Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,545,565	G→A	*228* (TAG→TAA)	gntX →	DNA catabolic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,545,565	0	G	A	100.0%	216.1 / NA	67	*228* (TAG→TAA)	gntX	DNA catabolic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/35);  total (32/35)

AAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAGAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGAAGAAGGGACACAAATCC  >  NC_000913/3545438‑3545699                                                                                                                                 |                                                                                                                                       aaGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             <  1:1283965/139‑1 (MQ=255)     tCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                                         <  1:775982/139‑1 (MQ=255)     tCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                                         <  2:194253/139‑1 (MQ=255)      cGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        <  2:665784/139‑1 (MQ=255)        ccATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                                            <  2:1400251/133‑1 (MQ=255)        ccATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                                            >  1:1400251/1‑133 (MQ=255)        ccATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        <  1:428998/137‑1 (MQ=255)        ccATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        >  2:428998/1‑137 (MQ=255)        ccATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGt                                                                                                                      >  2:279341/1‑139 (MQ=255)             tGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGtattat                                                                                                                 >  2:1234901/1‑139 (MQ=255)                tGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAAc                                                                                                              >  1:1170307/1‑139 (MQ=255)                 gATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAAcc                                                                                                             <  1:1234901/139‑1 (MQ=255)                      tgGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        >  2:358485/1‑123 (MQ=255)                      tgGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        <  1:358485/123‑1 (MQ=255)                       ggATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCg                                                                                                                                 >  1:1084563/1‑113 (MQ=255)                       ggATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCg                                                                                                                                 <  2:1084563/113‑1 (MQ=255)                                cGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCaa                                                                                              <  2:25175/139‑1 (MQ=255)                                      caccGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTa                                                                                        <  2:1170307/139‑1 (MQ=255)                                      caccGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTa                                                                                        <  2:1152148/139‑1 (MQ=255)                                         cGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  2:44177/1‑139 (MQ=255)                                         cGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     <  1:44177/139‑1 (MQ=255)                                         cGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGACGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  1:1298266/1‑139 (MQ=255)                                           gAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   <  2:1298266/139‑1 (MQ=255)                                              gTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTa                                                                                <  1:279341/139‑1 (MQ=255)                                               tACCGTCGCAGAGATTGCGCAGTTGCTTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTa                                                                                <  1:210101/139‑1 (MQ=255)                                                   gTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                                               >  2:1043320/1‑103 (MQ=255)                                                   gTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                                               <  1:1043320/103‑1 (MQ=255)                                                      gCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                                                >  2:256693/1‑115 (MQ=255)                                                      gCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                                                <  1:256693/115‑1 (MQ=255)                                                            aTTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  1:944290/1‑120 (MQ=255)                                                            aTTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     <  2:944290/120‑1 (MQ=255)                                                              tGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGcc                                                                                                                                    <  1:849180/71‑1 (MQ=255)                                                              tGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGcc                                                                                                                                    >  2:849180/1‑71 (MQ=255)                                                                cgcAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        >  1:780236/1‑81 (MQ=255)                                                                cgcAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        <  2:780236/81‑1 (MQ=255)                                                                      tGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                                         <  2:992890/74‑1 (MQ=255)                                                                      tGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                                         >  1:992890/1‑74 (MQ=255)                                                                      tGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGa                                                                          >  2:305136/1‑121 (MQ=255)                                                                      tGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGa                                                                          <  1:305136/121‑1 (MQ=255)                                                                                   aTGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACt                                            >  1:147231/1‑139 (MQ=255)                                                                                    tGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGACGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                                               <  1:279151/71‑1 (MQ=255)                                                                                    tGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGACGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                                               >  2:279151/1‑71 (MQ=255)                                                                                        gcggcgGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             <  1:1264906/53‑1 (MQ=255)                                                                                        gcggcgGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             >  2:1264906/1‑53 (MQ=255)                                                                                         cggcggCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        <  1:184151/57‑1 (MQ=255)                                                                                         cggcggCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                                        >  2:184151/1‑57 (MQ=255)                                                                                              gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTa                                                                                                        <  1:221334/68‑1 (MQ=255)                                                                                              gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTa                                                                                                        >  2:221334/1‑68 (MQ=255)                                                                                              gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATg                                                                           >  1:937435/1‑97 (MQ=255)                                                                                              gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATg                                                                           <  2:937435/97‑1 (MQ=255)                                                                                                tGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             >  1:1020990/1‑45 (MQ=255)                                                                                                tGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                                             <  2:1020990/45‑1 (MQ=255)                                                                                                    cAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCa                           >  1:1065102/1‑139 (MQ=255)                                                                                                     aGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   >  2:137742/1‑82 (MQ=255)                                                                                                     aGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   <  1:137742/82‑1 (MQ=255)                                                                                                            gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     <  1:498512/73‑1 (MQ=255)                                                                                                            gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  2:498512/1‑73 (MQ=255)                                                                                                            gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     >  2:70793/1‑73 (MQ=255)                                                                                                            gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                                     <  1:70793/73‑1 (MQ=255)                                                                                                            gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   >  1:669793/1‑75 (MQ=255)                                                                                                            gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                                   <  2:669793/75‑1 (MQ=255)                                                                                                            gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAgcg                                                <  1:1087399/110‑1 (MQ=255)                                                                                                            gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAgcg                                                >  2:1087399/1‑110 (MQ=255)                                                                                                                 tttGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGaagaag              >  2:811503/1‑139 (MQ=255)                                                                                                                             tGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATc                       <  2:1242034/118‑1 (MQ=255)                                                                                                                             tGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATc                       >  1:1242034/1‑118 (MQ=255)                                                                                                                             tGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGAAGAAGGGACACAAATcc  >  1:688171/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       AAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGC‑TTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAGAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGAAGAAGGGACACAAATCC  >  NC_000913/3545438‑3545699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑