Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,516,104 T→C K401K (AAA→AAG)  damX ← cell division protein that binds to the septal ring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,516,1040TC96.8% 219.5 / ‑0.4 63coding (1203/1287 nt)damXcell division protein that binds to the septal ring
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (29/32);  minor base . (1/1);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

CGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAG  >  NC_000913/3515970‑3516226
                                                                                                                                      |                                                                                                                          
cgcTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCctttt                                                                                                                        >  1:239328/1‑139 (MQ=255)
                      gATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTa                                                                                                  <  1:1119563/139‑1 (MQ=255)
                             cATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           >  1:627262/1‑139 (MQ=255)
                               ttGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGc                                                                                                                    >  1:35819/1‑112 (MQ=255)
                               ttGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGc                                                                                                                    <  2:35819/112‑1 (MQ=255)
                               ttGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGa                                                                                         >  2:701566/1‑139 (MQ=255)
                                tGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaa                                                                                        >  2:365057/1‑139 (MQ=255)
                                  cGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                      <  1:701566/139‑1 (MQ=255)
                                       gaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                 >  2:816284/1‑139 (MQ=255)
                                       gaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                 <  1:816284/139‑1 (MQ=255)
                                       gaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                 <  2:239328/139‑1 (MQ=255)
                                           aCCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATa                                                                             <  2:1415157/139‑1 (MQ=255)
                                                 aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           >  1:169955/1‑119 (MQ=255)
                                                 aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           <  2:169955/119‑1 (MQ=255)
                                                 aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACgg                                                                       >  1:1075935/1‑139 (MQ=255)
                                                 aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACgg                                                                       >  1:605960/1‑139 (MQ=255)
                                                    aCTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTg                                                                    <  2:605960/139‑1 (MQ=255)
                                                     cTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGa                                                                   <  1:365057/139‑1 (MQ=255)
                                                           aTCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTa                                                             >  2:206554/1‑139 (MQ=255)
                                                             cGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcg                                                           <  2:627262/139‑1 (MQ=255)
                                                               gCCTGTACCTGACGCAGCGGTCTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                         <  1:206554/139‑1 (MQ=255)
                                                                         gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           >  1:714578/1‑95 (MQ=255)
                                                                         gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           <  2:477634/95‑1 (MQ=255)
                                                                         gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           <  2:1402202/95‑1 (MQ=255)
                                                                         gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           >  1:1402202/1‑95 (MQ=255)
                                                                         gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           >  1:477634/1‑95 (MQ=255)
                                                                         gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           <  2:714578/95‑1 (MQ=255)
                                                                          aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           >  1:239876/1‑94 (MQ=255)
                                                                          aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           <  2:239876/94‑1 (MQ=255)
                                                                          aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                      <  1:411541/99‑1 (MQ=255)
                                                                          aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                      >  2:411541/1‑99 (MQ=255)
                                                                          aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATa                                               <  2:803272/138‑1 (MQ=255)
                                                                          aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATa                                               >  1:803272/1‑138 (MQ=255)
                                                                           cGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                             <  1:766610/139‑1 (MQ=255)
                                                                              aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGcc                                                                                                                   <  2:1298117/66‑1 (MQ=255)
                                                                              aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGcc                                                                                                                   >  1:1298117/1‑66 (MQ=255)
                                                                              aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           <  2:1067825/90‑1 (MQ=255)
                                                                              aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                           >  1:1067825/1‑90 (MQ=255)
                                                                              aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaaa                                                                                       >  2:464906/1‑94 (MQ=255)
                                                                              aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaaa                                                                                       <  1:464906/94‑1 (MQ=255)
                                                                              aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGC‑CTTTTCGCctc                                                                                                                 >  2:1007260/1‑67 (MQ=255)
                                                                              aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGC‑CTTTTCGCctc                                                                                                                 <  1:1007260/67‑1 (MQ=255)
                                                                                cGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGcc                                                                                                                   <  1:1030964/64‑1 (MQ=255)
                                                                                cGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGcc                                                                                                                   >  2:1030964/1‑64 (MQ=255)
                                                                                     tCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                       <  2:135509/119‑1 (MQ=255)
                                                                                     tCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                       >  1:135509/1‑119 (MQ=255)
                                                                                            cGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                      >  2:752440/1‑81 (MQ=255)
                                                                                            cGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                      <  1:752440/81‑1 (MQ=255)
                                                                                             gggTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                      <  2:1301789/80‑1 (MQ=255)
                                                                                             gggTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                      >  1:1301789/1‑80 (MQ=255)
                                                                                              gggttttGGCCTGGACATCTGCTGGCAATGTAGATACCGCcttt                                                                                                                         <  1:645195/41‑1 (MQ=255)
                                                                                              gggttttGGCCTGGACATCTGCTGGCAATGTAGATACCGCcttt                                                                                                                         >  2:645195/4‑44 (MQ=38)
                                                                                                            aCATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGccc            <  2:1075935/139‑1 (MQ=255)
                                                                                                                ctgctgGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                         <  1:848440/90‑1 (MQ=255)
                                                                                                                ctgctgGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                         >  2:848440/1‑90 (MQ=255)
                                                                                                                      gCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAg  >  2:1149085/1‑139 (MQ=255)
                                                                                                                          tGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                         >  2:578539/1‑80 (MQ=255)
                                                                                                                          tGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                         <  1:578539/80‑1 (MQ=255)
                                                                                                                             aGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCAtt                                                              >  2:1078301/1‑72 (MQ=255)
                                                                                                                             aGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCAtt                                                              <  1:1078301/72‑1 (MQ=255)
                                                                                                                                tACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                          <  1:909248/57‑1 (MQ=255)
                                                                                                                                tACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                          >  2:909248/1‑57 (MQ=255)
                                                                                                                                tACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                       <  2:1097140/76‑1 (MQ=255)
                                                                                                                                tACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                       >  1:1097140/1‑76 (MQ=255)
                                                                                                                                tACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCAt                                                >  2:1373635/1‑83 (MQ=255)
                                                                                                                                tACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCAt                                                <  1:1373635/83‑1 (MQ=255)
                                                                                                                                      |                                                                                                                          
CGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAG  >  NC_000913/3515970‑3516226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑