/1‑139 (MQ=255)                                         ggCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              <  1:1321637/136‑1 (MQ=255)                                         ggCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              >  2:871736/1‑136 (MQ=255)                                         ggCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              <  1:871736/136‑1 (MQ=255)                                         ggCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              >  2:1321637/1‑136 (MQ=255)                                              catAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGtat                                                                                                                   >  2:1383792/1‑110 (MQ=255)                                              catAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGtat                                                                                                                   <  1:1383792/110‑1 (MQ=255)                                              catAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAAc                                                                                             >  2:1298771/1‑132 (MQ=255)                                              catAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAAc                                                                                             <  1:1298771/132‑1 (MQ=255)                                                       cAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgt                                                                                                     >  1:640751/1‑115 (MQ=255)                                                       cAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgt                                                                                                     <  2:640751/115‑1 (MQ=255)                                                               aTTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGc                                                                     >  2:1101667/1‑139 (MQ=255)                                                                 ttCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              >  1:313922/1‑112 (MQ=255)                                                                 ttCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATaa                                                                                              <  2:313922/112‑1 (MQ=255)                                                                  tCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCaaa                                                                  <  2:1366405/139‑1 (MQ=255)                                                                      gCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtg                                                                                                      >  2:1274701/1‑99 (MQ=255)                                                                      gCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtg                                                                                                      <  1:1274701/99‑1 (MQ=255)                                                                      gCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              <  2:1265312/139‑1 (MQ=255)                                                                        tttAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGc                                                                     >  2:1324430/1‑130 (MQ=255)                                                                        tttAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGc                                                                     <  1:1324430/130‑1 (MQ=255)                                                                           aaCATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCt                                                         <  2:625451/139‑1 (MQ=255)                                                                                  gCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCt                                                  >  2:483744/1‑139 (MQ=255)                                                                                        ccTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGa                                                                                                                          <  2:376491/61‑1 (MQ=255)                                                                                        ccTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGa                                                                                                                          >  1:376491/1‑61 (MQ=255)                                                                                            aCTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCt                                        <  1:1069664/139‑1 (MQ=255)                                                                                              tCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCttt                                      <  1:483744/139‑1 (MQ=255)                                                                                                aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACg                                                                                                                              <  1:792831/49‑1 (MQ=255)                                                                                                aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACg                                                                                                                              >  2:792831/1‑49 (MQ=255)                                                                                                aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              <  1:1068433/113‑1 (MQ=255)                                                                                                aGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              >  2:1068433/1‑113 (MQ=255)                                                                                                  cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgtg                                                                                                    <  1:814420/73‑1 (MQ=255)                                                                                                  cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgtg                                                                                                    >  2:814420/1‑73 (MQ=255)                                                                                                  cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgt                                                                                                     <  2:751639/72‑1 (MQ=255)                                                                                                  cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATtgt                                                                                                     >  1:751639/1‑72 (MQ=255)                                                                                                  cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              >  2:1418736/1‑111 (MQ=255)                                                                                                  cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGAt                                                              <  1:1418736/111‑1 (MQ=255)                                                                                                  cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGgag                                  >  1:671952/1‑139 (MQ=255)                                                                                                  cTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGgag                                  >  2:919832/1‑139 (MQ=255)                                                                                                      cAATAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATa                              >  2:1109722/1‑139 (MQ=255)                                                                                                        aTAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                         >  2:323941/1‑46 (MQ=255)                                                                                                        aTAAAATTTTTGGCCTTTATAGGCGGTCCCGTTGGACAACGGCGaa                                                                                                                         <  1:323941/46‑1 (MQ=255)                                                                                                                                ggTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATaaa                            <  1:983542/115‑1 (MQ=255)                                                                                                                                ggTCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATaaa                            >  2:983542/1‑115 (MQ=255)                                                                                                                                  tCCCGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTCt  >  2:310956/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        CCTGACGTACTTTTTCCTGCTCCATAGCCTGCCACAGTTCGGCATCATAATCGGCAATGTTCATTTCACGCTTTAACATCCGCATCTCCTGACTCAGCTAACAATAAAATTTTTGGCCTTTATAGGCGGTCCTGTTGGACAACGGCGAACAGTATAACCGAATCATTGTGCGATAACAGGTCTTGACAAAGGAATTTACGCAAACGATTACCTTCAGGCTACGCAAGGCTTTGGAGAATAAAGAGCTTGCAACCGGAAACGGATTTCT  >  NC_000913/2685429‑2685696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑