Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,589,820 T→C S70G (AGC→GGC)  ugpE ← sn‑glycerol‑3‑phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,589,8200TC100.0% 290.0 / NA 79S70G (AGC→GGC) ugpEsn‑glycerol‑3‑phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/41);  total (38/41)

AGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGA  >  NC_000913/3589685‑3589954
                                                                                                                                       |                                                                                                                                      
agaagaagaGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                     <  2:1153917/139‑1 (MQ=255)
agaagaagaGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                     <  2:616934/139‑1 (MQ=255)
 gaagaagaGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTc                                                                                                                                    <  1:1353735/139‑1 (MQ=255)
   agaagaGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGt                                                                                                                                  <  1:389191/139‑1 (MQ=255)
            tACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAcccc                                                                                                                         >  2:1271736/1‑139 (MQ=255)
            tACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAcccc                                                                                                                         >  1:1287250/1‑139 (MQ=255)
             aCGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCg                                                                                                                        <  1:664635/139‑1 (MQ=255)
              cGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                       <  1:746808/139‑1 (MQ=255)
              cGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAAAGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                       >  2:1339280/1‑139 (MQ=255)
                   cGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                      >  2:549301/1‑135 (MQ=255)
                   cGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                      <  1:549301/135‑1 (MQ=255)
                   cGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCAcc                                                                                                                  >  2:1274257/1‑139 (MQ=255)
                       aaaCGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGa                                                                                                              <  2:573181/139‑1 (MQ=255)
                             aaCCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGAtgttgt                                                                                                        >  2:486828/1‑139 (MQ=255)
                                cAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCTTACCCCGTTCACCCAGATGTTGTGGa                                                                                                     <  1:211929/139‑1 (MQ=255)
                                cAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGa                                                                                                     <  1:39840/139‑1 (MQ=255)
                                     aaTGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGttt                                                                                                <  1:486828/139‑1 (MQ=255)
                                     aaTGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGttt                                                                                                <  1:234758/139‑1 (MQ=255)
                                       tGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCg                                                                                                                        >  1:481184/1‑113 (MQ=255)
                                       tGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCg                                                                                                                        <  2:481184/113‑1 (MQ=255)
                                       tGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTc                                                                                              <  1:269351/139‑1 (MQ=255)
                                       tGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTc                                                                                              >  1:691875/1‑139 (MQ=255)
                                                ccGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAtg                                                                                     <  1:1274257/139‑1 (MQ=255)
                                                 cGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATTCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                       <  1:891690/104‑1 (MQ=255)
                                                 cGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                       >  2:891690/1‑104 (MQ=255)
                                                  gagaGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAtgtg                                                                                   <  1:1271736/139‑1 (MQ=255)
                                                  gagaGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAtgtg                                                                                   <  2:86878/139‑1 (MQ=255)
                                                   agagCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCAccc                                                                                                                 <  2:760784/108‑1 (MQ=255)
                                                   agagCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCAccc                                                                                                                 >  1:760784/1‑108 (MQ=255)
                                                       cATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCg                                                                              >  2:612461/1‑139 (MQ=255)
                                                        aTCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCgg                                                                             >  2:69324/1‑139 (MQ=255)
                                                         tCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAt                                                                                      <  2:55590/129‑1 (MQ=255)
                                                         tCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAt                                                                                      >  1:55590/1‑129 (MQ=255)
                                                         tCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCggg                                                                            >  2:194926/1‑139 (MQ=255)
                                                         tCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCggg                                                                            >  1:811756/1‑139 (MQ=255)
                                                         tCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCggg                                                                            >  2:1206570/1‑139 (MQ=255)
                                                          cGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCgggg                                                                           <  1:577567/139‑1 (MQ=255)
                                                               cGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGa                                                                                                     >  2:699336/1‑108 (MQ=255)
                                                               cGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGa                                                                                                     <  1:699336/108‑1 (MQ=255)
                                                                 gTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCa                                                                                                                    >  2:290542/1‑91 (MQ=255)
                                                                 gTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCa                                                                                                                    <  1:290542/91‑1 (MQ=255)
                                                                 gTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCg                                                                    >  2:1147833/1‑139 (MQ=255)
                                                                        tGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCgg                                                             <  1:69324/139‑1 (MQ=255)
                                                                         gCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGTCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgc                                                            <  1:1158782/139‑1 (MQ=255)
                                                                         gCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGa                                                                                                              >  1:376738/1‑89 (MQ=255)
                                                                         gCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGa                                                                                                              <  2:376738/89‑1 (MQ=255)
                                                                                gTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGACGCGGCAt                                                     >  2:120131/1‑139 (MQ=255)
                                                                                   aTGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGa                                                  <  1:1339280/139‑1 (MQ=255)
                                                                                     gCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGACGCGGCATAGACg                                                <  1:120131/139‑1 (MQ=255)
                                                                                        gAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGcc                                             >  1:482231/1‑139 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGTACGGCGCGCCGTTCGTGCCTACCCCGATCACCCAGATGTTGTGGATGTTTGTCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCt                                            >  1:908767/1‑139 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAc                                                                                                                            <  2:1289582/59‑1 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAc                                                                                                                            >  1:1289582/1‑59 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                       >  1:227142/1‑64 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                       <  2:227142/64‑1 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGATGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCt                                            >  2:201137/1‑139 (MQ=255)
                                                                                          aCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                      >  2:1390375/1‑64 (MQ=255)
                                                                                          aCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                      <  1:1390375/64‑1 (MQ=255)
                                                                                           cGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGt                                          >  1:612829/1‑139 (MQ=255)
                                                                                              cATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACgg                                               >  1:1275150/1‑131 (MQ=255)
                                                                                              cATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACgg                                               <  2:1275150/131‑1 (MQ=255)
                                                                                                   caAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAg                                  >  2:741338/1‑139 (MQ=255)
                                                                                                        cTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTcg                             <  2:811756/139‑1 (MQ=255)
                                                                                                          gTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTcgcc                           >  2:1331541/1‑139 (MQ=255)
                                                                                                           ttAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTcgccg                          >  1:120200/1‑139 (MQ=255)
                                                                                                            tAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGAtg                                                                                                            >  2:284695/1‑56 (MQ=255)
                                                                                                            tAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGAtg                                                                                                            <  1:284695/56‑1 (MQ=255)
                                                                                                               gCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGAc                      <  1:741338/139‑1 (MQ=255)
                                                                                                                     tCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGc                >  2:150648/1‑139 (MQ=255)
                                                                                                                            gAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTAc         <  1:333209/139‑1 (MQ=255)
                                                                                                                             aaCGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACa        >  1:720769/1‑139 (MQ=255)
                                                                                                                               cGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGc      <  1:1206570/139‑1 (MQ=255)
                                                                                                                               cGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTGGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGc      <  2:691875/139‑1 (MQ=255)
                                                                                                                                ggCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCg     <  2:1287250/139‑1 (MQ=255)
                                                                                                                                 gcgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCgg    >  1:754800/1‑139 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgc                                                            >  1:1326802/1‑82 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgc                                                            <  2:1326802/82‑1 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCggg   <  2:754800/139‑1 (MQ=255)
                                                                                                                                   gcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGa  >  2:646885/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
AGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGA  >  NC_000913/3589685‑3589954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: