Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,587,370	C→T	T143I (ACT→ATT)  *248* (TAG→TAA)	yhhA → ugpQ ←	DUF2756 family protein glycerophosphodiester phosphodiesterase, cytosolic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,587,370	0	C	T	100.0%	174.4 / NA	54	T143I (ACT→ATT)  *248* (TAG→TAA)	yhhA ugpQ	DUF2756 family protein glycerophosphodiester phosphodiesterase, cytosolic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/28);  total (26/28)

ACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGT  >  NC_000913/3587235‑3587500                                                                                                                                        |                                                                                                                                   acacCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTAt                                                                                                                                 >  2:1272314/1‑139 (MQ=255)            gggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTaaa                                                                                                                      >  2:116071/1‑139 (MQ=255)             ggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAg                                                                                                                     >  1:82496/1‑139 (MQ=255)                 gTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCg                                                                                                                 <  1:412975/139‑1 (MQ=255)                   tAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGa                                                                                                               <  1:1418491/139‑1 (MQ=255)                          cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGt                                                                                                                    >  1:861375/1‑127 (MQ=255)                          cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGt                                                                                                                    <  2:861375/127‑1 (MQ=255)                          cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCa                                                                                                        >  1:841425/1‑139 (MQ=255)                                     cGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCa                                                                                             <  2:82496/139‑1 (MQ=255)                                      ggATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCAt                                                                                            <  1:1272314/139‑1 (MQ=255)                                       gATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATc                                                                                           >  1:103848/1‑139 (MQ=255)                                       gATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATc                                                                                           <  2:103848/139‑1 (MQ=255)                                              cGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAg                                                                                    >  1:514997/1‑139 (MQ=255)                                               gTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGa                                                                                   >  2:1261553/1‑139 (MQ=255)                                                 tGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATg                                                                                 <  1:1261553/139‑1 (MQ=255)                                                 tGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATg                                                                                 >  1:615182/1‑139 (MQ=255)                                                  gAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGc                                                                                <  1:116071/139‑1 (MQ=255)                                                   aaTCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCa                                                                               <  2:841425/139‑1 (MQ=255)                                                      cagcagCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATc                                                                            >  2:597383/1‑139 (MQ=255)                                                        gcagcaTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCca                                                                          <  1:975087/139‑1 (MQ=255)                                                              tatGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACAcccc                                                                    >  1:11121/1‑139 (MQ=255)                                                                tgctgcCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAg                                                                  <  2:11121/139‑1 (MQ=255)                                                                tgctgcCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAg                                                                  <  1:466387/139‑1 (MQ=255)                                                                      cGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGAc                                                                                                              <  1:494811/89‑1 (MQ=255)                                                                      cGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGAc                                                                                                              >  2:494811/1‑89 (MQ=255)                                                                      cGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCa                                                                               <  2:1408226/120‑1 (MQ=255)                                                                      cGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCa                                                                               >  1:1408226/1‑120 (MQ=255)                                                                                aaaCGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTctgctg                                                  <  1:239700/139‑1 (MQ=255)                                                                                     gCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATc                                                                                           >  1:122957/1‑93 (MQ=255)                                                                                     gCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATc                                                                                           <  2:122957/93‑1 (MQ=255)                                                                                     gCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcgcgc                                             >  2:955294/1‑139 (MQ=255)                                                                                                  tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                <  1:955294/139‑1 (MQ=255)                                                                                                  tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                <  2:615182/139‑1 (MQ=255)                                                                                                     gCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                >  1:949466/1‑136 (MQ=255)                                                                                                     gCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                <  2:949466/136‑1 (MQ=255)                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCaa                                                                                                  <  1:1111821/69‑1 (MQ=255)                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCaa                                                                                                  >  2:1111821/1‑69 (MQ=255)                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGt                            >  1:815523/1‑139 (MQ=255)                                                                                                          gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTggg                                                                                                                             >  1:319891/1‑38 (MQ=255)                                                                                                          gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTggg                                                                                                                             <  2:319891/38‑1 (MQ=255)                                                                                                          gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCa                                                                                                            >  2:1264132/1‑55 (MQ=255)                                                                                                          gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCa                                                                                                            <  1:1264132/55‑1 (MQ=255)                                                                                                                cgcAGCCTGACATTCCGTTGCAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATCGACGGTATAAACCAGa                  >  1:970043/1‑139 (MQ=255)                                                                                                                cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGTTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCCCCCTGGGGTTTATTACCGGTATAAACCAGa                  >  2:998174/1‑139 (MQ=255)                                                                                                                cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGt                            >  1:73439/1‑129 (MQ=255)                                                                                                                cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGt                            <  2:73439/129‑1 (MQ=255)                                                                                                                             tCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGAc                               <  2:182629/113‑1 (MQ=255)                                                                                                                             tCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGAc                               >  1:182629/1‑113 (MQ=255)                                                                                                                              ccGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGCATAAACCAGAATCCGCAGTCCGGc    <  2:970043/139‑1 (MQ=255)                                                                                                                               cGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAgcgc                                                               <  2:1909/79‑1 (MQ=255)                                                                                                                               cGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAgcgc                                                               >  1:1909/1‑79 (MQ=255)                                                                                                                                gTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAAGCCGCAGTCCGGCGt  <  1:1217712/139‑1 (MQ=255)                                                                                                                                    aaaaTTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCt                                            >  2:1172803/1‑93 (MQ=255)                                                                                                                                    aaaaTTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCt                                            <  1:1172803/93‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   ACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGT  >  NC_000913/3587235‑3587500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑