Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	345,649	G→A	*82* (TAG→TAA)	yahM →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	345,649	0	G	A	100.0%	159.1 / NA	50	*82* (TAG→TAA)	yahM	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

AAAAAGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAGAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACAGCGGGCA  >  NC_000913/345513‑345781                                                                                                                                         |                                                                                                                                     aaaaaGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTaaag                                                                                                                                    <  2:1412406/139‑1 (MQ=255) aaaaaGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTaaag                                                                                                                                    <  2:1274175/139‑1 (MQ=255)   aaaGCCCACACAGGGGAGAGTGGGCTGAAATGGGAGGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTaaagag                                                                                                                                  >  2:221093/1‑139 (MQ=255)    aaGCCCACACAGGGGAGAGTGGGCTGAAATGGGAGGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGt                                                                                                                                 <  1:221093/139‑1 (MQ=255)                     agTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTATATTATCTGCTCACCg                                                                                                                >  1:1397148/1‑139 (MQ=255)                          gCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCAc                                                                                                                  >  1:542954/1‑132 (MQ=255)                          gCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCAc                                                                                                                  <  2:542954/132‑1 (MQ=255)                            tGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTc                                                                                                         >  2:1099730/1‑139 (MQ=255)                            tGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTc                                                                                                         >  2:481887/1‑139 (MQ=255)                                   ggAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCtt                                                                                                  >  1:1053249/1‑139 (MQ=255)                                     aaGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCg                                                                                                <  2:1053249/139‑1 (MQ=255)                                               atCAAGTAACCTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCGGCGGCGATGGCTGTGATTCGGAAAATCAGGTTTATTACGTAAAGTGTAAAGTATTTGCTCACCGCTGCGGCACCCCTTCGTAAATCAGGc                                                                                      <  2:270151/138‑1 (MQ=255)                                                tCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCg                                                                                     >  1:446598/1‑139 (MQ=255)                                                 cAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGt                                                                                    <  1:1099730/139‑1 (MQ=255)                                                          ttATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAAt                                                                           >  2:634528/1‑139 (MQ=255)                                                           tATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATa                                                                          <  1:259052/139‑1 (MQ=255)                                                            aTCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGaa                                                                            >  1:74545/1‑136 (MQ=255)                                                            aTCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGaa                                                                            <  2:74545/136‑1 (MQ=255)                                                             tCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTg                                                                                                                       <  2:197571/92‑1 (MQ=255)                                                             tCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTg                                                                                                                       >  1:197571/1‑92 (MQ=255)                                                              cGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATacac                                                                       >  1:1265041/1‑139 (MQ=255)                                                                  aaTAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCg                                                                                                                >  2:143493/1‑94 (MQ=255)                                                                  aaTAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCg                                                                                                                <  1:143493/94‑1 (MQ=255)                                                                       ggACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTg                                                              <  1:1156496/139‑1 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                          <  2:919781/75‑1 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                          >  1:919781/1‑75 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                          <  2:1352954/75‑1 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                          >  1:1352954/1‑75 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTg                                                              <  2:1006772/135‑1 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCATAAATCAGGCGTAGCGCGAATACACCAATAATTg                                                              >  1:1006772/1‑135 (MQ=255)                                                                             cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                          <  2:330348/73‑1 (MQ=255)                                                                             cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                          >  1:330348/1‑73 (MQ=255)                                                                             cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGt                                                                                               >  1:911892/1‑100 (MQ=255)                                                                             cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGt                                                                                               <  2:911892/100‑1 (MQ=255)                                                                                  tACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCt                                                                                                                     <  1:92527/73‑1 (MQ=255)                                                                                  tACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCt                                                                                                                     >  2:92527/1‑73 (MQ=255)                                                                                        ggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                                     >  2:948253/1‑83 (MQ=255)                                                                                        ggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                                     <  1:948253/83‑1 (MQ=255)                                                                                          cTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                                     <  1:803946/81‑1 (MQ=255)                                                                                          cTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                                     >  2:803946/1‑81 (MQ=255)                                                                                            atcatcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCaa                                         >  1:504916/1‑139 (MQ=255)                                                                                              catcGCCGATGGCCGTGATTCGGCAAATCAGGTTGACTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAAt                                                                                           <  1:920048/87‑1 (MQ=255)                                                                                              catcGCCGATGGCCGTGATTCGGCAAATCAGGTTGACTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAAt                                                                                           >  2:920048/1‑87 (MQ=255)                                                                                                tcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAAcgcg                                     <  1:481887/139‑1 (MQ=255)                                                                                                   ccGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                                  <  2:684387/91‑1 (MQ=255)                                                                                                   ccGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                                  >  1:684387/1‑91 (MQ=255)                                                                                                           cGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGt                                                                                    >  2:702137/1‑81 (MQ=255)                                                                                                           cGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGt                                                                                    <  1:702137/81‑1 (MQ=255)                                                                                                                   ggCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACAcc                                                                      >  1:783961/1‑87 (MQ=255)                                                                                                                   ggCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACAcc                                                                      <  2:783961/87‑1 (MQ=255)                                                                                                                        aTCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATa                              >  2:1018278/1‑122 (MQ=255)                                                                                                                        aTCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATa                              <  1:1018278/122‑1 (MQ=255)                                                                                                                                   tACGTAAAGAGTATATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACAGCGGGCa  <  2:1397148/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     AAAAAGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAGAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACAGCGGGCA  >  NC_000913/345513‑345781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑