Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,309,007 G→A intergenic (‑83/+26) nlpI ← / ← pnp lipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,309,0070GA100.0% 109.6 / NA 35intergenic (‑83/+26)nlpI/pnplipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/17);  total (18/17)

TAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCC  >  NC_000913/3308882‑3309122
                                                                                                                             |                                                                                                                   
tAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGc                                                                                                       >  1:1161658/1‑139 (MQ=255)
  aGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCaa                                                                                                     >  1:198147/1‑139 (MQ=255)
       tGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGc                                                                                                >  2:115084/1‑139 (MQ=255)
               cGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                        <  1:115084/139‑1 (MQ=255)
                          cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTc                                                                             >  1:277709/1‑139 (MQ=255)
                           gcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTca                                                                            >  2:1412068/1‑139 (MQ=255)
                                    gCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGa                                                                   <  2:198147/139‑1 (MQ=255)
                                           ttCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATgg                                                                                                 <  1:157183/102‑1 (MQ=255)
                                           ttCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATgg                                                                                                 >  2:157183/1‑102 (MQ=255)
                                                    ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                   <  1:103809/139‑1 (MQ=255)
                                                         gACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGt                                              <  1:743163/139‑1 (MQ=255)
                                                            cACGGTTGAATGAACGTGCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCACAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                                           >  1:892963/1‑139 (MQ=255)
                                                            cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATgg                                                                                                 >  1:1076694/1‑85 (MQ=255)
                                                            cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATgg                                                                                                 <  2:1076694/85‑1 (MQ=255)
                                                            cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAAc                                                                                             >  1:506250/1‑89 (MQ=255)
                                                            cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAAc                                                                                             <  2:506250/89‑1 (MQ=255)
                                                            cACGGTTGAATGAACGTCCTGTACCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTcc                                                             <  2:1410120/121‑1 (MQ=255)
                                                            cACGGTTGAATGAACGTCCTGTACCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTcc                                                             >  1:1410120/1‑121 (MQ=255)
                                                              cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGc                                                                                                >  1:728448/1‑84 (MQ=255)
                                                              cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGc                                                                                                <  2:728448/84‑1 (MQ=255)
                                                                           gTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                   <  1:623081/84‑1 (MQ=255)
                                                                           gTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                   >  2:623081/1‑84 (MQ=255)
                                                                              cTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                >  1:220809/1‑132 (MQ=255)
                                                                              cTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                <  2:220809/132‑1 (MQ=255)
                                                                                  tCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTc                                                              <  1:836869/98‑1 (MQ=255)
                                                                                  tCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTc                                                              >  2:836869/1‑98 (MQ=255)
                                                                                     cGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                   <  2:181282/106‑1 (MQ=255)
                                                                                     cGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                   >  1:181282/1‑106 (MQ=255)
                                                                                      ggTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCt                 <  2:892963/139‑1 (MQ=255)
                                                                                          gCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGa             <  1:485781/139‑1 (MQ=255)
                                                                                           cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGAc            >  1:919664/1‑139 (MQ=255)
                                                                                                aaGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATa       <  1:758177/139‑1 (MQ=255)
                                                                                                     gTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGcc  >  1:499720/1‑139 (MQ=255)
                                                                                                                          cccACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                                           >  2:478634/1‑78 (MQ=255)
                                                                                                                          cccACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                                           <  1:478634/78‑1 (MQ=255)
                                                                                                                             |                                                                                                                   
TAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCC  >  NC_000913/3308882‑3309122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: