Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,694,840	G→A	H133Y (CAT→TAT)	bcsA ←	cellulose synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,694,840	0	G	A	100.0%	163.8 / NA	51	H133Y (CAT→TAT)	bcsA	cellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/27);  total (24/27)

AAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATA  >  NC_000913/3694706‑3694971                                                                                                                                       |                                                                                                                                    aaaCTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACtgt                                                                                                                                 <  2:291155/139‑1 (MQ=255)      gcgcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGtttt                                                                                                                            >  2:331405/1‑139 (MQ=255)      gcgcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGtttt                                                                                                                            >  1:438935/1‑139 (MQ=255)         ccAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATg                                                                                                                         >  1:68551/1‑139 (MQ=255)            gCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCt                                                                                                                      >  1:344414/1‑139 (MQ=255)                               aaCGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCg                                                                                                   <  2:1345980/139‑1 (MQ=255)                                 cGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGtt                                                                                                 <  1:331405/139‑1 (MQ=255)                                      aTTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    <  2:359774/115‑1 (MQ=255)                                      aTTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                    >  1:359774/1‑115 (MQ=255)                                        tAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCAt                                                                                          <  2:68551/139‑1 (MQ=255)                                         aaCGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCAtt                                                                                         <  1:988314/139‑1 (MQ=255)                                            gCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCa                                                                                               >  1:210882/1‑130 (MQ=255)                                            gCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCa                                                                                               <  2:210882/130‑1 (MQ=255)                                                gAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTa                                                                                  >  2:946081/1‑139 (MQ=255)                                                          gaAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAg                                                                                                       >  1:1375897/1‑108 (MQ=255)                                                          gaAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAg                                                                                                       <  2:1375897/108‑1 (MQ=255)                                                           aaGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTGACGTCCACGa                                                                        >  1:930904/1‑138 (MQ=255)                                                           aaGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGa                                                                        <  2:930904/138‑1 (MQ=255)                                                                gACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                              <  2:53826/111‑1 (MQ=255)                                                                gACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                              >  1:53826/1‑111 (MQ=255)                                                                 aCGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATAtttt                                                                 >  2:1402078/1‑139 (MQ=255)                                                                  cGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTg                                                                <  2:438935/139‑1 (MQ=255)                                                                            gAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGaa                                                      <  1:1402078/139‑1 (MQ=255)                                                                                          gcgcCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGttt                                                                                                                             >  1:117099/1‑54 (MQ=255)                                                                                          gcgcCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGttt                                                                                                                             <  2:117099/54‑1 (MQ=255)                                                                                           cgcCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTgcgc                                       >  2:476184/1‑139 (MQ=255)                                                                                                cGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGttt                                  <  1:476184/139‑1 (MQ=255)                                                                                                     cTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGtt                                                                                                 <  2:911106/71‑1 (MQ=255)                                                                                                     cTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGtt                                                                                                 >  1:911106/1‑71 (MQ=255)                                                                                                        aagtGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCAc                                                                          <  2:651257/88‑1 (MQ=255)                                                                                                        aagtGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCAc                                                                          >  1:651257/4‑91 (MQ=255)                                                                                                        aaaTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAg                                                                                                       <  2:573376/62‑1 (MQ=255)                                                                                                        aaaTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAg                                                                                                       >  1:573376/1‑62 (MQ=255)                                                                                                          aTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCa                                                                                                        <  1:331344/59‑1 (MQ=255)                                                                                                          aTGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCa                                                                                                        >  2:331344/1‑59 (MQ=255)                                                                                                            gACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCttt                      <  2:1414626/139‑1 (MQ=255)                                                                                                               cGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTgcg                   <  2:344414/139‑1 (MQ=255)                                                                                                                 aGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGcgcg                 <  1:594144/139‑1 (MQ=255)                                                                                                                  gCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCg                                                                                                   >  1:730137/1‑56 (MQ=255)                                                                                                                  gCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCg                                                                                                   <  2:730137/56‑1 (MQ=255)                                                                                                                  gCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTgcg                   >  2:329827/1‑136 (MQ=255)                                                                                                                  gCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTgcg                   <  1:329827/136‑1 (MQ=255)                                                                                                                  gCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGa                >  2:1324555/1‑139 (MQ=255)                                                                                                                       tttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTCCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAc           <  1:946081/139‑1 (MQ=255)                                                                                                                        ttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          <  1:1324555/139‑1 (MQ=255)                                                                                                                             tcATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCa     >  2:1327073/1‑139 (MQ=255)                                                                                                                                tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTgcg                                        <  2:25603/101‑1 (MQ=255)                                                                                                                                tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTgcg                                        >  1:25603/1‑101 (MQ=255)                                                                                                                                tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          >  1:672588/1‑131 (MQ=255)                                                                                                                                tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc          <  2:672588/131‑1 (MQ=255)                                                                                                                                tCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATa  >  1:172010/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    AAACTGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCAATA  >  NC_000913/3694706‑3694971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑