Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,271	G→A	A78V (GCA→GTA)	yqaB ←	fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,271	0	G	A	100.0%	146.2 / NA	46	A78V (GCA→GTA)	yqaB	fructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/21);  total (25/21)

CCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGT  >  NC_000913/2817143‑2817406                                                                                                                                 |                                                                                                                                        ccAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGtttt                                                                                                                               >  1:1320299/1‑139 (MQ=255)    gCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTc                                                                                                                            <  2:731384/139‑1 (MQ=255)       aTGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCAcg                                                                                                                         >  2:104277/1‑139 (MQ=255)                   tGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGCGGAAGCGGTTCGACGCTATCCATCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATg                                                                                                             <  1:632978/139‑1 (MQ=255)                   tGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCAcgcg                                                                                                                       <  2:1329186/129‑1 (MQ=255)                   tGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCAcgcg                                                                                                                       >  1:1329186/1‑129 (MQ=255)                   tGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATg                                                                                                             <  2:1320299/139‑1 (MQ=255)                             aCTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCgaga                                                                                                   <  1:882887/139‑1 (MQ=255)                              cTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAt                                                                                                  <  1:73404/139‑1 (MQ=255)                                   cGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc                                                                                              >  1:57901/1‑138 (MQ=255)                                   cGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc                                                                                              <  2:57901/138‑1 (MQ=255)                                             gCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCt                                                                                   <  1:1174841/139‑1 (MQ=255)                                                       gacgacCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGc                                                                         <  2:1117882/139‑1 (MQ=255)                                                               tGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCaa                                                                 >  1:507331/1‑139 (MQ=255)                                                                                aTCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGAt                                                >  2:1132369/1‑139 (MQ=255)                                                                                         agGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAgc                                       >  1:257980/1‑139 (MQ=255)                                                                                          ggAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCa                                                                                                                           <  1:546753/54‑1 (MQ=255)                                                                                          ggAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCa                                                                                                                           >  2:546753/1‑54 (MQ=255)                                                                                          ggAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAgcg                                      >  1:411311/1‑139 (MQ=255)                                                                                           gAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAg                                                                    >  2:555874/1‑108 (MQ=255)                                                                                           gAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAg                                                                    <  1:555874/108‑1 (MQ=255)                                                                                           gAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAgcgc                                     >  2:731897/1‑139 (MQ=255)                                                                                             aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCATGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCaa                                   >  2:889560/1‑139 (MQ=255)                                                                                             aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGAtt                                                                                        >  1:739651/1‑86 (MQ=255)                                                                                             aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGAtt                                                                                        <  2:739651/86‑1 (MQ=255)                                                                                             aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCaa                                   >  1:604193/1‑139 (MQ=255)                                                                                             aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCaa                                   >  2:1092727/1‑139 (MQ=255)                                                                                             aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCaa                                   >  1:1375383/1‑139 (MQ=255)                                                                                                ggTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAt                                                                                                  >  1:591680/1‑73 (MQ=255)                                                                                                ggTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAt                                                                                                  <  2:591680/73‑1 (MQ=255)                                                                                                 gTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCATGCGCTAACGCATGCGGGTCGAGTTCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAatcat                               <  1:889560/139‑1 (MQ=255)                                                                                                 gTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAatcat                               <  2:507331/139‑1 (MQ=255)                                                                                                           aTCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAata                     <  1:1092727/139‑1 (MQ=255)                                                                                                            tCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAatat                    >  2:741045/1‑139 (MQ=255)                                                                                                                 cagcaTACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTAc               >  2:830335/1‑139 (MQ=255)                                                                                                                  agcaTACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATg                                                                                                             <  2:245806/44‑1 (MQ=255)                                                                                                                  agcaTACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATg                                                                                                             >  1:245806/1‑44 (MQ=255)                                                                                                                      tACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGcc                                                                                             <  1:684789/56‑1 (MQ=255)                                                                                                                      tACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGcc                                                                                             >  2:684789/1‑56 (MQ=255)                                                                                                                      tACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGTGCGATCCATTAAGCGCAATCATCGCCTGAATATc                   <  2:943069/130‑1 (MQ=255)                                                                                                                      tACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATc                   >  1:943069/1‑130 (MQ=255)                                                                                                                         ttcttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTgg                                                     <  2:618068/93‑1 (MQ=255)                                                                                                                         ttcttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTgg                                                     >  1:618068/1‑93 (MQ=255)                                                                                                                         ttcttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGAcc       >  1:1034075/1‑139 (MQ=255)                                                                                                                           cttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCg                                                  <  2:111364/94‑1 (MQ=255)                                                                                                                           cttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCg                                                  >  1:111364/1‑94 (MQ=255)                                                                                                                             tactacTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAg   <  1:741045/139‑1 (MQ=255)                                                                                                                              actactTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGt  >  2:669889/1‑139 (MQ=255)                                                                                                                              actactTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGt  >  2:246954/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                        CCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGT  >  NC_000913/2817143‑2817406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑