Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,936,931	T→C	F450L (TTC→CTC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,936,931	0	T	C	100.0%	192.8 / NA	53	F450L (TTC→CTC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/27);  total (26/27)

CGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATG  >  NC_000913/2936796‑2937048                                                                                                                                        |                                                                                                                      cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTcc                                                                                                                    >  2:474826/1‑139 (MQ=255)  gcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGTTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGcctcct                                                                                                                   >  2:1289773/1‑139 (MQ=255)       aGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCg                                                                                                              <  2:376677/139‑1 (MQ=255)             ggTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAggcg                                                                                                        <  1:45104/139‑1 (MQ=255)                  cccGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTc                                                                                                   <  1:180043/139‑1 (MQ=255)                                   acTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCg                                                                                  >  1:1074300/1‑139 (MQ=255)                                    cTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCgt                                                                                 >  2:1175776/1‑139 (MQ=255)                                                 cAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACAtcatca                                                                    >  2:674090/1‑139 (MQ=255)                                                  aGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                   <  2:1068239/139‑1 (MQ=255)                                                          gCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTgg                                                           >  1:895590/1‑139 (MQ=255)                                                          gCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTgg                                                           >  2:514281/1‑139 (MQ=255)                                                             gACGCTTGCCTCGCCGTTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                        <  1:1289773/139‑1 (MQ=255)                                                             gACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                        <  1:323439/139‑1 (MQ=255)                                                              aCGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                   <  1:1165682/127‑1 (MQ=255)                                                              aCGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                   >  2:1165682/1‑127 (MQ=255)                                                                    gCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACAtcatca                                                                    >  1:743768/1‑120 (MQ=255)                                                                    gCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACAtcatca                                                                    <  2:743768/120‑1 (MQ=255)                                                                              tACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATcgcg                                       >  2:1281883/1‑139 (MQ=255)                                                                              tACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATcgcg                                       <  1:1334652/139‑1 (MQ=255)                                                                                      ggTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTg                               <  1:445103/139‑1 (MQ=255)                                                                                        tGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTg                               >  2:55170/1‑137 (MQ=255)                                                                                        tGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTg                               <  1:55170/137‑1 (MQ=255)                                                                                          cccGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAggcg                                                                                                        >  2:428982/1‑62 (MQ=255)                                                                                          cccGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAggcg                                                                                                        <  1:428982/62‑1 (MQ=255)                                                                                             ggCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTg                        >  1:14542/1‑139 (MQ=255)                                                                                                gATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                     <  1:1281883/139‑1 (MQ=255)                                                                                                     aCGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGcc                <  1:1175776/139‑1 (MQ=255)                                                                                                         aTACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGtt                                                                                              >  1:1202109/1‑57 (MQ=255)                                                                                                         aTACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGtt                                                                                              <  2:1202109/57‑1 (MQ=255)                                                                                                          tACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAgg           <  1:514281/139‑1 (MQ=255)                                                                                                            cTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAt         >  2:60844/1‑139 (MQ=255)                                                                                                             ttCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGtt                                                                                              <  1:792522/53‑1 (MQ=255)                                                                                                             ttCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGtt                                                                                              >  2:792522/1‑53 (MQ=255)                                                                                                             ttCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaaa                                                                  >  2:167922/1‑81 (MQ=255)                                                                                                             ttCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaaa                                                                  <  1:167922/81‑1 (MQ=255)                                                                                                              tCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAgg           >  1:67110/1‑135 (MQ=255)                                                                                                              tCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAgg           <  2:67110/135‑1 (MQ=255)                                                                                                              tCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAtgt       >  2:231487/1‑139 (MQ=255)                                                                                                                 gTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                        <  1:782355/87‑1 (MQ=255)                                                                                                                 gTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                        >  2:782355/1‑87 (MQ=255)                                                                                                                  tGGCGGCGGTTACTCTTTCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGAc                                                                                     >  1:895338/1‑57 (MQ=255)                                                                                                                  tGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGAc                                                                                     <  2:895338/57‑1 (MQ=255)                                                                                                                  tGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                        <  2:595986/86‑1 (MQ=255)                                                                                                                  tGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                        >  1:595986/1‑86 (MQ=255)                                                                                                                  tGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCAt   >  2:1211358/1‑139 (MQ=255)                                                                                                                   ggcggcGGTTACTCTTCCCGCCTCCTTACCGAAGgcggcg                                                                                                     >  2:1158669/1‑40 (MQ=255)                                                                                                                   ggcggcGGTTACTCTTCCCGCCTCCTTACCGAAGgcggcg                                                                                                     <  1:1158669/40‑1 (MQ=255)                                                                                                                   ggcggcGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATg  <  2:14542/139‑1 (MQ=255)                                                                                                                   ggcggcGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATg  <  1:231487/139‑1 (MQ=255)                                                                                                                   ggcggcGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATg  >  1:1262245/1‑139 (MQ=255)                                                                                                                    gcggcgGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTc                                                                              >  2:125063/1‑62 (MQ=255)                                                                                                                    gcggcgGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTc                                                                              <  1:125063/62‑1 (MQ=255)                                                                                                                      ggcggTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCg                                                                                                <  2:1248240/42‑1 (MQ=255)                                                                                                                      ggcggTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCg                                                                                                >  1:1248240/1‑42 (MQ=255)                                                                                                                                        |                                                                                                                      CGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATG  >  NC_000913/2936796‑2937048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑