Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,804,068	T→C	I24V (ATT→GTT)	waaB ←	lipopolysaccharide 1,6‑galactosyltransferase, UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,804,068	0	T	C	100.0%	226.5 / NA	62	I24V (ATT→GTT)	waaB	lipopolysaccharide 1,6‑galactosyltransferase, UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/31);  total (31/31)

ACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGA  >  NC_000913/3803934‑3804201                                                                                                                                       |                                                                                                                                      aCGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAacaaca                                                                                                                                   >  2:1101333/1‑139 (MQ=255) aCGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAacaaca                                                                                                                                   >  1:751699/1‑139 (MQ=255)         aaCCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGAt                                                                                                                           <  2:63224/139‑1 (MQ=255)          aCCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATg                                                                                                                          <  1:1101333/139‑1 (MQ=255)          aCCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCA‑CCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGa                                                                                                                         <  2:463139/139‑1 (MQ=255)                     tatTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                               >  2:812534/1‑139 (MQ=255)                                              aaTACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCg                                                                                      <  2:1050253/139‑1 (MQ=255)                                                 aCCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCa                                                                                                                                >  1:437858/1‑94 (MQ=255)                                                 aCCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCa                                                                                                                                <  2:437858/94‑1 (MQ=255)                                                 aCCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCg                                                                                   >  1:76910/1‑139 (MQ=255)                                                   cTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGAt                                                                                 >  2:304432/1‑139 (MQ=255)                                                     tCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaa                                                                               >  1:343897/1‑139 (MQ=255)                                                       cAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                               <  1:585851/105‑1 (MQ=255)                                                       cAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                               >  2:585851/1‑105 (MQ=255)                                                       cAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAAc                                                                             <  2:578943/139‑1 (MQ=255)                                                        aGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACg                                                                            <  1:812534/139‑1 (MQ=255)                                                         gCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGc                                                                           <  2:343897/139‑1 (MQ=255)                                                         gCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGc                                                                           >  1:701140/1‑139 (MQ=255)                                                          ccATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCtt                                                                                        <  1:236136/125‑1 (MQ=255)                                                          ccATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCtt                                                                                        >  2:236136/1‑125 (MQ=255)                                                          ccATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCt                                                                          >  2:98519/1‑139 (MQ=255)                                                                   aTCCATTTTGTCATTACGACAAAAGAAAAACATTTCGCTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCACCGATAAACGCTATTTTCATa                                                                 >  1:839785/1‑139 (MQ=255)                                                                   aTCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATa                                                                 <  2:1342162/139‑1 (MQ=255)                                                                    tCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAg                                                                >  2:1354388/1‑139 (MQ=255)                                                                     ccATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGa                                                               <  1:1354388/139‑1 (MQ=255)                                                                       aTTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                             <  1:34386/139‑1 (MQ=255)                                                                       aTTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                             <  2:751699/139‑1 (MQ=255)                                                                        ttttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAAc                                                            >  2:1210525/1‑139 (MQ=255)                                                                         tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                        >  2:476305/1‑94 (MQ=255)                                                                         tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                        <  1:476305/94‑1 (MQ=255)                                                                         tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCTCCGATAAACGCTATTTTCATAGAAAct                                                           >  1:1131779/1‑139 (MQ=255)                                                                           tGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGa                                                                                                                            >  1:1026013/1‑72 (MQ=255)                                                                           tGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGa                                                                                                                            <  2:1026013/72‑1 (MQ=255)                                                                              cATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCAt                                                      >  1:263538/1‑139 (MQ=255)                                                                               aTTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                             <  2:1092316/131‑1 (MQ=255)                                                                               aTTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                             >  1:1092316/1‑131 (MQ=255)                                                                               aTTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCAtt                                                     >  1:749532/1‑139 (MQ=255)                                                                               aTTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCAtt                                                     >  1:531827/1‑139 (MQ=255)                                                                                     aCAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGa                                               >  2:671/1‑139 (MQ=255)                                                                                       aaaaGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGt                                             <  2:1288083/139‑1 (MQ=255)                                                                                              aaaCATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTc                                      <  1:304432/139‑1 (MQ=255)                                                                                                aCATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                                    <  2:749532/139‑1 (MQ=255)                                                                                                aCATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                                    <  1:672435/139‑1 (MQ=255)                                                                                                   tttCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTaa                                                   >  2:10956/1‑121 (MQ=255)                                                                                                   tttCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTaa                                                   <  1:10956/121‑1 (MQ=255)                                                                                                       gcTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATCGAGTAAAACTCACTGTTTTa                             <  2:839785/138‑1 (MQ=255)                                                                                                        cTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTaa                            <  2:531827/139‑1 (MQ=255)                                                                                                                  gttgttGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCGGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTAtt                  >  2:486607/1‑139 (MQ=255)                                                                                                                   ttgttgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTAttt                 <  2:263538/139‑1 (MQ=255)                                                                                                                    tgttgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTg                <  2:701140/139‑1 (MQ=255)                                                                                                                       tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                        >  1:720872/1‑48 (MQ=255)                                                                                                                       tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                        <  2:720872/48‑1 (MQ=255)                                                                                                                       tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAAc                           >  1:1009448/1‑125 (MQ=255)                                                                                                                       tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCt             >  1:432813/1‑139 (MQ=255)                                                                                                                       tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATCTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAAc                           <  2:1009448/125‑1 (MQ=255)                                                                                                                       tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCACCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGt               >  1:1324407/1‑137 (MQ=255)                                                                                                                       tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCACCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGt               <  2:1324407/137‑1 (MQ=255)                                                                                                                        gCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTa                             <  1:39733/122‑1 (MQ=255)                                                                                                                        gCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTa                             >  2:39733/1‑122 (MQ=255)                                                                                                                             aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                                    >  1:877919/1‑110 (MQ=255)                                                                                                                             aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                                    <  2:877919/110‑1 (MQ=255)                                                                                                                             aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATaaaa       >  1:742818/1‑139 (MQ=255)                                                                                                                                  agtaACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGa  >  2:685068/1‑139 (MQ=255)                                                                                                                                  agtaACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGa  >  2:146423/1‑139 (MQ=255)                                                                                                                                    taacaaCATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTAtt                                                                       <  1:1303556/68‑1 (MQ=255)                                                                                                                                    taacaaCATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTAtt                                                                       >  2:1303556/1‑68 (MQ=255)                                                                                                                                       |                                                                                                                                      ACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGA  >  NC_000913/3803934‑3804201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑