Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,151,425	G→A	A187V (GCA→GTA)	exbB ←	membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,151,425	0	G	A	100.0%	256.9 / NA	79	A187V (GCA→GTA)	exbB	membrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/38);  total (41/38)

GCGGCGCTGGCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGA  >  NC_000913/3151293‑3151560                                                                                                                                     |                                                                                                                                        gcggcgCTGGCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGa                                                                                                                                   >  2:751798/1‑139 (MQ=255) gcggcgCTGGCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGa                                                                                                                                   >  1:1129778/1‑139 (MQ=255)       cTGGCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGa                                                                                                                             <  2:1425917/139‑1 (MQ=255)          gCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTg                                                                                                                          <  2:968079/139‑1 (MQ=255)          gCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTg                                                                                                                          <  1:137840/139‑1 (MQ=255)                    ccAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTg                                                                                                                          >  2:497384/1‑129 (MQ=255)                    ccAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTg                                                                                                                          <  1:497384/129‑1 (MQ=255)                             ggCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTc                                                                                                       >  2:1189678/1‑139 (MQ=255)                             ggCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTc                                                                                                       >  1:341506/1‑139 (MQ=255)                               cTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTg                                                                                                     >  1:541655/1‑139 (MQ=255)                                             tACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGC‑TCTGCGATACCCGGCGCaa                                                                                      <  1:1173537/139‑1 (MQ=255)                                              aCCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCaa                                                                                      >  2:525550/1‑139 (MQ=255)                                               ccTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAAc                                                                                     <  1:1189678/139‑1 (MQ=255)                                                  gcgcTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGAc                                                                                  <  2:541655/139‑1 (MQ=255)                                                  gcgcTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGAc                                                                                  >  1:83541/1‑139 (MQ=255)                                                    gcTGCAACATCACCCAGCATCGCTTTAAAGCTGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACTAGGCCTATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGgcg                                                                                         <  1:957646/130‑1 (MQ=255)                                                    gcTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGgcg                                                                                         >  2:957646/1‑130 (MQ=255)                                                    gcTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTg                                                                                >  1:1006230/1‑139 (MQ=255)                                                    gcTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCACTACCACGAGGCCGATTGCCGTTGCTAACAGAGc                                                                                                          >  1:723268/1‑113 (MQ=255)                                                    gcTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCACTACCACGAGGCCGATTGCCGTTGCTAACAGAGc                                                                                                          <  2:723268/113‑1 (MQ=255)                                                        cAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAg                                                                            >  1:1129422/1‑139 (MQ=255)                                                        cAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAg                                                                            >  2:906038/1‑139 (MQ=255)                                                             tCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAg                                                                       >  1:1027127/1‑139 (MQ=255)                                                             tCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTGATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAg                                                                       <  2:83541/139‑1 (MQ=255)                                                                cccAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTgg                                                                    >  1:536261/1‑139 (MQ=255)                                                                        cGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGtt                                                            >  2:101804/1‑139 (MQ=255)                                                                          cTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACg                                                                                                                                    <  2:1300979/65‑1 (MQ=255)                                                                          cTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACg                                                                                                                                    >  1:1300979/1‑65 (MQ=255)                                                                           tttAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTgc                                                         >  2:303232/1‑139 (MQ=255)                                                                            ttAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTgcg                                                        <  2:1006230/139‑1 (MQ=255)                                                                                     ccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGAt                                                    <  2:774301/134‑1 (MQ=255)                                                                                     ccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGAt                                                    >  1:774301/1‑134 (MQ=255)                                                                                         tCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATAc                                                                                               >  2:721777/1‑87 (MQ=255)                                                                                         tCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATAc                                                                                               <  1:721777/87‑1 (MQ=255)                                                                                          cTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAg                                          <  2:1027127/139‑1 (MQ=255)                                                                                                tGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTgcg                                                        <  2:1342742/119‑1 (MQ=255)                                                                                                tGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTgcg                                                        >  1:1342742/1‑119 (MQ=255)                                                                                                 gCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCa                                   <  1:101804/139‑1 (MQ=255)                                                                                                   aaaTACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatg                                 <  2:250150/139‑1 (MQ=255)                                                                                                   aaaTACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatg                                 <  1:906038/139‑1 (MQ=255)                                                                                                   aaaTACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatg                                 <  1:525550/139‑1 (MQ=255)                                                                                                   aaaTACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatg                                 <  2:1129422/139‑1 (MQ=255)                                                                                                       aCGTTATAGATAACGACCGCAGGAATCGCTAACACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGc                             >  1:331622/1‑139 (MQ=255)                                                                                                           tataGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCa                         >  2:139203/1‑139 (MQ=255)                                                                                                           tataGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCa                         >  2:1376514/1‑139 (MQ=255)                                                                                                             taGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGa                       <  1:139203/139‑1 (MQ=255)                                                                                                             taGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGa                       <  2:341506/139‑1 (MQ=255)                                                                                                                 tAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGc                                                                               >  1:1216885/1‑79 (MQ=255)                                                                                                                 tAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGc                                                                               <  2:1216885/79‑1 (MQ=255)                                                                                                                 tAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGt                   >  2:1398958/1‑139 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACag                                                                                                             <  2:683405/48‑1 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACag                                                                                                             >  1:683405/1‑48 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGtt                                                                         >  2:764844/1‑84 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGtt                                                                         <  1:764844/84‑1 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGAt                                                    >  1:910738/1‑105 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGAt                                                    <  2:910738/105‑1 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATa                                             <  1:162113/112‑1 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatg                                 >  2:221088/1‑124 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatg                                 <  1:221088/124‑1 (MQ=255)                                                                                                                  aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGCCTGCGTTTGCGCGATACCAATa                                             >  2:162113/1‑112 (MQ=255)                                                                                                                   aCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATAcc                                                                                              <  2:1032866/62‑1 (MQ=255)                                                                                                                   aCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATAcc                                                                                              >  1:1032866/1‑62 (MQ=255)                                                                                                                   aCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAg                                          <  2:1071405/114‑1 (MQ=255)                                                                                                                   aCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAg                                          >  1:1071405/1‑114 (MQ=255)                                                                                                                   aCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTAc                 >  1:15707/1‑139 (MQ=255)                                                                                                                   aCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTAc                 >  1:403097/1‑139 (MQ=255)                                                                                                                    cGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACag                                                                                                             <  1:420853/46‑1 (MQ=255)                                                                                                                    cGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACag                                                                                                             >  2:420853/1‑46 (MQ=255)                                                                                                                    cGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCg                                                                                                   >  2:454132/1‑56 (MQ=255)                                                                                                                    cGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCg                                                                                                   <  1:454132/56‑1 (MQ=255)                                                                                                                    cGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTgcgc                                                       <  1:1333141/100‑1 (MQ=255)                                                                                                                    cGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTgcgc                                                       >  2:1333141/1‑100 (MQ=255)                                                                                                                       ccGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGtt                                                            <  1:105714/92‑1 (MQ=255)                                                                                                                       ccGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGtt                                                            >  2:105714/1‑92 (MQ=255)                                                                                                                       ccGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCaaa             >  2:539463/1‑139 (MQ=255)                                                                                                                          cAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAg                                                                       <  2:44129/78‑1 (MQ=255)                                                                                                                          cAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAg                                                                       >  1:44129/1‑78 (MQ=255)                                                                                                                           aGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGa         <  1:751798/139‑1 (MQ=255)                                                                                                                                  gCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCaacga  <  1:539463/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        GCGGCGCTGGCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGA  >  NC_000913/3151293‑3151560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑