| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,457,718 | A→G | I448V (ATC→GTC) | gspD → | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,457,718 | 0 | A | G | 98.5% | 228.4 / NA | 65 | I448V (ATC→GTC) | gspD | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (33/31); minor base T (1/0); total (34/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAACGTCTTTAATACCGTCGAACGCAAAACGGTGGGGACAAA > NC_000913/3457583‑3457845 | aCCAGCGCGAATCCTGCCTGGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCg < 1:423405/139‑1 (MQ=255) cgAATCCTGCCTGGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGGTTACCGCGCTGACCAGTAACAATGAAAATGACATGCTCGCCACCCCAAGCGTCGTAACg > 2:912274/1‑137 (MQ=255) cgAATCCTGCCTGGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCt > 2:715460/1‑139 (MQ=255) cgAATCCTGCCTGGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCt > 1:762808/1‑139 (MQ=255) cgAATCCTGCCTGGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGACCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACg < 1:912274/137‑1 (MQ=255) aTCCTGCCTGGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACg > 2:1351678/1‑134 (MQ=255) aTCCTGCCTGGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACg < 1:1351678/134‑1 (MQ=255) gcctgGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGataata > 2:915289/1‑139 (MQ=255) cctgGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATAACATCCTCGCCACCCCAAGCGTCGTAACGCTg < 1:1391163/131‑1 (MQ=255) cctgGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATAACATCCTCGCCACCCCAAGCGTCGTAACGCTg > 2:1391163/1‑131 (MQ=255) tatGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTcgcg < 1:915289/139‑1 (MQ=255) aGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGCCTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCCCCCCAAGCGTCGTAACGCTGGATAAAAAACTCGCGTCCTTCa > 2:808921/1‑139 (MQ=255) gcaccTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCaa > 1:428178/4‑139 (MQ=255) ccTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGt < 2:762808/139‑1 (MQ=255) cTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACg > 2:1335023/1‑109 (MQ=255) cTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACg < 1:1335023/109‑1 (MQ=255) aCAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTggg > 1:1207058/1‑139 (MQ=255) aCAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTggg > 1:837435/1‑139 (MQ=255) atggcatggcCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAATTCGCGTCCTTCAACGTGGGGCa < 1:715460/139‑1 (MQ=255) ggcCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTATCAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTcg > 1:110330/1‑113 (MQ=255) ggcCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTcg < 2:110330/113‑1 (MQ=255) gcCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGc < 2:1207058/139‑1 (MQ=255) gcCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGc < 2:53644/139‑1 (MQ=255) gcCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGc < 2:837435/139‑1 (MQ=255) ttcttcAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTAt < 1:808921/139‑1 (MQ=255) cttcAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATcc > 1:781915/1‑139 (MQ=255) tGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCg < 1:687060/124‑1 (MQ=255) tGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCg > 2:687060/1‑124 (MQ=255) gACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAA‑GTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGAc < 2:428178/139‑1 (MQ=255) aCTGGGGAGTACTGCTTACCGCGCTGGCCAGTATCAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTg < 1:86698/77‑1 (MQ=255) aCTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTg > 2:86698/1‑77 (MQ=255) gggAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCg > 1:111048/1‑65 (MQ=255) gggAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCg < 2:111048/65‑1 (MQ=255) tGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTg < 1:262168/110‑1 (MQ=255) tGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTg > 2:262168/1‑110 (MQ=255) cTTACCGGGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACg > 2:937233/1‑60 (MQ=255) cTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACg < 1:937233/60‑1 (MQ=255) cTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGa < 1:1292682/65‑1 (MQ=255) cTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGa > 2:1292682/1‑65 (MQ=255) cTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATa > 1:51139/1‑139 (MQ=255) tACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAAc < 2:1073421/139‑1 (MQ=255) cgcgCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGa < 2:1112211/93‑1 (MQ=255) cgcgCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGa > 1:1112211/1‑93 (MQ=255) cgcgCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAACGTc > 2:723272/1‑139 (MQ=255) cgcTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAACGTCtt > 2:570407/1‑139 (MQ=255) gcTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGAc > 1:1148227/1‑117 (MQ=255) gcTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGAc < 2:1148227/117‑1 (MQ=255) tGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTc < 2:993631/121‑1 (MQ=255) tGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTc > 1:993631/1‑121 (MQ=255) aTAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCgg < 2:1013959/84‑1 (MQ=255) aTAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCgg > 1:1013959/1‑84 (MQ=255) aTAAAAATGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATCACGTCTTTAATACCGTCGAACg > 2:932109/1‑139 (MQ=255) aaTGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGAc < 2:416777/98‑1 (MQ=255) aaTGACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGAc > 1:416777/1‑98 (MQ=255) gACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAACGTCTTTAATACCGTCg > 2:209129/1‑127 (MQ=255) gACATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAACGTCTTTAATACCGTCg < 1:209129/127‑1 (MQ=255) aCATCCTCGCCACCCCAAGCTTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAACGTCTTTAATa > 2:840522/1‑120 (MQ=255) aCATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCg > 2:58568/1‑84 (MQ=255) aCATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCg < 1:58568/84‑1 (MQ=255) aCATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTca > 2:501802/1‑89 (MQ=255) aCATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTca < 1:501802/89‑1 (MQ=255) aCATCCTCGCCACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAACGTCTTTAATa < 1:840522/120‑1 (MQ=255) ccACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGAGGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAACGCCTTTAATACCGTCTAACGCAAAACGGTAAGGACaaa > 1:1017864/1‑139 (MQ=255) cACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGAc > 2:348976/1‑84 (MQ=255) cACCCCAAGCGTCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGAc < 1:348976/84‑1 (MQ=255) | ACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCTTCAACGTGGGGCAGGATGTGCCGGTGCTATCCGGGTCACAGACCACTTCAGGGGATAACGTCTTTAATACCGTCGAACGCAAAACGGTGGGGACAAA > NC_000913/3457583‑3457845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |