Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	335,970	C→T	Q16* (CAA→TAA)	yahE →	DUF2877 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	335,970	0	C	T	100.0%	259.0 / NA	79	Q16* (CAA→TAA)	yahE	DUF2877 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/39);  total (40/39)

GGAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACT  >  NC_000913/335834‑336099                                                                                                                                         |                                                                                                                                  ggAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACG‑‑GTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                               <  2:840104/139‑1 (MQ=255)        ggCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAgg                                                                                                                          <  1:997135/139‑1 (MQ=255)              gAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                    <  2:858805/139‑1 (MQ=255)              gAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                    <  1:247137/139‑1 (MQ=255)                   gaTTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTc                                                                                                               >  1:1137350/1‑139 (MQ=255)                    aTTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCt                                                                                                              >  1:218869/1‑139 (MQ=255)                      tGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCttt                                                                                                            >  1:1375861/1‑139 (MQ=255)                           aGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCag                                                                                                       <  2:418697/139‑1 (MQ=255)                            gTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                      <  2:1375861/139‑1 (MQ=255)                            gTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                      <  2:218869/139‑1 (MQ=255)                               aCTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCg                                                                                                   >  1:532407/1‑139 (MQ=255)                                     tGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAat                                                                                             >  1:1239078/1‑139 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        <  2:36990/126‑1 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        >  1:36990/1‑126 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                           >  1:18953/1‑139 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                           <  2:1239078/139‑1 (MQ=255)                                         gCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCg                                                                                         <  1:954275/139‑1 (MQ=255)                                         gCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCg                                                                                         <  1:945958/139‑1 (MQ=255)                                             gTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                      <  2:422368/122‑1 (MQ=255)                                             gTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                      >  1:422368/1‑122 (MQ=255)                                                  tAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCgg                                                                                <  1:330720/139‑1 (MQ=255)                                                         ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGATCGGTAAATATCGCACTCCCGGCTCGCca                                                                         >  2:675952/1‑139 (MQ=255)                                                         ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCca                                                                         >  1:797256/1‑139 (MQ=255)                                                         ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCca                                                                         >  2:1341994/1‑139 (MQ=255)                                                          gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCcag                                                                        >  2:789770/1‑139 (MQ=255)                                                               ttGCTATCAACACACCAGAAAGACGGTGGGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGtt                                                                   >  1:748666/1‑139 (MQ=255)                                                                        acacacCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                          <  2:797256/139‑1 (MQ=255)                                                                           cacCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgct                                                       <  1:789770/139‑1 (MQ=255)                                                                            acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        <  1:405243/89‑1 (MQ=255)                                                                            acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        >  2:405243/1‑89 (MQ=255)                                                                            acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCtt                                                      >  1:303137/1‑139 (MQ=255)                                                                            acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCtt                                                      >  2:439838/1‑139 (MQ=255)                                                                               aGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                               <  2:1195682/63‑1 (MQ=255)                                                                               aGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                               >  1:1195682/1‑63 (MQ=255)                                                                                gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                  <  1:367756/139‑1 (MQ=255)                                                                                gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                                  <  2:1137350/139‑1 (MQ=255)                                                                                      cGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAgg                                                                                                                          >  2:403455/1‑61 (MQ=255)                                                                                      cGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAgg                                                                                                                          <  1:403455/61‑1 (MQ=255)                                                                                        gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCg                                                                                         >  1:1085511/1‑92 (MQ=255)                                                                                        gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCg                                                                                         >  2:1101973/1‑92 (MQ=255)                                                                                        gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCg                                                                                         <  2:1085511/92‑1 (MQ=255)                                                                                        gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCg                                                                                         <  1:1101973/92‑1 (MQ=255)                                                                                         tgtgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        >  1:754594/1‑76 (MQ=255)                                                                                         tgtgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        <  2:754594/76‑1 (MQ=255)                                                                                             tgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACaa                                     >  2:1043665/1‑139 (MQ=255)                                                                                               tgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                                >  1:626777/1‑126 (MQ=255)                                                                                               tgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                                <  2:626777/126‑1 (MQ=255)                                                                                                   cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGc                                                                                                          <  2:72450/64‑1 (MQ=255)                                                                                                   cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGc                                                                                                          >  1:72450/1‑64 (MQ=255)                                                                                                   cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        >  1:851329/1‑66 (MQ=255)                                                                                                   cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        <  2:851329/66‑1 (MQ=255)                                                                                                   cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACa                                      <  2:418353/132‑1 (MQ=255)                                                                                                   cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACa                                      >  1:418353/1‑132 (MQ=255)                                                                                                     cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        >  1:472189/1‑64 (MQ=255)                                                                                                     cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        <  2:472189/64‑1 (MQ=255)                                                                                                     cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaa                             >  1:1348850/1‑139 (MQ=255)                                                                                                      tAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaaa                            <  2:532407/139‑1 (MQ=255)                                                                                                      tAACAGCGGAAGCGGATTTTCTGGCGCGGCGGGGGTAAGGACAGGTTGAGCGGGTGTTGGCGAGAGCGGTAAATATCGCACTTCCGGGGCGCCGGCATTTGCTGACGCTGCTTAGTGAAGAGTACGAAAATGCGCGaaa                            <  2:762378/139‑1 (MQ=255)                                                                                                       aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGATCAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCTCCAGCAGTTGCTGACg                                                            <  2:312956/106‑1 (MQ=255)                                                                                                       aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACg                                                            >  1:312956/1‑106 (MQ=255)                                                                                                       aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAAc                           >  1:737915/1‑139 (MQ=255)                                                                                                       aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAAc                           >  2:218480/1‑139 (MQ=255)                                                                                                       aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGATGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAAc                           >  2:1009875/1‑139 (MQ=255)                                                                                                           gcggatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGtt                       >  1:691993/1‑139 (MQ=255)                                                                                                            cggatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTg                      <  2:748666/139‑1 (MQ=255)                                                                                                            cggatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTg                      <  1:1043665/139‑1 (MQ=255)                                                                                                              gatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        >  1:1264821/1‑55 (MQ=255)                                                                                                              gatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                        <  2:1264821/55‑1 (MQ=255)                                                                                                                tgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCgg                  >  2:640819/1‑139 (MQ=255)                                                                                                                  cggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGtt                >  1:488061/1‑139 (MQ=255)                                                                                                                  cggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGtt                >  1:988472/1‑139 (MQ=255)                                                                                                                    gatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTgg              <  2:737915/139‑1 (MQ=255)                                                                                                                    gatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTgg              >  1:1218059/1‑139 (MQ=255)                                                                                                                            ggCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACATTGCGCCAAACAGTTGTCGGTTGGcactcact      <  2:303137/139‑1 (MQ=255)                                                                                                                            ggCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcact      <  2:81779/139‑1 (MQ=255)                                                                                                                               gcAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCgg                  <  1:1290905/124‑1 (MQ=255)                                                                                                                               gcAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCgg                  >  2:1290905/1‑124 (MQ=255)                                                                                                                               gcAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcactcac   >  2:720073/1‑139 (MQ=255)                                                                                                                                cAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcactcact  <  2:1277706/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GGAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCACT  >  NC_000913/335834‑336099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑