Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,308	G→A	S121L (TCG→TTG)	ftsP ←	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,308	0	G	A	100.0%	137.8 / NA	44	S121L (TCG→TTG)	ftsP	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGT  >  NC_000913/3162177‑3162438                                                                                                                                    |                                                                                                                                   cTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAAcatcat                                                                                                                             <  1:996536/139‑1 (MQ=255)                     gCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCa                                                                                                         >  1:140832/1‑139 (MQ=255)                           ccGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGc                                                                                                   <  2:454760/139‑1 (MQ=255)                               tataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               <  2:932055/139‑1 (MQ=255)                               tataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               <  2:1098587/139‑1 (MQ=255)                               tataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               <  2:496470/139‑1 (MQ=255)                                    aCCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCt                                                                                          >  2:640241/1‑139 (MQ=255)                                        gctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAg                                                                                      >  2:13858/1‑139 (MQ=255)                                         ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               <  2:62669/129‑1 (MQ=255)                                         ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTgg                                                                                               >  1:62669/1‑129 (MQ=255)                                         ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTg                                                                                                >  2:561578/1‑128 (MQ=255)                                         ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTg                                                                                                <  1:561578/128‑1 (MQ=255)                                         ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc                                                                                     >  2:219502/1‑139 (MQ=255)                                         ctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGc                                                                                     >  2:886973/1‑139 (MQ=255)                                          tgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGcc                                                                                    <  2:1119055/139‑1 (MQ=255)                                              ccGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGc                                                                                >  2:12270/1‑139 (MQ=255)                                                        gggAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGATGCCCAGTCAGCGTTTGGCAACATCGTCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCAtt                                                                      <  2:855000/139‑1 (MQ=255)                                                            gTATTGGCGTGATACCACAGACTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTCGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATCgaga                                                                  <  2:832014/139‑1 (MQ=255)                                                               ttGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACAt                                                               >  1:853232/1‑139 (MQ=255)                                                                       aTACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTc                                                       >  2:773502/1‑139 (MQ=255)                                                                                agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                >  1:1119578/1‑137 (MQ=255)                                                                                agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                <  2:1119578/137‑1 (MQ=255)                                                                                agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTg                                              >  2:131291/1‑139 (MQ=255)                                                                                agTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTg                                              >  1:746539/1‑139 (MQ=255)                                                                                 gTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTTATTGAGACATTTTCTGTCAGGCGGTTGc                                             <  1:886973/139‑1 (MQ=255)                                                                                  tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTga                                                                    <  1:1413954/115‑1 (MQ=255)                                                                                  tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTga                                                                    >  2:1413954/1‑115 (MQ=255)                                                                                  tAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCt                                            >  1:311613/1‑139 (MQ=255)                                                                                     cTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTa                                         >  2:768248/1‑139 (MQ=255)                                                                                      tGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTaa                                        <  1:639381/139‑1 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAAt                                      <  2:853232/139‑1 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAAt                                      <  2:17324/139‑1 (MQ=255)                                                                                        cGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAAt                                      <  1:640241/139‑1 (MQ=255)                                                                                          ttCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                <  2:68988/127‑1 (MQ=255)                                                                                          ttCTGGCGAATGGGCAGCACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGt                                                >  1:68988/1‑127 (MQ=255)                                                                                              ggCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCtt                                <  1:12270/139‑1 (MQ=255)                                                                                                         aGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGc                                                                                                   <  2:1027323/61‑1 (MQ=255)                                                                                                         aGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGc                                                                                                   >  1:1027323/1‑61 (MQ=255)                                                                                                                     cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAg                                                                                                        >  2:1340765/1‑44 (MQ=255)                                                                                                                     cAGTCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAg                                                                                                        <  1:1340765/44‑1 (MQ=255)                                                                                                                        tCAGCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGa      >  2:354039/1‑139 (MQ=255)                                                                                                                           gCGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGTTGTAAATAAGCTTAACATCGTCGCTCTTCCAGACGCGGATAg   >  2:856612/1‑139 (MQ=255)                                                                                                                            cGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGTTGTAAATAAGCTTAACATCGTCGCTCTTCCAGACGCGGATAGt  <  1:856612/139‑1 (MQ=255)                                                                                                                            cGTTTGGCAACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGt  <  2:140832/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGT  >  NC_000913/3162177‑3162438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑