Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,737,495	G→A	*114* (TAG→TAA)	raiA →	cold shock protein associated with 30S ribosomal subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,737,495	0	G	A	100.0%	156.6 / NA	50	*114* (TAG→TAA)	raiA	cold shock protein associated with 30S ribosomal subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

TATGTACACCGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGTCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCT  >  NC_000913/2737360‑2737629                                                                                                                                        |                                                                                                                                       tatGTACACCGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATcc                                                                                                                                     >  1:359278/1‑139 (MQ=255) tatGTACACCGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATcc                                                                                                                                     <  1:788901/139‑1 (MQ=255)  atGTACACCGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCt                                                                                                                                    <  2:359278/139‑1 (MQ=255)          cGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGTAGAAGTTGAAGAAGAGTAATCCtt                                                                                                                                   <  2:968363/132‑1 (MQ=255)          cGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTg                                                                                                                            >  1:1105233/1‑139 (MQ=255)          cGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTg                                                                                                                            >  2:304322/1‑139 (MQ=255)          cGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTg                                                                                                                            >  2:111041/1‑139 (MQ=255)          cGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGAACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCtt                                                                                                                                   >  1:968363/1‑132 (MQ=255)            cAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAg                                                                                                                          >  1:1279176/1‑139 (MQ=255)            cAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAg                                                                                                                          >  1:1362159/1‑139 (MQ=255)            cAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAg                                                                                                                          >  1:157840/1‑139 (MQ=255)                  aCGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATc                                                                                                                    >  2:304391/1‑139 (MQ=255)                                  gCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCgg                                                                                                    >  2:46121/1‑139 (MQ=255)                                   cTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCggg                                                                                                   <  2:1105233/139‑1 (MQ=255)                                   cTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCggg                                                                                                   <  1:304391/139‑1 (MQ=255)                                        aCGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt                                                                                              <  1:46121/139‑1 (MQ=255)                                        aCGGCAGCTCAATAAACTGCAGCACAAACGCGAAGCACGTCGAGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt                                                                                              <  2:1182262/139‑1 (MQ=255)                                            cAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCt                                                                                                                                    >  2:1101034/1‑97 (MQ=255)                                            cAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCt                                                                                                                                    <  1:1101034/97‑1 (MQ=255)                                            cAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGttttt                                                                                          >  2:226013/1‑139 (MQ=255)                                                 cAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt                                                                                              >  1:591362/1‑130 (MQ=255)                                                 cAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt                                                                                              <  2:591362/130‑1 (MQ=255)                                                   aTAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGAc                                                                                   <  2:159225/139‑1 (MQ=255)                                                   aTAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGAc                                                                                   <  2:776469/139‑1 (MQ=255)                                                     aaaCTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAg                                                                                 <  2:1362159/139‑1 (MQ=255)                                                        cTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGt                                                                              <  1:226013/139‑1 (MQ=255)                                                              cacaAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAgt                                                                                                                         <  1:1048777/90‑1 (MQ=255)                                                              cacaAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAgt                                                                                                                         >  2:1048777/1‑90 (MQ=255)                                                                   aGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTAc                                                                   >  2:749884/1‑139 (MQ=255)                                                                      cGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACggg                                                                <  1:111041/139‑1 (MQ=255)                                                                        aaGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgta                                                              <  1:216278/139‑1 (MQ=255)                                                                        aaGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgta                                                              <  1:304322/139‑1 (MQ=255)                                                                        aaGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgta                                                              <  2:157840/139‑1 (MQ=255)                                                                               tcgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgtactgtact                                                       >  2:1230801/1‑139 (MQ=255)                                                                                  tGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt                                                                                              >  1:1314073/1‑97 (MQ=255)                                                                                  tGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt                                                                                              <  2:1314073/97‑1 (MQ=255)                                                                                   gCCGCAACATCGGTGAAAGACTCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTTTTGACAGCGTGaa                                                                           <  2:22656/115‑1 (MQ=255)                                                                                   gCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCGTTCGGGCGCGTTTTTTGTTGACAGCGTGaa                                                                           >  1:22656/1‑115 (MQ=255)                                                                                     cGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTc                                                 <  1:1230801/139‑1 (MQ=255)                                                                                        aaCATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACt                                              >  2:901009/1‑139 (MQ=255)                                                                                          cATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACggg                                                                >  1:422038/1‑119 (MQ=255)                                                                                          cATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACggg                                                                <  2:422038/119‑1 (MQ=255)                                                                                                    aGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAAc                                                                        <  2:1177754/101‑1 (MQ=255)                                                                                                    aGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAAc                                                                        >  1:1177754/1‑101 (MQ=255)                                                                                                    aGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGaaacaaa                                  >  1:1246445/1‑139 (MQ=255)                                                                                                       cGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgta                                                              <  1:535314/108‑1 (MQ=255)                                                                                                       cGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgta                                                              >  2:535314/1‑108 (MQ=255)                                                                                                               tcgtcgAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAAcaca                       >  1:570978/1‑139 (MQ=255)                                                                                                                cgactaagaagTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACAt                      >  2:830876/6‑139 (MQ=255)                                                                                                                 gtcgAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATa                     <  2:1246445/139‑1 (MQ=255)                                                                                                                                    agtaatCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAg                                                   >  1:103516/1‑90 (MQ=255)                                                                                                                                    agtaatCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAg                                                   <  2:103516/90‑1 (MQ=255)                                                                                                                                    agtaatCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCt  >  1:870315/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       TATGTACACCGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGTCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCT  >  NC_000913/2737360‑2737629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑