Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,695,997	C→T	*63* (TAG→TAA)	yhjR ←	DUF2629 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,695,997	0	C	T	100.0%	182.5 / NA	58	*63* (TAG→TAA)	yhjR	DUF2629 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/27);  total (31/27)

TGTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGG  >  NC_000913/3695862‑3696132                                                                                                                                        |                                                                                                                                        tGTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTAc                                                                                                                                      >  2:1295113/1‑139 (MQ=255)          aggcaTCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgc                                                                                                                             >  2:1046035/1‑139 (MQ=255)                  accaccAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcgc                                                                                                                           >  1:471982/1‑133 (MQ=255)                  accaccAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcgc                                                                                                                           <  2:471982/133‑1 (MQ=255)                                ttCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGcc                                                                                                       <  2:232254/139‑1 (MQ=255)                                ttCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGcc                                                                                                       <  1:1046035/139‑1 (MQ=255)                                 tctcCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaaa                                                                                                                        >  1:1140233/1‑121 (MQ=255)                                 tctcCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaaa                                                                                                                        <  2:1140233/121‑1 (MQ=255)                                 tctcCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCa                                                                                                      >  1:364041/1‑139 (MQ=255)                                                              tGCGGCGGTGATGGTTGTTGTCCCCGCGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgc                                                                         >  1:803743/1‑139 (MQ=255)                                                              tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGtt                                                                            <  2:466089/136‑1 (MQ=255)                                                              tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGtt                                                                            >  1:466089/1‑136 (MQ=255)                                                              tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgc                                                                         >  2:47539/1‑139 (MQ=255)                                                              tGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgc                                                                         >  2:359982/1‑139 (MQ=255)                                                               gcggcgGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgcg                                                                        <  1:359982/139‑1 (MQ=255)                                                                   cggTGATGGTTGTTGTGCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTCGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   >  1:1384236/1‑124 (MQ=255)                                                                   cggTGATGGTTGTTGTCCCCACGCCTCCCCGCTCCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTgg                                                                    >  2:850315/1‑139 (MQ=255)                                                                   cggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAGCTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   <  2:1384236/124‑1 (MQ=255)                                                                              gttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAATTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGc                                                         <  1:850315/139‑1 (MQ=255)                                                                               ttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                        <  2:364041/139‑1 (MQ=255)                                                                               ttgtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCa                                                        <  1:121185/139‑1 (MQ=255)                                                                                 gtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAAT‑CCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGa                                                                  <  1:643005/126‑1 (MQ=255)                                                                                 gtCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAAT‑CCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGa                                                                  >  2:643005/1‑126 (MQ=255)                                                                                    cccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                        >  1:181339/1‑86 (MQ=255)                                                                                    cccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGc                                                                                                        <  2:181339/86‑1 (MQ=255)                                                                                     ccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACg                                                                                                          >  1:585631/1‑83 (MQ=255)                                                                                     ccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACg                                                                                                          <  2:585631/83‑1 (MQ=255)                                                                                      cACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaa                                                                                                                         >  2:1044471/1‑67 (MQ=255)                                                                                      cACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCaa                                                                                                                         <  1:1044471/67‑1 (MQ=255)                                                                                      cACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCaa                                                 >  1:530021/1‑139 (MQ=255)                                                                                         gCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCATTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAatat                                              <  2:803743/139‑1 (MQ=255)                                                                                           cTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAATGGc                                                                                                        >  1:1387801/1‑79 (MQ=255)                                                                                           cTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAATGGc                                                                                                        <  2:1387801/79‑1 (MQ=255)                                                                                           cTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAg                                                                                                     >  1:1330824/1‑82 (MQ=255)                                                                                           cTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAg                                                                                                     <  2:1330824/82‑1 (MQ=255)                                                                                                  cACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgcg                                                                        <  2:428339/104‑1 (MQ=255)                                                                                                  cACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTcgcg                                                                        >  1:428339/1‑104 (MQ=255)                                                                                                       ccTGCTATCCCCGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACACTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCa                                        <  1:1105292/131‑1 (MQ=255)                                                                                                       ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                            >  1:771412/1‑47 (MQ=255)                                                                                                       ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTgcg                                                                                                                            <  2:771412/47‑1 (MQ=255)                                                                                                       ccTGCTATCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCa                                        >  2:1105292/1‑131 (MQ=255)                                                                                                             aTCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATg                                                                                           >  1:10229/1‑74 (MQ=255)                                                                                                             aTCCCAGTACGGCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATg                                                                                           <  2:10229/74‑1 (MQ=255)                                                                                                                       ggCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca                <  1:47539/139‑1 (MQ=255)                                                                                                                       ggCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca                >  1:50940/1‑139 (MQ=255)                                                                                                                        gCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   >  1:1217166/1‑71 (MQ=255)                                                                                                                        gCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGcc                                                                                   <  2:1217166/71‑1 (MQ=255)                                                                                                                        gCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTgg                                                                    <  2:672777/86‑1 (MQ=255)                                                                                                                        gCCATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTgg                                                                    >  1:672777/1‑86 (MQ=255)                                                                                                                         ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTggg                                                                   >  1:1107246/1‑86 (MQ=255)                                                                                                                         ccATTCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTggg                                                                   <  2:1107246/86‑1 (MQ=255)                                                                                                                            ttCACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAata           >  2:1226202/1‑139 (MQ=255)                                                                                                                              cACCAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATc         >  2:717092/1‑139 (MQ=255)                                                                                                                                 cAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGc                                         >  2:1369620/1‑104 (MQ=255)                                                                                                                                 cAATCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGc                                         <  1:1369620/104‑1 (MQ=255)                                                                                                                                   aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCt    >  2:1331442/1‑139 (MQ=255)                                                                                                                                   aTCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGAGAAAATGCCTGCTTTAACGCCACAATATCATTCt    >  2:1232159/1‑139 (MQ=255)                                                                                                                                    tCCCTTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTg   <  2:50940/139‑1 (MQ=255)                                                                                                                                     ccctTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg  <  1:1232159/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGTCCGGGCAGGCATCGACCACCAGGACATTTTCTCCCAACATTTGTAATGACCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGG  >  NC_000913/3695862‑3696132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑