| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,501,096 | T→C | L396P (CTC→CCC) | frlA → | putative fructoselysine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,501,096 | 0 | T | C | 97.0% | 227.6 / ‑0.5 | 66 | coding (1187/1338 nt) | frlA | putative fructoselysine transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (35/29); minor base . (1/1); total (36/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 | |||||||||||
CCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGCGTAGCCGC > NC_000913/3500965‑3501226 | ccTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCAtt > 2:1428030/1‑139 (MQ=255) tgGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc < 1:1428030/139‑1 (MQ=255) tATTTCACCCTAGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGcc < 1:927647/139‑1 (MQ=255) tCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCAt > 2:865823/1‑139 (MQ=255) tttCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAAcc > 2:165611/1‑139 (MQ=255) aCCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGa > 2:1092114/1‑139 (MQ=255) ccTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt < 1:1129799/117‑1 (MQ=255) ccTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt > 2:1129799/1‑117 (MQ=255) tCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTcc > 2:6088/1‑139 (MQ=255) cGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAg < 2:1266978/96‑1 (MQ=255) cGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAg > 1:1266978/1‑96 (MQ=255) gCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt < 1:510367/111‑1 (MQ=255) gCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt > 2:510367/1‑111 (MQ=255) atcatcTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt < 2:473469/106‑1 (MQ=255) atcatcTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt > 1:473469/1‑106 (MQ=255) atcatcTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAAcc > 2:628020/1‑111 (MQ=255) atcatcTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAAcc < 1:628020/111‑1 (MQ=255) tGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTgcg < 1:6088/139‑1 (MQ=255) gtgtCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGcc < 1:865823/139‑1 (MQ=255) gtCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGt < 1:1092114/139‑1 (MQ=255) tCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCAcc > 2:359577/1‑114 (MQ=255) tCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCAcc < 1:359577/114‑1 (MQ=255) tCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTc > 2:1096368/1‑139 (MQ=255) tCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTc < 1:1096368/139‑1 (MQ=255) tCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTc < 1:165611/139‑1 (MQ=255) tAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc > 2:361508/1‑72 (MQ=255) tAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc < 1:361508/72‑1 (MQ=255) tAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATc < 2:1370585/139‑1 (MQ=255) tAAACGCGACGATTACAAACCGCTGTGGCGTAATCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTCTTTCTTGGCACCGATTCCCGGCCTTATCTCCGCCGTCacc > 2:250294/1‑137 (MQ=255) cgcgACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc > 2:1417940/1‑68 (MQ=255) cgcgACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc < 1:1417940/68‑1 (MQ=255) cgcgACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTTGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATc > 1:758599/1‑135 (MQ=255) cgcgACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTc > 1:453488/1‑112 (MQ=255) cgcgACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTc < 2:453488/112‑1 (MQ=255) cgcgACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATc < 2:758599/135‑1 (MQ=255) gcgACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTAt < 1:474876/139‑1 (MQ=255) gacgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc < 2:1367156/65‑1 (MQ=255) gacgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc > 1:1367156/1‑65 (MQ=255) gacgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGtt > 2:1133731/1‑135 (MQ=255) gacgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGtt < 1:1133731/135‑1 (MQ=255) acgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCCTTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGc > 2:1131878/1‑139 (MQ=255) acgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCC‑CGCCa < 2:537311/56‑1 (MQ=255) acgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCC‑CGCCa > 1:537311/1‑56 (MQ=255) cgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTAt > 1:1406463/1‑117 (MQ=255) cgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTAt < 2:1406463/117‑1 (MQ=255) cgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCt < 2:483203/139‑1 (MQ=255) cTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCtt < 2:1010404/77‑1 (MQ=255) cTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCtt > 1:1010404/1‑77 (MQ=255) ggCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTa > 2:1296264/1‑139 (MQ=255) tACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGcc > 2:503894/1‑139 (MQ=255) cTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGc > 1:1046848/1‑44 (MQ=255) cTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGc < 2:1046848/44‑1 (MQ=255) gCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTggg > 1:518831/1‑139 (MQ=255) gCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTggg > 1:374952/1‑139 (MQ=255) ttCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGa > 2:1268537/1‑139 (MQ=255) tCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGaa > 2:456386/1‑139 (MQ=255) gggCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTc < 2:33259/93‑1 (MQ=255) gggCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTc > 1:33259/1‑93 (MQ=255) gggCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGc > 1:1373994/1‑139 (MQ=255) gCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTAt > 1:534878/1‑99 (MQ=255) gCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTAt < 2:534878/99‑1 (MQ=255) cTGATGACCACCCCCGCCATCGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGCGTa > 1:1055302/1‑139 (MQ=255) gatgaCCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGaa > 1:996911/1‑132 (MQ=255) gatgaCCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGaa < 2:996911/132‑1 (MQ=255) gatgaCCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGCGTAgc > 2:1228044/1‑139 (MQ=255) gaCCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGCGTAgccgc < 1:695256/139‑1 (MQ=255) | CCTGCTGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGCGTAGCCGC > NC_000913/3500965‑3501226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |