Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,766,316	G→A	*1378* (TAG→TAA)	rhsA →	Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,766,316	0	G	A	100.0%	274.2 / NA	84	*1378* (TAG→TAA)	rhsA	Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/50);  total (34/50)

ATGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCT  >  NC_000913/3766180‑3766427                                                                                                                                           |                                                                                                                aTGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAAcc                                                                                                               >  2:1409149/1‑139 (MQ=255) aTGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTC‑TCACTCCAGACAGTCAAACGATAAAAAAAGATAAccc                                                                                                              <  2:999704/139‑1 (MQ=255)   gggAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCt                                                                                                             <  2:1383848/139‑1 (MQ=255)         aaaCCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                                       <  2:1206918/139‑1 (MQ=255)           aCCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                                       <  2:1210377/139‑1 (MQ=255)                   gATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             <  1:420636/139‑1 (MQ=255)                   gATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             <  1:1219650/139‑1 (MQ=255)                   gATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             <  2:432898/139‑1 (MQ=255)                   gATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             <  1:1409149/139‑1 (MQ=255)                   gATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             <  2:192860/139‑1 (MQ=255)                   gATGTGACGTACGTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             <  2:658904/139‑1 (MQ=255)                      gtgACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             <  1:172577/136‑1 (MQ=255)                      gtgACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             >  2:172577/1‑136 (MQ=255)                               ttCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTc                                                                                        >  1:81524/1‑132 (MQ=255)                               ttCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTc                                                                                        <  2:81524/132‑1 (MQ=255)                                        tAATTGATTGGGGTGCTATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaa                                                                        >  2:954113/1‑139 (MQ=255)                                         aaTTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaa                                                                       >  1:117178/1‑139 (MQ=255)                                         aaTTGATTGTGGTGATATCA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGc                                                                                              <  1:159152/116‑1 (MQ=255)                                         aaTTGATTGTGGTGATATCA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGc                                                                                              >  2:159152/1‑116 (MQ=255)                                           ttgattgTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaaga                                                                     <  2:117178/139‑1 (MQ=255)                                           ttgattgTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaaga                                                                     <  1:578382/139‑1 (MQ=255)                                               ttgTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAgg                                                                 <  1:1319054/139‑1 (MQ=255)                                                tgtgGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGc                                                                >  2:1022423/1‑139 (MQ=255)                                                  tggtgATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCaa                                                              >  2:311477/1‑139 (MQ=255)                                                    gtgATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGt                                                            >  2:629401/1‑139 (MQ=255)                                                    gtgATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGt                                                            >  2:462247/1‑139 (MQ=255)                                                     tgATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           <  2:838554/139‑1 (MQ=255)                                                     tgATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           <  1:462247/139‑1 (MQ=255)                                                     tgATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           <  1:954113/139‑1 (MQ=255)                                                     tgATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           <  1:629401/139‑1 (MQ=255)                                                     tgATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           <  1:90534/139‑1 (MQ=255)                                                           tA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                     <  1:1022423/139‑1 (MQ=255)                                                           tA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                     <  2:1038326/139‑1 (MQ=255)                                                           tA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                     <  1:311477/139‑1 (MQ=255)                                                                tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                     >  1:751128/1‑136 (MQ=255)                                                                tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                     <  2:751128/136‑1 (MQ=255)                                                                  cTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGt                                                >  2:1174403/1‑139 (MQ=255)                                                                  cTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTAAGGt                                                >  2:965695/1‑139 (MQ=255)                                                                    aaaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCaaa                                                                                     >  1:1289352/1‑100 (MQ=255)                                                                    aaaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCaaa                                                                                     <  2:1289352/100‑1 (MQ=255)                                                                    aaaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCaa                                                              >  1:1257689/1‑123 (MQ=255)                                                                    aaaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCaa                                                              <  2:1257689/123‑1 (MQ=255)                                                                     aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAatacatac                                                                              <  1:805581/106‑1 (MQ=255)                                                                     aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAatacatac                                                                              >  2:805581/1‑106 (MQ=255)                                                                     aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           >  2:792132/1‑125 (MQ=255)                                                                     aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           <  1:792132/125‑1 (MQ=255)                                                                      aGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCaaa                                                                                     >  2:126038/1‑98 (MQ=255)                                                                      aGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCaaa                                                                                     <  1:126038/98‑1 (MQ=255)                                                                         tGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGt                                                                                                    <  2:27494/80‑1 (MQ=255)                                                                         tGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGt                                                                                                    >  1:27494/1‑80 (MQ=255)                                                                         tGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTtatata                                         <  1:1174403/139‑1 (MQ=255)                                                                         tGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTtatata                                         <  1:965695/139‑1 (MQ=255)                                                                          gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                     <  2:299612/126‑1 (MQ=255)                                                                          gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                     >  1:299612/1‑126 (MQ=255)                                                                           cAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAataca                                                                                 >  2:754588/1‑97 (MQ=255)                                                                           cAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAataca                                                                                 <  1:754588/97‑1 (MQ=255)                                                                                 gCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             <  1:384425/79‑1 (MQ=255)                                                                                 gCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                                             >  2:384425/1‑79 (MQ=255)                                                                                    cacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAatacatac                                                                              <  2:1149635/91‑1 (MQ=255)                                                                                    cacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACACTTGTGGAGGTTCCTGCAATGTCAAatacatac                                                                              >  1:1149635/1‑91 (MQ=255)                                                                                       aGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                     <  1:946336/113‑1 (MQ=255)                                                                                       aGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                     >  2:946336/1‑113 (MQ=255)                                                                                         aaaGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaaga                                                                     <  1:103819/95‑1 (MQ=255)                                                                                         aaaGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaaga                                                                     >  2:103819/1‑95 (MQ=255)                                                                                          aaGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTtata                                           <  1:488936/120‑1 (MQ=255)                                                                                          aaGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTtata                                           >  2:488936/1‑120 (MQ=255)                                                                                               tGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           >  2:47999/1‑99 (MQ=255)                                                                                               tGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           <  1:47999/99‑1 (MQ=255)                                                                                                       tcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTtgtg                                                                                                         <  1:623436/45‑1 (MQ=255)                                                                                                       tcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTtgtg                                                                                                         >  2:623436/1‑45 (MQ=255)                                                                                                       tcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           >  1:1189036/1‑91 (MQ=255)                                                                                                       tcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                           <  2:1189036/91‑1 (MQ=255)                                                                                                              tCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                                       >  1:1113597/1‑40 (MQ=255)                                                                                                              tCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                                       <  2:1113597/40‑1 (MQ=255)                                                                                                              tCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCg    <  1:226009/139‑1 (MQ=255)                                                                                                                cAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCt  <  2:164983/139‑1 (MQ=255)                                                                                                                       tCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCCAGTAAAGAGTa                                                    <  1:31598/82‑1 (MQ=255)                                                                                                                       tCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCCAGTAAAGAGTa                                                    >  2:31598/1‑82 (MQ=255)                                                                                                                       tCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGaa            <  1:1324392/122‑1 (MQ=255)                                                                                                                       tCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGaa            >  2:1324392/1‑122 (MQ=255)                                                                                                                        cAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACt                         <  2:1139558/108‑1 (MQ=255)                                                                                                                        cAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACt                         >  1:1139558/1‑108 (MQ=255)                                                                                                                          aaCGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAatgatg                 >  1:389905/1‑114 (MQ=255)                                                                                                                          aaCGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAatgatg                 <  2:389905/114‑1 (MQ=255)                                                                                                                                           |                                                                                                                ATGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTA‑‑GTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCT  >  NC_000913/3766180‑3766427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑