Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 323,355 C→T P340S (CCA→TCA)  ykgF → ferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913323,3550CT100.0% 146.9 / NA 48P340S (CCA→TCA) ykgFferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/24);  total (24/24)

TGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAA  >  NC_000913/323249‑323477
                                                                                                          |                                                                                                                          
tGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                            <  2:1090792/139‑1 (MQ=255)
tGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                            >  2:1205415/1‑139 (MQ=255)
                      tGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                      <  2:1411923/139‑1 (MQ=255)
                                gCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                                    <  2:712502/115‑1 (MQ=255)
                                gCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                                    >  1:712502/1‑115 (MQ=255)
                                  ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                            >  1:1064257/1‑89 (MQ=255)
                                  ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                            <  2:1064257/89‑1 (MQ=255)
                                  ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                                    >  1:141128/1‑113 (MQ=255)
                                  ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                                    <  2:141128/113‑1 (MQ=255)
                                  ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                          <  1:1205415/139‑1 (MQ=255)
                                  ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                          >  1:929777/1‑139 (MQ=255)
                                              aaTACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggcgg                                                                                          <  2:1086126/95‑1 (MQ=255)
                                              aaTACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTCCGGTGATTTCTCCGCTACTTggcgg                                                                                          >  1:1086126/1‑95 (MQ=255)
                                                  cTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                                          >  1:407782/1‑139 (MQ=255)
                                                    tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                      >  1:957886/1‑109 (MQ=255)
                                                    tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                      <  2:957886/109‑1 (MQ=255)
                                                     gTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTtctc                                                                                                       <  1:763861/75‑1 (MQ=255)
                                                     gTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTtctc                                                                                                       >  2:763861/1‑75 (MQ=255)
                                                     gTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                                    >  2:180039/1‑94 (MQ=255)
                                                     gTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                                    <  1:180039/94‑1 (MQ=255)
                                                         ggCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                            >  2:880153/1‑66 (MQ=255)
                                                         ggCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                            <  1:880153/66‑1 (MQ=255)
                                                                cGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgt                            >  1:633808/1‑139 (MQ=255)
                                                                     tatTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCg                       <  2:633808/139‑1 (MQ=255)
                                                                          gCGGTCATGGATATGGCTCTATTTATCCAGGGTCTATTGGTGCGGTGAt                                                                                                            >  2:1107273/1‑49 (MQ=255)
                                                                          gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                            <  1:1107273/49‑1 (MQ=255)
                                                                          gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtat                                                                                      <  1:1120749/71‑1 (MQ=255)
                                                                          gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtat                                                                                      >  2:1120749/1‑71 (MQ=255)
                                                                          gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGc                                                            <  2:998052/97‑1 (MQ=255)
                                                                          gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGc                                                            >  1:998052/1‑97 (MQ=255)
                                                                             gTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagat                                                                       <  1:416888/83‑1 (MQ=255)
                                                                             gTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagat                                                                       >  2:416888/1‑83 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                            >  2:1270464/1‑35 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                            <  1:242555/35‑1 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                            >  2:242555/1‑35 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                            <  1:1270464/35‑1 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc                        >  1:369858/1‑119 (MQ=255)
                                                                                        ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc                        <  2:369858/119‑1 (MQ=255)
                                                                                          ctctTTTTATCCAGGGTCGTTTGGTGCGGTGATATCTACGCTACTGGGCGGCGATAAAGAGTTTAAAGATTTACCCGGCGCCTGCTCGTTATGCAAGTCTTGTGACAACGTGTGGCCGGTGCGTATTCCGCTGTCaaca  >  2:1399885/1‑137 (MQ=255)
                                                                                          ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaaaa  <  2:929777/139‑1 (MQ=255)
                                                                                             tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                             >  2:1332242/1‑45 (MQ=255)
                                                                                             tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                             <  1:1332242/45‑1 (MQ=255)
                                                                                             tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                                          >  1:976373/1‑96 (MQ=255)
                                                                                             tATTTATCCAGGGTCAATTGGTGCGGTGATGTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                                          <  2:976373/96‑1 (MQ=255)
                                                                                                       gggTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                             <  2:1080195/35‑1 (MQ=255)
                                                                                                       gggTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                             >  1:1080195/1‑35 (MQ=255)
                                                                                                       gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                        >  2:1252206/1‑72 (MQ=255)
                                                                                                       gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                        <  1:1252206/72‑1 (MQ=255)
                                                                                                          |                                                                                                                          
TGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAA  >  NC_000913/323249‑323477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: