Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,724,448	C→T	H107H (CAC→CAT)  *433* (TAG→TAA)	yfiM → kgtP ←	putative lipoprotein alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,448	0	C	T	100.0%	156.9 / NA	49	H107H (CAC→CAT)  *433* (TAG→TAA)	yfiM kgtP	putative lipoprotein alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/25);  total (24/25)

CTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACT  >  NC_000913/2724318‑2724578                                                                                                                                   |                                                                                                                                   ctGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACg                                                                                                                            >  2:1027687/1‑139 (MQ=255)  tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                           <  2:1267098/139‑1 (MQ=255)    tCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                           >  1:756085/1‑137 (MQ=255)    tCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                           <  2:756085/137‑1 (MQ=255)    tCAGTTCGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCAt                                                                                                                         >  1:596731/1‑139 (MQ=255)              ggCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCcccttccct                                                                                                               <  1:1027687/139‑1 (MQ=255)                 gTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                            <  2:325866/139‑1 (MQ=255)                 gTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                            >  1:325866/1‑139 (MQ=255)                                         ccccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGa                                                                                    >  2:769101/1‑139 (MQ=255)                                         ccccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGa                                                                                    >  2:407224/1‑139 (MQ=255)                                                 ggAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccac                                                                            <  2:596731/139‑1 (MQ=255)                                                      ggCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACgg                                                                       >  1:31450/1‑139 (MQ=255)                                                        ctggagctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGa                                                                                           >  1:1231975/1‑117 (MQ=255)                                                        ctggagctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGa                                                                                           <  2:1231975/117‑1 (MQ=255)                                                        ctggagctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGcc                                                                     >  1:844544/1‑139 (MQ=255)                                                        ctggagctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGcc                                                                     <  1:430189/139‑1 (MQ=255)                                                            agctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGaa                                                                                          >  1:60127/1‑114 (MQ=255)                                                            agctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGaa                                                                                          <  2:60127/114‑1 (MQ=255)                                                            agctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCa                                                                 >  2:1215085/1‑139 (MQ=255)                                                                ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                                             <  2:542125/139‑1 (MQ=255)                                                                            ccTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCagaa                                                 <  2:844544/139‑1 (MQ=255)                                                                                   tGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCt                                          <  1:1215085/139‑1 (MQ=255)                                                                                     tCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                            <  1:189634/71‑1 (MQ=255)                                                                                     tCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                            >  2:189634/1‑71 (MQ=255)                                                                                     tCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGt                                        >  2:260035/1‑139 (MQ=255)                                                                                             gCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTc                                <  2:31450/139‑1 (MQ=255)                                                                                               aaGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCt                              <  1:407224/139‑1 (MQ=255)                                                                                               aaGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCt                              <  1:769101/139‑1 (MQ=255)                                                                                                  cACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAtt                           <  1:260035/139‑1 (MQ=255)                                                                                                   aCCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTg                          <  2:897870/139‑1 (MQ=255)                                                                                                           taCCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGc                  >  1:99043/1‑139 (MQ=255)                                                                                                               gTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACa              >  1:1032103/1‑139 (MQ=255)                                                                                                               gTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACa              <  2:1032103/139‑1 (MQ=255)                                                                                                                 cTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATg                                                                                                       >  2:1072278/1‑48 (MQ=255)                                                                                                                 cTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATg                                                                                                       <  1:1072278/48‑1 (MQ=255)                                                                                                                     cAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTca                                                           >  2:1327217/1‑88 (MQ=255)                                                                                                                     cAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTca                                                           <  1:1327217/88‑1 (MQ=255)                                                                                                                     cAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTc                                     >  2:1414454/1‑110 (MQ=255)                                                                                                                     cAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTc                                     <  1:1414454/110‑1 (MQ=255)                                                                                                                        cTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGt     >  2:325732/1‑139 (MQ=255)                                                                                                                          gACCCGTCATTAAAGACGCATCCCCTTCCGTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACa              <  2:903402/128‑1 (MQ=255)                                                                                                                          gACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACa              >  1:903402/1‑128 (MQ=255)                                                                                                                           aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCa                                                                 >  1:615473/1‑76 (MQ=255)                                                                                                                           aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCa                                                                 <  2:615473/76‑1 (MQ=255)                                                                                                                           aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATAc                                                      >  1:1299768/1‑87 (MQ=255)                                                                                                                           aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATAc                                                      <  2:1299768/87‑1 (MQ=255)                                                                                                                           aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTa    >  2:1057592/1‑137 (MQ=255)                                                                                                                           aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTa    <  1:1057592/137‑1 (MQ=255)                                                                                                                           aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACt  >  1:813236/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                   CTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACT  >  NC_000913/2724318‑2724578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑