Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,134,823 G→A *231* (TAG→TAA yghT → putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,134,8230GA100.0% 86.0 / NA 28*231* (TAG→TAAyghTputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

GTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGC  >  NC_000913/3134717‑3134946
                                                                                                          |                                                                                                                           
gtggtgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                            >  1:1383903/1‑139 (MQ=255)
     gCACAACTGGTTGATGTCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                       >  1:410022/1‑139 (MQ=255)
                tGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGt                                                                            <  2:316180/139‑1 (MQ=255)
                   tgtCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                         <  2:410022/137‑1 (MQ=255)
                        aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  1:1278523/101‑1 (MQ=255)
                        aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          >  2:1278523/1‑101 (MQ=255)
                            cAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGa                                                                                                         <  1:1041722/98‑1 (MQ=255)
                            cAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGa                                                                                                         >  2:1041722/1‑98 (MQ=255)
                                                   tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  1:1289012/74‑1 (MQ=255)
                                                   tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          >  2:1289012/1‑74 (MQ=255)
                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          >  2:399354/1‑68 (MQ=255)
                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  2:359121/68‑1 (MQ=255)
                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          >  1:359121/1‑68 (MQ=255)
                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  1:399354/68‑1 (MQ=255)
                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                   >  2:282245/1‑139 (MQ=255)
                                                               aaaaaGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGa                                                                                                >  1:1150521/1‑72 (MQ=255)
                                                               aaaaaGCGATTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGa                                                                                                <  2:1150521/72‑1 (MQ=255)
                                                                       aTTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                         <  2:163395/87‑1 (MQ=255)
                                                                       aTTACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                         >  1:163395/1‑87 (MQ=255)
                                                                         tACGGATTTTATGACCGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt                   <  1:282245/139‑1 (MQ=255)
                                                                                       ccGCGCGGGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa     <  2:497831/139‑1 (MQ=255)
                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                               >  2:1270396/5‑46 (MQ=255)
                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                               <  1:1270396/42‑1 (MQ=255)
                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                            >  2:1369307/5‑49 (MQ=255)
                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                            <  1:1369307/45‑1 (MQ=255)
                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                               <  1:462166/74‑1 (MQ=255)
                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                               >  2:462166/5‑78 (MQ=255)
                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc  >  2:1185299/5‑139 (MQ=255)
                                                                                                          |                                                                                                                           
GTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGC  >  NC_000913/3134717‑3134946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: