Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,129,036	G→A	*255* (TAG→TAA)	glcC →	glycolate‑inducible glc operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,129,036	0	G	A	100.0%	172.0 / NA	53	*255* (TAG→TAA)	glcC	glycolate‑inducible glc operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/30);  total (23/30)

CAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTT  >  NC_000913/3128902‑3129162                                                                                                                                       |                                                                                                                               cAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGGTAACTgg                                                                                                                            >  2:977683/1‑139 (MQ=255)        tGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTaaa                                                                                                                     >  1:746972/1‑139 (MQ=255)         gCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAAt                                                                                                                    <  2:1429807/139‑1 (MQ=255)           cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                  <  2:694827/139‑1 (MQ=255)              aCGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCt                                                                                                               <  2:1079100/139‑1 (MQ=255)              aCGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCt                                                                                                               <  2:746972/139‑1 (MQ=255)                  cGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTc                                                                                                           <  2:1090901/139‑1 (MQ=255)                       aGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACa                                                                                                      <  1:438362/139‑1 (MQ=255)                             cagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                <  1:983109/139‑1 (MQ=255)                                       tCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTa                                                                                      <  2:1244999/139‑1 (MQ=255)                                       tCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTa                                                                                      <  1:729027/139‑1 (MQ=255)                                              cGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTTCCGCTGGAGACGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTcc                                                                               >  2:1023858/1‑139 (MQ=255)                                                 aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                            >  2:872157/1‑139 (MQ=255)                                                 aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                            >  1:1408666/1‑139 (MQ=255)                                                 aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                            >  2:699724/1‑139 (MQ=255)                                                 aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                                            >  1:1280659/1‑139 (MQ=255)                                                   cGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcccc                                                                          >  1:1096178/1‑139 (MQ=255)                                                     tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAAt                                                                                                                    <  2:1119517/95‑1 (MQ=255)                                                     tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAAt                                                                                                                    >  1:1119517/1‑95 (MQ=255)                                                      gAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAAt                                                                       <  2:1280659/139‑1 (MQ=255)                                                         aaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCg                                                                    <  1:742786/139‑1 (MQ=255)                                                              aTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               <  1:699724/139‑1 (MQ=255)                                                              aTCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               <  1:941022/139‑1 (MQ=255)                                                                        tatCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                                     <  2:1408666/139‑1 (MQ=255)                                                                             aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               <  1:1190807/124‑1 (MQ=255)                                                                             aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               >  2:1190807/1‑124 (MQ=255)                                                                             aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                                     >  1:482469/1‑134 (MQ=255)                                                                             aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                                     <  2:482469/134‑1 (MQ=255)                                                                             aGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGc                                                >  1:894292/1‑139 (MQ=255)                                                                                  gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                              <  2:1285538/56‑1 (MQ=255)                                                                                  gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                              >  2:318694/1‑56 (MQ=255)                                                                                  gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                              <  1:318694/56‑1 (MQ=255)                                                                                  gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACt                                                                                                                              >  1:1285538/1‑56 (MQ=255)                                                                                  gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGccc                                                                           >  1:422472/1‑107 (MQ=255)                                                                                  gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGccc                                                                           <  2:422472/107‑1 (MQ=255)                                                                                        caccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                                     >  1:1063990/1‑123 (MQ=255)                                                                                        caccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                                     <  2:1063990/123‑1 (MQ=255)                                                                                        caccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGtt                                     >  1:616298/1‑139 (MQ=255)                                                                                          ccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               >  2:1310753/1‑111 (MQ=255)                                                                                          ccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               <  1:1310753/111‑1 (MQ=255)                                                                                            aTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa                                 <  1:420694/139‑1 (MQ=255)                                                                                            aTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa                                 <  2:1096178/139‑1 (MQ=255)                                                                                            aTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa                                 <  2:593220/139‑1 (MQ=255)                                                                                             tttGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                  >  2:1210954/1‑57 (MQ=255)                                                                                             tttGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                  <  1:1210954/57‑1 (MQ=255)                                                                                                    cGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt                         <  2:894292/139‑1 (MQ=255)                                                                                                                 cGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAgcg            <  2:1295685/139‑1 (MQ=255)                                                                                                                   cTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               <  2:138857/86‑1 (MQ=255)                                                                                                                   cTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               >  1:138857/1‑86 (MQ=255)                                                                                                                         aTGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATc    >  2:1372402/1‑139 (MQ=255)                                                                                                                           gAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCtt  >  2:965067/1‑139 (MQ=255)                                                                                                                             aCCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               <  2:1288090/76‑1 (MQ=255)                                                                                                                             aCCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                                               >  1:1288090/1‑76 (MQ=255)                                                                                                                                       |                                                                                                                               CAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTT  >  NC_000913/3128902‑3129162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑