Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,205,324	C→T	*311* (TAG→TAA)	ttdR ←	transcriptional activator of ttdABT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,205,324	0	C	T	100.0%	186.6 / NA	58	*311* (TAG→TAA)	ttdR	transcriptional activator of ttdABT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/28);  total (30/28)

GTTTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGCTT  >  NC_000913/3205189‑3205456                                                                                                                                        |                                                                                                                                     gTTTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTAc                                                                                                                                   <  2:821874/139‑1 (MQ=255)    tCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATg                                                                                                                                <  2:495702/139‑1 (MQ=255)                    tGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                <  2:550102/139‑1 (MQ=255)                           gctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCtt                                                                                                         >  2:108450/1‑139 (MQ=255)                            ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTg                                                                                                        >  2:978703/1‑139 (MQ=255)                            ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTg                                                                                                        >  2:131105/1‑139 (MQ=255)                                 tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                  >  2:136399/1‑108 (MQ=255)                                 tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                  <  1:136399/108‑1 (MQ=255)                                 tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAg                                                                                                   >  2:1388915/1‑139 (MQ=255)                                 tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAg                                                                                                   >  1:722329/1‑139 (MQ=255)                                 tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATGCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAg                                                                                                   >  1:45296/1‑139 (MQ=255)                                   atcatGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc                                                                                                 <  1:108450/139‑1 (MQ=255)                                    tcatGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCg                                                                                                <  2:45296/139‑1 (MQ=255)                                           aacaTCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTgg                                                                                         >  1:148821/1‑139 (MQ=255)                                             caTCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCa                                                                                       >  1:235497/1‑139 (MQ=255)                                               tCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCAcc                                                                                     >  1:1091628/1‑139 (MQ=255)                                               tCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCAcc                                                                                     >  2:746734/1‑139 (MQ=255)                                                 cAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCAt                                                                                   >  2:844198/1‑139 (MQ=255)                                                        tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCa                                                                            >  1:222723/1‑139 (MQ=255)                                                        tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCa                                                                            >  2:1158994/1‑139 (MQ=255)                                                         gtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAg                                                                           <  2:235497/139‑1 (MQ=255)                                                             cgtcgtCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCCGCCGTTGCTGGCACCATGCCGCCAGAAAt                                                                       >  1:1387214/1‑139 (MQ=255)                                                                 gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                  >  1:1202626/1‑76 (MQ=255)                                                                 gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                  <  2:1202626/76‑1 (MQ=255)                                                                 gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCa                                                                   <  1:709584/139‑1 (MQ=255)                                                                 gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCa                                                                   >  2:606763/1‑139 (MQ=255)                                                                 gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCa                                                                   >  2:1223526/1‑139 (MQ=255)                                                                  tcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTTTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                >  2:293146/1‑93 (MQ=255)                                                                  tcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTTTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                <  1:293146/93‑1 (MQ=255)                                                                   cAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACg                                                                 <  1:1223526/139‑1 (MQ=255)                                                                       agaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGa                                                             <  2:1091628/139‑1 (MQ=255)                                                                       agaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGa                                                             <  2:722329/139‑1 (MQ=255)                                                                        gaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGac                                                            <  2:222723/139‑1 (MQ=255)                                                                         aCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGaca                                                           >  1:405501/1‑139 (MQ=255)                                                                         aCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGaca                                                           >  1:277158/1‑139 (MQ=255)                                                                           aaaaaTCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACg                                                         <  1:1388915/139‑1 (MQ=255)                                                                           aaaaaTCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACg                                                         <  1:746734/139‑1 (MQ=255)                                                                                 cTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAAttt                                                   <  2:148821/139‑1 (MQ=255)                                                                                     aCGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               <  1:606763/139‑1 (MQ=255)                                                                                     aCGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               <  1:844198/139‑1 (MQ=255)                                                                                     aCGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               >  1:657613/1‑139 (MQ=255)                                                                                      cGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtgc                                              <  2:177424/139‑1 (MQ=255)                                                                                                aagaaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATagag                                    <  1:1158994/139‑1 (MQ=255)                                                                                                 agaaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTG‑CCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCg                                  <  1:131105/139‑1 (MQ=255)                                                                                                     agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCt                               >  2:201439/1‑139 (MQ=255)                                                                                                       cgcgAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTcc                             >  2:549227/1‑139 (MQ=255)                                                                                                        gcgAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTccc                            >  2:115814/1‑139 (MQ=255)                                                                                                              aGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                      <  1:841485/139‑1 (MQ=255)                                                                                                               ggATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAAc                     <  2:1292470/139‑1 (MQ=255)                                                                                                                 aTCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtgc                                              <  2:34558/112‑1 (MQ=255)                                                                                                                 aTCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtgc                                              >  1:34558/1‑112 (MQ=255)                                                                                                                        gTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                <  1:15881/39‑1 (MQ=255)                                                                                                                        gTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCAt                                                                                                                >  2:15881/1‑39 (MQ=255)                                                                                                                           aTTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               >  1:507674/1‑101 (MQ=255)                                                                                                                           aTTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                               <  2:507674/101‑1 (MQ=255)                                                                                                                                  gtggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGCtt  >  2:567109/1‑139 (MQ=255)                                                                                                                                   tggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCa                                                               <  2:1036053/77‑1 (MQ=255)                                                                                                                                   tggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCa                                                               >  1:1036053/1‑77 (MQ=255)                                                                                                                                        |                                                                                                                                     GTTTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGCTT  >  NC_000913/3205189‑3205456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑