Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,842,455	C→T	*445* (TAG→TAA)	adeQ ←	adenine permease, high affinity, adenine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,842,455	0	C	T	100.0%	255.2 / NA	78	*445* (TAG→TAA)	adeQ	adenine permease, high affinity, adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/43);  total (35/43)

TTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCT  >  NC_000913/3842325‑3842581                                                                                                                                   |                                                                                                                               ttttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac                                                                                                                        <  1:817265/139‑1 (MQ=255) ttttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac                                                                                                                        <  2:686762/139‑1 (MQ=255)   ttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                      <  1:652894/139‑1 (MQ=255)       ggCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaat                                                                                                                  <  1:1351420/139‑1 (MQ=255)        gCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaata                                                                                                                 <  2:936219/139‑1 (MQ=255)         ccccGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaataa                                                                                                                >  1:1052005/1‑139 (MQ=255)                        cccGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACa                                                                                                 <  2:1052005/139‑1 (MQ=255)                             aaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                            <  1:310695/139‑1 (MQ=255)                             aaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                            <  1:58361/139‑1 (MQ=255)                                              tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                           <  1:1253281/139‑1 (MQ=255)                                              tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                           <  2:270658/139‑1 (MQ=255)                                              tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                           >  2:1253281/1‑139 (MQ=255)                                               aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcaca                                                                             >  1:1234499/1‑136 (MQ=255)                                               aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcaca                                                                             <  2:1234499/136‑1 (MQ=255)                                                   ccAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGttatta                                                                                                                              >  2:873368/1‑83 (MQ=255)                                                    cAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGtt                                                                     >  2:581454/1‑139 (MQ=255)                                                        gTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGa                                                                 >  1:508130/1‑139 (MQ=255)                                                           gCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcg                                                              >  2:1417787/1‑139 (MQ=255)                                                             gAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcgcg                                                            <  2:642343/139‑1 (MQ=255)                                                                   gatctggATAATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                          >  1:882577/7‑119 (MQ=255)                                                                   gatctggATAATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                          <  2:882577/113‑1 (MQ=255)                                                                     tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                    <  2:508130/139‑1 (MQ=255)                                                                     tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                    <  1:581454/139‑1 (MQ=255)                                                                     tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                    <  2:989784/139‑1 (MQ=255)                                                                          aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGc                                                                                             <  2:1131849/93‑1 (MQ=255)                                                                          aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGc                                                                                             >  1:1131849/1‑93 (MQ=255)                                                                          aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGG‑TCAGATCGCGCCCGCGCCCGGTGc                                              >  1:999639/1‑139 (MQ=255)                                                                               gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                           <  1:1198505/58‑1 (MQ=255)                                                                               gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                           >  2:1198505/1‑58 (MQ=255)                                                                               gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                           <  2:232854/106‑1 (MQ=255)                                                                               gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                           >  1:232854/1‑106 (MQ=255)                                                                               gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                          >  1:1002238/1‑139 (MQ=255)                                                                                    ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaata                                                                                                                 <  2:25965/63‑1 (MQ=255)                                                                                    ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaata                                                                                                                 >  1:25965/1‑63 (MQ=255)                                                                                    ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                       >  2:1330081/1‑89 (MQ=255)                                                                                    ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                       <  1:1330081/89‑1 (MQ=255)                                                                                     tcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcgcg                                                            >  2:1381764/1‑115 (MQ=255)                                                                                     tcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcgcg                                                            <  1:1381764/115‑1 (MQ=255)                                                                                       cTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCATACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatga                                  <  2:1011592/139‑1 (MQ=255)                                                                                       cTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGcc                                                          <  2:269025/115‑1 (MQ=255)                                                                                       cTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGcc                                                          >  1:269025/1‑115 (MQ=255)                                                                                            tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                          <  1:1033488/126‑1 (MQ=255)                                                                                            tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                          >  2:1033488/1‑126 (MQ=255)                                                                                               tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgc                                                       >  1:873128/1‑110 (MQ=255)                                                                                               tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgc                                                       <  2:873128/110‑1 (MQ=255)                                                                                               tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTg                                               <  1:877680/118‑1 (MQ=255)                                                                                               tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTg                                               >  2:877680/1‑118 (MQ=255)                                                                                               tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTAc                          >  1:1385682/1‑139 (MQ=255)                                                                                               tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTAc                          >  2:166479/1‑139 (MQ=255)                                                                                                 aGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                <  2:627692/131‑1 (MQ=255)                                                                                                 aGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                >  1:627692/1‑131 (MQ=255)                                                                                                  gAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGAc                       <  2:1385682/139‑1 (MQ=255)                                                                                                    aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACAt                     <  1:166479/139‑1 (MQ=255)                                                                                                     cTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATa                    <  1:573193/139‑1 (MQ=255)                                                                                                       cATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATaaa                  <  2:194358/139‑1 (MQ=255)                                                                                                        aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCgg                                                 >  2:1396412/1‑107 (MQ=255)                                                                                                        aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCgg                                                 <  1:1396412/107‑1 (MQ=255)                                                                                                         tGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                           <  1:42800/80‑1 (MQ=255)                                                                                                         tGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                           >  2:42800/1‑80 (MQ=255)                                                                                                          gATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc               <  1:1094947/139‑1 (MQ=255)                                                                                                             atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc               >  2:816914/1‑136 (MQ=255)                                                                                                             atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc               <  1:816914/136‑1 (MQ=255)                                                                                                               cTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTg          >  2:970836/1‑139 (MQ=255)                                                                                                                  gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccaccac                                                                                 <  1:680417/65‑1 (MQ=255)                                                                                                                  gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccaccac                                                                                 >  2:680417/1‑65 (MQ=255)                                                                                                                  gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                       >  2:846254/1‑59 (MQ=255)                                                                                                                  gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                       <  1:846254/59‑1 (MQ=255)                                                                                                                  gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTCCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa       >  1:234055/1‑139 (MQ=255)                                                                                                                      cccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc               >  1:526168/1‑127 (MQ=255)                                                                                                                      cccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc               <  2:526168/127‑1 (MQ=255)                                                                                                                       ccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCt  >  1:1148772/1‑139 (MQ=255)                                                                                                                        cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                            >  1:1391250/1‑48 (MQ=255)                                                                                                                        cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                            <  2:1391250/48‑1 (MQ=255)                                                                                                                        cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                     <  1:399331/55‑1 (MQ=255)                                                                                                                        cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                     >  2:399331/1‑55 (MQ=255)                                                                                                                          cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccac                                                                                    >  1:554570/1‑54 (MQ=255)                                                                                                                          cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccac                                                                                    <  2:554570/54‑1 (MQ=255)                                                                                                                          cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTg          >  2:188246/1‑128 (MQ=255)                                                                                                                          cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTg          <  1:188246/128‑1 (MQ=255)                                                                                                                                ttattaATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATa                    >  1:499536/1‑112 (MQ=255)                                                                                                                                   |                                                                                                                               TTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCT  >  NC_000913/3842325‑3842581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑