Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,937,802 A→G D112G (GAC→GGC)  fucK → L‑fuculokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,937,8020AG97.4% 260.6 / ‑3.0 78D112G (GAC→GGC) fucKL‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (36/38);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

AACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTG  >  NC_000913/2937670‑2937937
                                                                                                                                    |                                                                                                                                       
aaCTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAatat                                                                                                                                   >  2:310620/1‑139 (MQ=255)
aaCTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAatat                                                                                                                                   >  1:1305663/1‑139 (MQ=255)
aaCTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTAGCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAatat                                                                                                                                   >  1:1405808/1‑139 (MQ=255)
    gactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGaa                                                                                                                               <  2:1044054/139‑1 (MQ=255)
     actgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAAc                                                                                                                              <  1:310620/139‑1 (MQ=255)
     actgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAAc                                                                                                                              >  2:603822/1‑139 (MQ=255)
               acaTCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAAtctc                                                                                                                    >  1:702353/1‑139 (MQ=255)
                 aTCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCg                                                                                                                  <  1:192927/139‑1 (MQ=255)
                      cgGTATCGCCGTCACCACCTTAGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGTACAg                                                                                                             <  2:832903/139‑1 (MQ=255)
                                                  gATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAg                                                                                 >  2:359885/1‑139 (MQ=255)
                                                   aTGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGc                                                                                >  2:1284973/1‑139 (MQ=255)
                                                      gcgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCtt                                                                             <  2:702353/139‑1 (MQ=255)
                                                       cgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTa                                                                           >  2:204449/1‑139 (MQ=255)
                                                         ctctGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGt                                                                         <  1:204449/139‑1 (MQ=255)
                                                          tctGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGt                                                                         <  1:1284973/139‑1 (MQ=255)
                                                          tctGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGt                                                                         >  1:768489/1‑139 (MQ=255)
                                                              gTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                     <  1:1358230/139‑1 (MQ=255)
                                                                 gATAAGCAAGGCAATCTGCTCTATCCGATGATTAGCTTGAAATGTCCGCGAACAGCAGCGGTTAGGGGTAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                  <  2:1405808/139‑1 (MQ=255)
                                                                  aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATAc                                                                 <  2:1084519/139‑1 (MQ=255)
                                                                   tAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                <  1:1008688/139‑1 (MQ=255)
                                                                    aaGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCa                                                                                                     <  1:413016/101‑1 (MQ=255)
                                                                    aaGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCa                                                                                                     >  2:413016/1‑101 (MQ=255)
                                                                    aaGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGt                                                               >  2:686176/1‑139 (MQ=255)
                                                                      gCAAGGCAATCTGCTCTATCCGATTATTGGCTGGAAATGTCCGCGAACAGCAGCGGTAATGCGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                     >  2:620294/1‑131 (MQ=255)
                                                                      gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCgg                                                                                   <  2:303593/117‑1 (MQ=255)
                                                                      gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCgg                                                                                   >  1:303593/1‑117 (MQ=255)
                                                                      gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                     <  1:620294/131‑1 (MQ=255)
                                                                      gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                  <  1:487570/134‑1 (MQ=255)
                                                                      gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                  >  2:487570/1‑134 (MQ=255)
                                                                      gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTta                                                             >  1:1063450/1‑139 (MQ=255)
                                                                       cAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtat                                                            <  1:359885/139‑1 (MQ=255)
                                                                        aaGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCt                                                                                           <  2:996179/107‑1 (MQ=255)
                                                                        aaGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCt                                                                                           >  1:996179/1‑107 (MQ=255)
                                                                        aaGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                  >  1:967951/1‑132 (MQ=255)
                                                                        aaGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                  <  2:967951/132‑1 (MQ=255)
                                                                         aGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                     <  2:1042523/128‑1 (MQ=255)
                                                                         aGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                     >  1:1042523/1‑128 (MQ=255)
                                                                          ggCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtatata                                                         <  1:603822/139‑1 (MQ=255)
                                                                           gCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATaa                                                        >  1:1032178/1‑139 (MQ=255)
                                                                               tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTCTGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                <  2:905267/127‑1 (MQ=255)
                                                                               tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCa                                                                                                                                       >  1:1422741/1‑56 (MQ=255)
                                                                               tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCa                                                                                                                                       <  2:1422741/56‑1 (MQ=255)
                                                                               tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                  >  2:574109/1‑125 (MQ=255)
                                                                               tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                  <  1:574109/125‑1 (MQ=255)
                                                                               tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                >  1:905267/1‑127 (MQ=255)
                                                                                    tctATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTg                                                                                                                                 >  1:1047585/1‑57 (MQ=255)
                                                                                    tctATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTg                                                                                                                                 <  2:1047585/57‑1 (MQ=255)
                                                                                      tATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGt                                             <  2:1032178/139‑1 (MQ=255)
                                                                                        tCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTg                                           >  2:1313230/1‑139 (MQ=255)
                                                                                        tCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTg                                           >  1:491075/1‑139 (MQ=255)
                                                                                           gATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTg                                                                                                       >  1:359810/1‑76 (MQ=255)
                                                                                           gATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTg                                                                                                       <  2:359810/76‑1 (MQ=255)
                                                                                             ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTg                                                                                                       >  2:1413768/1‑74 (MQ=255)
                                                                                             ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTg                                                                                                       <  1:1413768/74‑1 (MQ=255)
                                                                                             ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                     <  2:1085292/108‑1 (MQ=255)
                                                                                             ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                     >  1:1085292/1‑108 (MQ=255)
                                                                                                ttaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGaaaa                                   <  2:1063450/139‑1 (MQ=255)
                                                                                                  aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAg                                                                                                    >  2:1004948/1‑72 (MQ=255)
                                                                                                  aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAg                                                                                                    <  1:1004948/72‑1 (MQ=255)
                                                                                                  aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTg                                                                                          <  2:111628/82‑1 (MQ=255)
                                                                                                  aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTg                                                                                          >  1:111628/1‑82 (MQ=255)
                                                                                                  aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAg                                                                          <  1:177630/98‑1 (MQ=255)
                                                                                                  aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAg                                                                          >  2:177630/1‑98 (MQ=255)
                                                                                                      ggAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAg                                                                                                    >  1:608039/1‑68 (MQ=255)
                                                                                                      ggAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAg                                                                                                    <  2:608039/68‑1 (MQ=255)
                                                                                                       gAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGa                                                                                  <  2:642269/85‑1 (MQ=255)
                                                                                                       gAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGa                                                                                  >  1:642269/1‑85 (MQ=255)
                                                                                                       gAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                  >  2:326228/1‑101 (MQ=255)
                                                                                                       gAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                  <  1:326228/101‑1 (MQ=255)
                                                                                                        aaaTGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                           <  1:686176/139‑1 (MQ=255)
                                                                                                             tCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActg                      >  2:569033/1‑139 (MQ=255)
                                                                                                               cgcgAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCgg                                                                                   >  2:372595/1‑76 (MQ=255)
                                                                                                               cgcgAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCgg                                                                                   <  1:372595/76‑1 (MQ=255)
                                                                                                                             gTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                           <  1:1078891/118‑1 (MQ=255)
                                                                                                                             gTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                           >  2:1078891/1‑118 (MQ=255)
                                                                                                                                aTGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                <  2:327087/78‑1 (MQ=255)
                                                                                                                                aTGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                >  1:327087/1‑78 (MQ=255)
                                                                                                                                 tGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTg  <  2:768489/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
AACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTG  >  NC_000913/2937670‑2937937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: