Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,800,475 | G→A | *110* (TAG→TAA) | ygaM → | putative membrane‑anchored DUF883 family ribosome‑binding protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,800,475 | 0 | G | A | 100.0% | 231.2 / NA | 72 | *110* (TAG→TAA) | ygaM | putative membrane‑anchored DUF883 family ribosome‑binding protein |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (33/39); total (33/39) |
GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCT > NC_000913/2800344‑2800607 | gTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑aaaa < 2:1231333/139‑1 (MQ=255) gTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCATAATGC‑aaaa < 1:185522/139‑1 (MQ=255) gTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCATAATGC‑aaaa > 2:185522/1‑139 (MQ=255) ccAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAAt < 1:1023458/139‑1 (MQ=255) ccAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAAt < 2:1050144/139‑1 (MQ=255) cAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATg < 2:830954/139‑1 (MQ=255) tGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑aaaaa > 2:598061/1‑114 (MQ=255) tGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑aaaaa < 1:598061/114‑1 (MQ=255) cgcgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTg < 1:250269/130‑1 (MQ=255) cgcgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTg > 2:250269/1‑130 (MQ=255) cgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGcc < 1:178061/139‑1 (MQ=255) tCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGtt > 2:1046477/1‑139 (MQ=255) gAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATAcg < 1:802210/103‑1 (MQ=255) gAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATAcg > 2:802210/1‑103 (MQ=255) tGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTgg > 2:1208296/1‑139 (MQ=255) gggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa < 2:201988/139‑1 (MQ=255) ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑a > 1:1334474/1‑64 (MQ=255) ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑a < 2:1334474/64‑1 (MQ=255) cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGAccc > 2:311976/1‑84 (MQ=255) cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGAccc < 1:311976/84‑1 (MQ=255) cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATAACAAAAATCCCCCTAc > 1:1129755/1‑139 (MQ=255) tGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtt < 1:614216/107‑1 (MQ=255) tGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtt > 2:614216/1‑107 (MQ=255) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGa > 2:151713/1‑59 (MQ=255) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGa < 1:151713/59‑1 (MQ=255) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAccc > 2:526289/1‑139 (MQ=255) gtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑aaaa > 1:252768/1‑52 (MQ=255) gtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑aaaa < 2:252768/52‑1 (MQ=255) gtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGt > 2:1373593/1‑139 (MQ=255) aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa < 1:526289/139‑1 (MQ=255) aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa < 1:1373593/139‑1 (MQ=255) aTCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATTAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc < 1:8792/139‑1 (MQ=255) tCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt > 1:974521/1‑78 (MQ=255) tCGGCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATcc > 2:1158191/1‑139 (MQ=255) tCGGCGCACTGTGAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt < 2:974521/78‑1 (MQ=255) ggCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc < 1:259862/131‑1 (MQ=255) ggCGCACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc > 2:259862/1‑131 (MQ=255) cgcACTGTTAAGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAggg > 1:1258929/1‑139 (MQ=255) aGCATGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc < 1:1158191/139‑1 (MQ=255) gCAAGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAcc < 2:1352619/139‑1 (MQ=255) tGCGCAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt < 2:456625/139‑1 (MQ=255) gcgcAAATCGTAATGCAAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc > 1:137909/1‑120 (MQ=255) gcgcAAATCGTAATGCAAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTACTTTTCGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc < 2:137909/120‑1 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCttt > 1:187626/1‑42 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCttt < 2:187626/42‑1 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAAt < 2:552383/85‑1 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAAt > 1:552383/1‑85 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa > 1:1162239/1‑91 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa < 1:644314/91‑1 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa > 2:644314/1‑91 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa < 1:1131837/109‑1 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa > 2:1131837/1‑109 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc > 2:1365726/1‑114 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc > 2:1421354/1‑114 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc < 1:1421354/114‑1 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc < 1:1365726/114‑1 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa > 2:1160006/1‑139 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa > 1:544444/1‑139 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTGTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa > 2:464153/1‑139 (MQ=255) gcgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGCCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa < 2:1162239/91‑1 (MQ=255) cgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt < 1:1046477/139‑1 (MQ=255) cgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt < 2:1258929/139‑1 (MQ=255) cgcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt < 1:1208296/139‑1 (MQ=255) gcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt > 2:1115348/1‑57 (MQ=255) gcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt < 1:1115348/57‑1 (MQ=255) gcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGt > 2:449237/1‑64 (MQ=255) gcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGt < 1:449237/64‑1 (MQ=255) gcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAtt > 2:557168/1‑139 (MQ=255) gcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt < 2:1256032/138‑1 (MQ=255) gcAAATCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt > 1:1256032/1‑138 (MQ=255) aaTCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc < 2:1129755/139‑1 (MQ=255) aTCGTAATGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCt < 1:1160006/139‑1 (MQ=255) | GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGC‑AAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCT > NC_000913/2800344‑2800607 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |